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| Code | CSB-EL012775MO |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 96 | 91-102 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This mouse LBP ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse LBP in serum, plasma, and tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of LBP bound in the initial step.
LBP is an acute-phase protein mainly produced in hepatocytes that binds to bacterial lipopolysaccharide (LPS) thus initiating an innate host response. LBP combines with LPS and then recruits CD14 and MD-4/2 complex, eventually leading to the activation of signal transduction pathways and the generation of cytokines and other pro-inflammatory mediators. Circulating LBP exerts immunologic function in a concentration-dependent manner. Subnormal concentrations of LBP augment phagocytosis and scavenging of LPS from blood. However, high levels of LBP decrease the release of pro-inflammatory cytokines. LBP is a potential acute-phase biomarker for monitoring the progress of severe sepsis, infectious endocarditis, and cardiovascular disease.
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