Recombinant Mouse Interleukin-4(Il4),partial (Active)

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Code CSB-AP004811MO
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells is less than 2 ng/ml.
Target Names Il4
Uniprot No. P07750
Research Area Immunology
Alternative Names Il4; Il-4Interleukin-4; IL-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1
Species Mus musculus (Mouse)
Source E.coli
Expression Region 23-140aa
Mol. Weight 13.4 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Participates in at least several B-cell activation processes as well as of other cell types
Gene References into Functions
  1. Downregulation of macrophage Irs2 by hyperinsulinemia impairs IL-4-indeuced M2a-subtype macrophage activation in obesity. PMID: 30451856
  2. Ndfip1 preserves Treg lineage stability and immune homeostasis by preventing the expansion of highly proliferative and metabolically active Treg cells and by preventing pathological secretion of IL-4 from Treg cells PMID: 28580955
  3. IL-4/ STAT6 signaling needs to be well adjusted to ensure proper development and function of homing Th2 cells. PMID: 29738764
  4. By establishing that IL-4 is posttranslationally regulated by TRX-promoted reduction of a disulfide bond, our findings highlight a novel regulatory mechanism of the type 2 immune response that is specific to IL-4 over IL-13. PMID: 30104382
  5. The VEGFR1-mediated signaling suppressed IL-4-induced Arg-1 expression. PMID: 29110610
  6. The results obtained in the present study suggest that a signaling pathway mediated by FcRg or the FcRg-Syk axis is commonly required for innate basophil IL-4 responses under conditions mimicking encounters with allergen sources. PMID: 26703455
  7. IL-4Delta2 did not compete with IL-4 for IL-4Ralpha binding and did not interfere with the downstream STAT-6 phosphorylation in T cells. PMID: 28917204
  8. this study shows that IL4 and IL21 cooperate to induce the high Bcl6 protein level required for germinal center formation PMID: 28875978
  9. The complex role of IL-4 in autoimmunity and cholangitis. PMID: 27721424
  10. The results demonstrate that IL-4 can restore insulin sensitivity in adipocytes via mechanisms not associated with induced adipogenesis or de novo formation of lipid depots. PMID: 29738684
  11. Interleukin 4 (IL-4) signaling prevents Chlamydia trachomatis Infection from Inducing upper genital tract (UGT) pathology. PMID: 28765368
  12. In the lung, surfactant protein A (SP-A) enhanced interleukin-4 (IL-4)-dependent macrophage proliferation and activation, accelerating parasite clearance and reducing pulmonary injury after infection with a lung-migrating helminth. In the peritoneal cavity and liver, C1q enhancement of type 2 macrophage activation was required for liver repair after bacterial infection. PMID: 28495878
  13. Data, including data from studies using transgenic mice, suggest that over-expression of IL4 (interleukin 4) in thyroid tissue/cells up-regulates expression of Duox1 (dual oxidase 1), Duoxa1 (dual oxidase maturation factor 1), and Slc26a4 (pendrin) in thyroid tissue/cells; expression of Slc5a5 (sodium-iodide symporter) is down-regulated. PMID: 27599561
  14. we defined a molecular mechanism for IL-4 downregulation of involucrin in keratinocytes, which may play an important role in the pathogenesis of AD. PMID: 26918372
  15. In this study, the effect of continuous IL-4 delivery or bioactive implant coating that constitutively releases a protein inhibitor of CCL2 signaling (7ND) on particle induced osteolysis were studied in the murine continuous femoral intramedullary particle infusion model PMID: 27114284
  16. T follicular helper (Tfh) cells arise in tumor-draining lymph nodes where they produce an abundance of IL4. Deletion of IL4-expressing Tfh cells improves antitumor immunity, delays tumor growth, and reduces the generation of immunosuppressive myeloid cells in the lymph nodes. PMID: 27920023
  17. Findings suggest that interleukin 4 (IL-4) affects anti-tumor immunity and constitutes an attractive therapeutic target to reduce immune suppression in the tumor microenvironment. PMID: 28733709
  18. this study shows that environmental IL-4 plays a role in conditioning early thymic progenitors lineage choice, which would impact T cell development PMID: 28893952
  19. this study shows that eosinophils subvert host resistance to an intracellular pathogen by instigating non-protective IL-4 in CCR2(-/-) mice PMID: 27049063
  20. findings show that during intestinal helminth infection, IL-4 derived from T follicular helper cells is required for IgE class switching and plasmablast formation PMID: 28533444
  21. Data suggest that Il4 (usually released from helper T-cells) induces Cox1 in macrophages at post-transcriptional level; activation of Ampk (catalytic subunit Prkaa1) by metformin blocks Il4-dependent induction of Cox1 and blocks macrophage polarization/activation. (Il4 = interleukin-4; Cox1 = cyclooxygenase 1; Ampk = AMP-activated protein kinase) PMID: 28684424
