Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Code CSB-E07947m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse SERPINE1 ELISA Kit was designed for the quantitative measurement of Mouse SERPINE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.

Target Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Alternative Names
Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
Abbreviation SERPINE1
Uniprot No. P22777
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.9 ng/mL-60 ng/mL
Sensitivity 0.22 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-95
1:2Average %95
Range %90-100
1:4Average %94
Range %88-102
1:8Average %96
Range %91-103
Recovery
The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-101
EDTA plasma (n=4)9592-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
603.033 3.108 3.071 2.894
302.476 2.557 2.517 2.340
151.887 1.785 1.836 1.659
7.51.117 1.124 1.121 0.944
3.750.711 0.719 0.715 0.538
1.80.488 0.468 0.478 0.301
0.90.329 0.332 0.331 0.154
00.178 0.176 0.177  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. Involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
Subcellular Location Secreted.
Protein Families Serpin family
Database Links

STRING: 10090.ENSMUSP00000039586

UniGene: Mm.250422

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