Mouse Interleukin 4,IL-4 ELISA KIT

Code CSB-E04634m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07750
Lead Time 3-5 working days
Abbreviation IL4
Protein Biological Process 1 Immunity
Target Name interleukin 4
Alias BCGF-1, BCGF1, BSF1, IL-4, MGC79402, B cell growth factor 1|B_cell stimulatory factor 1|lymphocyte stimulatory factor 1
Species Mus musculus (Mouse)
Protein Biological Process 3 B-cell activation
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 90  
Range % 85-96  
1:4 Average % 86  
Range % 80-90  
1:8 Average % 89  
Range % 84-97  
Recovery
The recovery of mouse IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 92 87-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.775 2.757 2.766 2.610  
50 2.546 2.434 2.490 2.334  
25 2.053 2.008 2.031 1.875  
12.5 1.397 1.345 1.371 1.215  
6.25 0.816 0.786 0.801 0.645  
3.12 0.478 0.462 0.470 0.314  
1.56 0.278 0.263 0.271 0.115  
0 0.158 0.154 0.156    
References
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Function Participates in at least several B-cell activation processes as well as of other cell types
Subcellular Location Secreted
Protein Families IL-4/IL-13 family
Database Links

KEGG: mmu:16189

STRING: 10090.ENSMUSP00000000889

UniGene: Mm.276360

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway
Fc epsilon RI signaling pathway
Hematopoietic cell lineage
IL-17 signaling pathway
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation

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