  22. IL-4 is required for the development of ex-Foxp3 T helper 2 cells. PMID: 28507062
  23. conclude that a state of haploinsufficiency for the Il4 gene locus is specifically relevant for IL-4-dependent IgE responses to allergens with the amount of IL-4 produced in the hemizygous condition falling close to the threshold required for switching to IgE production PMID: 28115531
  24. priming of T helper cells by IL-6-deficient antigen-presenting dendritic cells preferentially leads to accumulation of a subset of Follicular helper T cells characterized by high expression of GATA3 and IL-4. PMID: 27474166
  25. eosinophils drive progression of myocarditis to Inflammatory dilated cardiomyopathy (DCMi), cause severe DCMi when present in large numbers, and mediate this process through IL-4. PMID: 28302646
  26. These data suggest that although IL-4-stimulated alternatively activated macrophages upregulate fatty acid oxidation, fatty acid oxidation is dispensable for macrophage polarization and high-fat diet-induced metabolic dysfunction. Macrophage fatty acid oxidation likely plays a correlative, rather than causative, role in systemic metabolic dysfunction. PMID: 28223293
  27. Excessive IL-4 levels in the mesenteric lymph nodes (MLNs) directly inhibited the induction of aiTregs and caused enteropathy. The aiTregs generated in the attenuation of T cell-dependent food allergic enteropathy may function differently than aiTregs induced in a tolerance model. PMID: 28234975
  28. this study shows that wild-type mice develop an eosinophilic Th2 airway disease in response to Alternaria alternata exposure, whereas IL-4-deficient mice exhibit a primarily neutrophilic response PMID: 27815425
  29. Study showed that the intraperitoneal administration of the exogenous cytokines IFN-gamma (to promote M1 microglia ) and IL-4 (to promote M2 microglia) can correctly modulate the timing of the M1 to M2 ratio to affect epileptogenesis and to improve cognitive function in pilocarpine-induced status epilepticus. PMID: 27956120
  30. These findings indicate that IL-4, a canonical Th2 cell cytokine, can sometimes promote rather than impair Th1 cell-type immune responses PMID: 27298446
  31. Keratinocyte gene expression is critically shaped by IL-4, altering cell fate decisions, which are likely important for the clinical manifestations and pathology of allergic skin disease PMID: 27554818
  32. Data show that lactic acid in tumor microenvironments inhibited interferon-gamma (IFNgamma) and intert=leukin-4 (IL4) productions from NKT cells by inhibiting mammalian target of rapamycin (mTOR) signaling. PMID: 27995420
  33. this study shows that IL-4-mediated control of the precursor population affects the development of virus-specific CD8+ T-cell memory PMID: 27457412
  34. IL-4 secretion by group 2 innate lymphoid cells contributes to the allergic response in food allergy by reducing allergen-specific Treg cell and activating mast cell counts PMID: 27177780
  35. These studies clearly show a crucial role for IL-4 in the induction of airway hyperresponsiveness following Strongyloides venezuelensis infection and for IL-33/ST2 in maintaining airway hyperresponsiveness and lung Th2 responses. PMID: 27102638
  36. we used recombinant herpes simplex virus vector S4IL4 that encode mouse il4 gene to evaluate the therapeutic potential of IL-4 in naloxone-precipitation morphine withdrawal (MW). One week after microinjection of the vector S4IL4 into the PAG LacZ or mouse IL-4 immunoreactivity in the vlPAG was visualized. ELISA assay showed that vector S4IL4 into the PAG induced the expression of IL-4 PMID: 28206989
  37. this study shows that IL-4 is increased in the brain of T cell receptor transgenic mice, which exhibit impaired memory and adult hippocampal neurogenesis PMID: 27432189
  38. this study shows that il-4 plays an important role in ESAT-6-induced MCP-1 production by macrophages, and suggest a pathway with significance in tuberculosis pathogenesis PMID: 27154637
  39. indicate that Siglec-9 affects several different signaling pathways in IL-4-stimulated macrophages, which resulted in enhanced induction of Arg1 in Siglec-9-expressing RAW264 cells PMID: 26540411
  40. Oct-1 and Oct-2 bound within the Il4 promoter region and the Th2 LCR PMID: 26840450
  41. Loss of IL-4 promoted expression of M1 microglia/macrophage markers and impaired expression of M2 markers after transient or permanent middle cerebral artery occlusion. PMID: 26732561
  42. These results indicate a positive role of Batf in promoting the generation of pro-allergic IL-4-producing T-follicular helper cells. PMID: 26278622
