Mouse Interleukin 4,IL-4 ELISA KIT

Code CSB-E04634m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 4
Alternative Names BCGF-1, BCGF1, BSF1, IL-4, MGC79402, B cell growth factor 1|B_cell stimulatory factor 1|lymphocyte stimulatory factor 1
Abbreviation IL4
Uniprot No. P07750
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 90  
Range % 85-96  
1:4 Average % 86  
Range % 80-90  
1:8 Average % 89  
Range % 84-97  
The recovery of mouse IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 92 87-95  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.775 2.757 2.766 2.610  
50 2.546 2.434 2.490 2.334  
25 2.053 2.008 2.031 1.875  
12.5 1.397 1.345 1.371 1.215  
6.25 0.816 0.786 0.801 0.645  
3.12 0.478 0.462 0.470 0.314  
1.56 0.278 0.263 0.271 0.115  
0 0.158 0.154 0.156    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Participates in at least several B-cell activation processes as well as of other cell types
Subcellular Location Secreted
Protein Families IL-4/IL-13 family
Database Links

KEGG: mmu:16189

STRING: 10090.ENSMUSP00000000889

UniGene: Mm.276360

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