Mouse Lymphocyte activation gene 3 protein(LAG3) ELISA kit

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Target Background

Name: Mouse Lymphocyte activation gene 3 protein(LAG3) ELISA kit
Brand: CUSABIO
SKU: CSB-EL012719MO

Product Details

Target Name

lymphocyte-activation gene 3

Alternative Names

Lag3 ELISA Kit; Lymphocyte activation gene 3 protein ELISA Kit; LAG-3 ELISA Kit; Activation-induced cytidine deaminase-linked autoimmunity protein ELISA Kit; Aida ELISA Kit; CD antigen CD223) [Cleaved into: Secreted lymphocyte activation gene 3 protein ELISA Kit; sLAG-3)] ELISA Kit

Abbreviation

LAG3

Uniprot No.

Q61790

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 ng/mL-80 ng/mL

Sensitivity

0.31 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LAG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	89-97
1:10	Average %	104
	Range %	98-107
1:20	Average %	91
	Range %	84-94
1:40	Average %	99
	Range %	94-102

Recovery

The recovery of mouse LAG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	95	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.859	2.745	2.802	2.694
40	2.042	2.078	2.060	1.952
20	1.133	1.112	1.123	1.015
10	0.663	0.643	0.653	0.545
5	0.372	0.382	0.377	0.269
2.5	0.275	0.281	0.278	0.170
1.25	0.184	0.178	0.181	0.073
0	0.109	0.107	0.108

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This mouse LAG3 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse LAG3 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the LGA3 levels in the sample and is measured at 450 nm through a microplate reader.

LAG3 is mainly expressed in activated T and natural killer (NK) cells and was identified as a marker for the activation of CD4⁺ and CD8⁺ T cells. Under pathological conditions, such as chronic inflammation or tumor environment, increased LAG3 expression on T cells was observed in combination with other inhibitory receptors such as PD-1, T cell immunoglobulin, and TIGIT, TIM3, CD160, 2B4, which finally led to T cell dysfunction. LAG3 is an important immune checkpoint with relevance in cancer, infectious disease, and autoimmunity. Furthermore, LAG3 was mainly found on tumor-infiltrating Tregs in many types of cancer when compared with non-malignant peripheral cells.

Customer Reviews and Q&A

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■ Q&A

Is the mouse LAG3 elisa kit (#CSB-EL012719MO) specific for the soluble portion of the LAG-3 receptor or is it for an epitope in a different region?

CSB-EL012719MO Mouse Lymphocyte activation gene 3 protein (LAG3) ELISA kit is designed against extracellular domain. Pls refer to: https://www.uniprot.org/uniprotkb/Q61790/ Sorry we are not clear about the specific epitope.

Target Background

Function
(From Uniprot)

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear.; May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

Gene References into Functions

upregulated in the central nervous system by intraepithelial lymphocytes from the gut epithelium of myelin oligodendrocyte glycoprotein(35-55)-specific T-cell receptor transgenic mice PMID: 27198196
thi study shows that LAG-3 directly modulates the size of the T cell response and support the use of LAG-3 blockade regimens to enhance CD8+ T cell responses PMID: 27206765
Study suggests that expression of the inhibitory receptors PD-1 and LAG-3 on CD4(+) T cells and their reduced IL-2 production are common characteristic features of Plasmodium infection. PMID: 26696540
An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell-suppressive function and therapeutic efficacy. PMID: 26013006
LAG3 and PD1 co-inhibitory molecules have roles in collaborating to limit CD8+ T cell signaling and dampen antitumor immunity in a murine ovarian cancer model PMID: 26318293
Poxvirus-Based Active Immunotherapy with PD-1 and LAG-3 Dual Immune Checkpoint Inhibition Overcomes Compensatory Immune Regulation, Yielding Complete Tumor Regression in Mice. PMID: 26910562
Lymphatic endothelial cells (LECs) serve as an antigen reservoir for CD4 T-cell tolerance, and MHC-II molecules on LECs are used to induce CD8 T-cell tolerance via LAG-3. PMID: 25857745
LAG-3 expression on Tconv cells makes them more susceptible to Treg based suppression, and also regulates the development of a TH1 T-cell response. PMID: 25372844
LAG-3 exerts an important regulatory effect on autoimmunity. PMID: 25122007
Lag-3 is an important regulatory molecule involved in alloreactive T cell proliferation and activation after bone marrow transplantation. PMID: 24475140
these results define a strong synergy between the PD-1 and LAG-3 inhibitory pathways in tolerance to both self and tumor antigens. PMID: 22186141
We conclude that LAG-3 is necessary for regulating CD4(+) and CD8(+) T cell function during autoimmune diabetes PMID: 21873518
LAG-3 is required for long-term peripheral CD8 but not CD4 T-cell tolerance and this requirement is CD8 cell-extrinsic. PMID: 21422469
LAG-3 acts synergistically with PD-1 and/or other immunoregulatory genes to prevent autoimmunity in mice. PMID: 21300912
This significant intracellular storage of LAG-3 appears to facilitate its rapid translocation to the cell surface following T-cell activation, which was much faster for LAG-3 than CD4 PMID: 20391435
blockade of LAG-3 did not rescue cytokine production by virus-specific CD8 T cells and did not alter the virus titer in various organs PMID: 19880580
Lag3 inhibits CD4-dependent, but not CD4-independent, T cell function via its cytoplasmic domain. PMID: 12421911
LAG-3 regulates the expansion of activated T cells. PMID: 12672063
LAG-3 activates antigen-presenting cells through MHC class II signalling, leading to increased antigen-specific T-cell responses in vivo. PMID: 14644131
data suggest that LAG-3 negatively regulates T cell expansion and controls the size of the memory T cell pool PMID: 15100286
LAG-3 marks regulatory T cell populations and contributes to their suppressor activity PMID: 15485628
LAG-3 is cleaved within the D4 transmembrane domain connecting peptide into two fragments that remain membrane associated. Modulation of LAG-3 cleavage may contribute to the function of this key regulatory T cell protein. PMID: 15557174
LAG-3 negatively regulates T cell homeostasis by regulatory T cell-dependent and independent mechanisms. PMID: 15634887
LAG-3 is a new marker of T cell induced B cell activation. PMID: 15971272
Metalloprotease-mediated cleavage of LAG-3 controls T-cell proliferation and immune response. PMID: 17245433
Data demonstrate a direct role for LAG-3 on CD8+ T cells and suggest that LAG-3 blockade may be a potential cancer treatment. PMID: 17932562
a novel ITAM-mediated inhibitory signaling pathway in DCs triggered by MHC II engagement of LAG-3, providing a molecular mechanism in which regulatory T cells may suppress via modulating DC function. PMID: 18424711
LAG-3 plays an important but selective cell intrinsic and cell extrinsic role in plasmacytoid dendritic cells biology PMID: 19201841
signaling through the PD-1 and LAG-3 pathways have distinct functional consequences to CD8 T cells under tolerizing conditions PMID: 19454660

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Subcellular Location

[Lymphocyte activation gene 3 protein]: Cell membrane; Single-pass type I membrane protein.; [Secreted lymphocyte activation gene 3 protein]: Secreted.

Tissue Specificity

Primarily expressed in activated CD4(+) and CD8(+) T-cells. Also expressed in a subset of regulatory T-cells (Tregs), such as natural CD4(+)CD25(+) Tregs. Also expressed on plasmacytoid dendritic cells (pDCs).

Database Links

KEGG: mmu:16768

STRING: 10090.ENSMUSP00000032217

UniGene: Mm.4528

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Code	CSB-EL012719MO
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.