LAG3

LAG-3 is an immunoinhibitory transmembrane protein comprised of key components, including four immunoglobulin superfamily domains in the extracellular region, a transmembrane domain, and serine phosphorylation sites in the intracellular domain. Functionally, LAG-3 primarily interacts with ligands such as MHC II and galectin-3 to suppress T cell activation and cytokine secretion, thereby maintaining immune homeostasis. In terms of signaling, LAG-3 engagement with its ligands triggers intracellular interactions with downstream signaling molecules, modulating the activation state of T cells. In cancer immunotherapy, LAG-3 serves as a crucial target, and blocking or inhibiting LAG-3 can potentiate T cell-mediated tumor killing. Therefore, elucidating the structure, function, and signaling pathways of LAG-3 holds significant promise for the development of novel immunotherapy strategies.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human LAG3 at 2 μg/ml can bind Anti-LAG3 recombinant antibody(CSB-RA012719MA1HU). The EC₅₀ is 2.306-2.731 ng/mL.

LAG3 Recombinant Monoclonal Antibody (CSB-RA012719MA1HU)

Validated Data

IHC image of CSB-RA012719MA1HU diluted at 1：100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

The Binding Activity of Human LAG3 with Anti-LAG3 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human LAG3(CSB-MP012719HU3) at 2 μg/mL can bind Anti-LAG3 recombinant antibody. The EC₅₀ is 2.306-2.731 ng/mL.

Human Lymphocyte activation gene 3 protein(LAG3) ELISA kit (CSB-EL012719HU)

Validated Data

Code: CSB-EL012719HU

Size: 96T,5×96T,10×96T

Sensitivity: 0.039 ng/mL

Detection Range: 0.156 ng/mL-10 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following LAG3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

LAG3 Antibodies

LAG3 Proteins

LAG3 ELISA Kits

LAG3 Antibodies

LAG3 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA012719GA01HU	LAG3 Antibody	Human,Mouse	ELISA,WB
CSB-PA012719LA01HU	LAG3 Antibody	Human	ELISA, WB, IF
CSB-PA012719LC01HU	LAG3 Antibody, FITC conjugated	Human
CSB-PA012719LD01HU	LAG3 Antibody, Biotin conjugated	Human	ELISA
CSB-PA005868	LAG3 Antibody	Human	WB, ELISA
CSB-PA172061	LAG3 Antibody	Human	ELISA,WB
CSB-MA012719A0m	LAG3 Monoclonal Antibody	Human	ELISA, WB, IHC, FC
CSB-RA012719MA1HU	LAG3 Recombinant Monoclonal Antibody	Human	ELISA, IHC

LAG3 Proteins

LAG3 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP012719HU1 CSB-BP012719HU1 CSB-MP012719HU1 CSB-EP012719HU1-B	Recombinant Human Lymphocyte activation gene 3 protein (LAG3), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-CF012719HU	Recombinant Human Lymphocyte activation gene 3 protein (LAG3)	in vitro E.coli expression system
CSB-EP012719HU1	Recombinant Human Lymphocyte activation gene 3 protein (LAG3), partial	E.coli

LAG3 ELISA Kit

LAG3 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL012719HU	Human Lymphocyte activation gene 3 protein(LAG3) ELISA kit	serum, plasma, tissue homogenates	0.039 ng/mL

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LAG3

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