Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit

Instructions
Code CSB-EL012775MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lipopolysaccharide binding protein
Alternative Names LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
Abbreviation LBP
Uniprot No. Q61805
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-92  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 98  
Range % 94-101  
1:8 Average % 93  
Range % 90-96  
Recovery
The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 96 91-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
800 2.922 2.974 2.948 2.803  
400 2.166 2.159 2.163 2.018  
200 1.447 1.448 1.448 1.303  
100 0.913 0.925 0.919 0.774  
50 0.524 0.565 0.545 0.400  
25 0.335 0.312 0.324 0.179  
12.5 0.253 0.258 0.256 0.111  
0 0.140 0.150 0.145    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria
Gene References into Functions
  1. The LBP gene is a macrophage-specific LXR target that promotes foam cell survival and atherogenesis. PMID: 24671012
  2. LBP is a critical factor in the development of non-alcoholic fatty liver disease. PMID: 27404046
  3. LBP is a proinflammatory soluble adipokine that acts as a brake for adipogenesis, strengthening the negative effects of palmitate and LPS on adipocyte differentiation. PMID: 26201685
  4. Differential expression of LBP and TGFB1, along with other genes, in different mesenchymal stromal cells preparations, produces the variable responses to external stimuli. PMID: 24664302
  5. findings describe a novel role for LBP in normal hippocampal development and raise the possibility that some of the behavioral sequelae of early life stress are mediated by reduced expression of LBP during a critical period of neurodevelopment. PMID: 21993208
  6. The amino acid sequence for two mimic epitopes of the inflammatory site of LBP were determined to be WKAQKRFMKKSG and LKTRKLFWKYKD. PMID: 20833181
  7. LBP plays an important role in early alcohol-induced liver injury by enhancing LPS-induced signal transduction, most likely in Kupffer cells. PMID: 11884468
  8. LBP plays an important role in resistance to lethal Salmonella peritonitis (but not to oral or intravenous infection) by facilitating the role of neutrophils in mediating acute inflammation. PMID: 12055258
  9. role LBP plays in local pulmonary immune defenses to bacterial challenge PMID: 12353926
  10. In the case of intraperitoneal infection of LBP-deficient mice with Salmonella typhimurium, no local inflammatory response is evident, neutrophil influx is delayed, and the mice succumb to the infection. PMID: 12370383
  11. plays an essential role in the innate immune response to Gram-positive pneumococci PMID: 12932360
  12. Locally produced LBP is an essential component of an effective innate immune response to E. coli peritonitis. PMID: 14638760
  13. LBP is one of the critical molecules regulating the acute and chronic airway response to inhaled lipopolysaccharides (LPS). PMID: 15356561
  14. LBP knockout mice are protected from toxicity with a decrease in hepatic necrosis following acetaminophen challenge. This suggests a novel role for LBP in modulating acetaminophen-induced liver injury. PMID: 15619225
  15. LBP does not contribute to an effective host response in Mycobacterium tuberculosis infection in mice. PMID: 15993364
  16. LBP per se possesses a protective effect on acute necrotizing pancreatitis PMID: 16163057
  17. In conclusion, hemorrhagic shock and resuscitation to mice cause severe, LBP-mediated hepatocellular damage. PMID: 16614372
  18. Oxidized phospholipid inhibition of toll-like receptor (TLR) signaling is restricted to TLR2 and TLR4: roles for CD14, LPS-binding protein, and MD2 as targets for specificity of inhibition. PMID: 18559343
  19. LPS-binding protein mediates LPS-induced liver injury and mortality in the setting of biliary obstruction. PMID: 18948440
  20. Marked concomitant increases in plasma LBP and endotoxin-like activity following murine trypanosome infection might play an important role in the pathogenesis of trypanosomosis PMID: 19022463
  21. Paneth cells express LBP together with other proteins acting in the innate immune response of the gut, such as lysozyme, defensins and intelectin. PMID: 19234712
  22. Innate defense against lethal peritoneal infection with Salmonella requires the direct proinflammatory involvement of LPS-binding protein (LBP), a major nonredundant function of LBP in this model. PMID: 11466385

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Subcellular Location Secreted, Cytoplasmic granule membrane
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Database Links

KEGG: mmu:16803

STRING: 10090.ENSMUSP00000016168

UniGene: Mm.218846

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