  43. IL-4 induces miR-142-5p and downregulates miR-130a-3p in macrophages, regulating macrophage profibrogenic gene expression in chronic inflammation. PMID: 26436920
  44. these findings underscore the important collaboration between IL-4 and IL-21 in shaping T-dependent B cells antibody responses. PMID: 26491200
  45. IL-4 KO mice display state, but not trait, anxiety suggesting that reductions in endogenous anti-inflammatory bioactives can engender subtypes of anxiety PMID: 25772794
  46. physiologic doses of interleukin-4 (IL-4) and interleukin-13 (IL-13) have profound anti-lymphangiogenic effects and potently impair LEC survival, proliferation, migration PMID: 26039103
  47. Concerted activity of IgG1 antibodies and IL-4/IL-25-dependent effector cells trap helminth larvae in the tissues following vaccination with defined secreted antigens, providing sterile immunity to challenge infection. PMID: 25816012
  48. may be an important factor in providing 1,25D3-induced protection from experimental autoimmune encephalomyelitis PMID: 25574039
  49. IL-4-producing DCs are induced under some Th2-provoking situations, and they should play important roles in initiation of Th2 response. PMID: 26363056
  50. RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4. PMID: 26416964
  51. This study demonstrated that young IL-4 knockout mice, the excitability of nociceptive primary afferents and/or spinal nociceptive neurons is greater than in wide type mice. PMID: 26079835
  52. results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators PMID: 25264308
  53. deficiency induced reduction in cytokine response in macrophages, and an increased hepatic IFN-gamma response, improvement in mouse survival, attenuation of granulomas PMID: 25291432
  54. Data indicate that signal transducer and activator of transcription 3 (Stat3) can repress the expression of transcription factor Bcl6 and interleukin-4 (IL-4) in follicular helper T (TFH) cells. PMID: 26188063
  55. This study not only establishes a causal relationship between IL-4 and cardiac fibrosis/dysfunction, but also reveals a critical role for IL-4 in angiotensin II-induced cardiac damage. PMID: 26195478
  56. In response to ischemia, necrotic brain cells discharge factors that polarize MPhi to a M1-like phenotype. We show that, to offset this M1-like polarization process, sublethally ischemic neurons may instead secrete a potent M2 polarizing cytokine, IL4. PMID: 26269636
  57. data provide new insights into the molecular mechanisms governing IL-4Ralpha-induced responses, and may provide new therapeutic tools to target IL-4 in allergy and asthma PMID: 26124135
  58. IL-4 is required for female neuroprotection from focal ischemia compared to male mice. PMID: 26130091
  59. Myocardial natural killer cell-derived IFNG/IL-4 is critical for the differential polarization of macrophages in coxsackie B virus-induced myocarditis. PMID: 25123338
  60. Data indicate that NLR family pyrin domain containing 3 (NLRP3) bound the interleukin-4 (Il4) promoter and transactivated it in conjunction with the transcription factor IRF4. PMID: 26098997
  61. intestinal Th2 priming is initiated by an autocrine/paracrine acting CD4(+) Th cell-intrinsic IL-4 program that is controlled by DC OX40L, and not by NKT, gammadelta T, or ILC cells. PMID: 24781052
  62. Zinc-binding MT1 and MT2 are key modulators of Fc epsilonRI-induced basophil production of IL-4. PMID: 25801306
  63. neutrophil-derived Ym1 protein uptake distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing PMID: 25307347
  64. IL-4-deficient mice that underwent cecal ligation and puncture were resistant to secondary pulmonary Pseudomonas aeruginosa infection. PMID: 25489003
  65. NLRP12 is an intrinsic negative regulator of T-cell-mediated immunity via altered NF-kappaB regulation and IL-4 production as key mediators of NLRP12-associated disease. PMID: 25888258
  66. Interleukin-4 regulates eomesodermin in CD8+ T cell development and differentiation PMID: 25207963
  67. Activation of invariant natural killer T cells impedes liver regeneration by way of both IFN-gamma- and IL-4-dependent mechanisms. PMID: 24623351
  68. gammadelta T cells, controlled by their own cross-talk, affect IL-4 production, B-cell activation, and B-cell tolerance. PMID: 25535377
  69. results indicate that IL-4 mediates neuroprotection and recovery of the injured CNS PMID: 25607842
  70. p38 MAPK has a critical role in IL-4-induced alternative activation of peritoneal macrophages. PMID: 25328047
  71. This study is the first to demonstrate that increased susceptibility to peanut allergy in offspring mice of mothers with peanut allergy is associated with epigenetic alteration of the Il4 gene promoter. PMID: 25441650
  72. we delineate the detailed molecular pathway for IL-4 up-regulation of IL-19 in keratinocytes, which may play an important role in AD pathogenesis. PMID: 24943510
  73. elevated in liver during halothane-induced liver injury PMID: 24723460
  74. Because NFATc2 translocation into the nucleus occurs in an all-or-none fashion, dependent on complete dephosphorylation by calcineurin, NFATc2 controls the frequencies of cells reexpressing Il4 PMID: 25037220
  75. These results indicate that RNF13 deficiency promotes skeletal muscle regeneration via the effects on satellite cell niche mediated by IL-4 and IL-6. PMID: 24563216
  76. Although basophils were recruited into liver granulomas, they appeared to be dispensable as a source of IL-4/IL-13 both for differentiation of Th2 cells and for prevention of weight loss and mortality. PMID: 25172500
  77. IL-4 modulates and counteracts pro-inflammatory stimulation induced by TLR7 and TLR9. PMID: 24489947
  78. In allergic airway inflammation, IL-4 signaling determines macrophage activation state. PMID: 24907978
  79. These findings suggest that some naive CD8(+) T cells may be preprogrammed by weak homeostatic TCR signals in the presence of IL-4 to become memory phenotype cells with the ability to elaborate effector function rapidly. PMID: 24620029
  80. Interleukin-4 deficiency protects mice from acetaminophen-induced liver injury and inflammation by prevention of glutathione depletion. PMID: 24100592
  81. KLF13 directly binds to IL-4 promoter regions and synergizes with c-Maf to positively regulate IL-4 expression. PMID: 24821970
  82. Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population. PMID: 24795452
  83. IL-4 regulates Bim expression and promotes B cell maturation in synergy with BAFF conferring resistance to cell death at negative selection checkpoints. PMID: 24835393
  84. Stat6 positively regulates a large number of lysosomal genes in an IL-4-dependent manner. PMID: 24314139
  85. A unique DNA methylation signature defines a population of IFN-gamma/IL-4 double-positive T cells during helminth infection. PMID: 24578067
  86. IL-4 from basophils has an important role in the natural helper-derived cytokine and chemokine expression, subsequently leading to protease allergen-induced airway inflammation. PMID: 24837103
  87. suggest that basophils are rapidly recruited to the airways of naive mice following initial fungal allergen exposure, produce IL-4 and influence the development of the adaptive immune response PMID: 24750795
  88. Data suggest that inducible costimulator (ICOS) costimulation-dependent translational control may ensure targeted delivery of IL-4 to cognate B cells during T-B collaborations in the germinal center. PMID: 24486724
  89. These findings point to a previously unappreciated role for helminth-induced IL-4 in impairment of induced natural killer T cell-mediated clearance of bacterial coexposure. PMID: 24643536
  90. Data indicate that both IL-4 and IL-4 receptor (IL-4Ralpha) signaling pathways are dispensable for the development, maintenance and functional differentiation of natural killer T cells. PMID: 23990998
  91. Data indicate that IL-4 neutralization led to an early increase in Th1 cell recruitment to the inflamed dermis. PMID: 23991011
  92. inhibits inflammatory cytokine gene expression by dendritic cells through the histone deacetylation PMID: 24139608
  93. data show that the absence of IL-4 is sufficient to induce mild preeclampsia-like symptoms in mice due to excessive inflammation PMID: 23552130
  94. Activated iNKT cells rapidly release IL-4, which promotes neutrophil survival and hepatitis but also sequentially produce IFN-gamma, which acts in a negative feedback loop to ameliorate iNKT hepatitis by inducing neutrophil apoptosis. PMID: 23686838
  95. This study identifies a regulatory function for IRE1alpha in the promotion of IL-4 in T cells. PMID: 24100031
  96. The IL-4 pathway of proliferation may have developed as an alternative to CSF-1 to increase resident macrophage numbers without coincident monocyte recruitment. PMID: 24101381
  97. Steady-state production of IL-4 modulates immunity in mouse strains and is determined by lineage diversity of the invariant NKT cells. PMID: 24097110
  98. study identified a novel activation pathway in murine eosinophils that is induced by IL-33 and differentially dependent upon an IL-4 auto-amplification loop. PMID: 24043894
  99. IL-33 signalling via ST2, by inducing an IL-4-dependent immune response, may be a major pathogenic factor in the exacerbation of ulcerative colitis. PMID: 23582173
  100. Our objective was to elucidate mechanisms involved in modulating arginase I induction by IL-4 in murine M2 macrophages PMID: 23913966

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Subcellular Location Secreted
Protein Families IL-4/IL-13 family
Database Links

KEGG: mmu:16189

STRING: 10090.ENSMUSP00000000889

UniGene: Mm.276360

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