Pig Renin ELISA Kit

Protocol MSDS

Code	CSB-E17914p
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

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Product Details

Target Name

Renin

Alternative Names

FLJ10761, HNFJ2, angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal

Abbreviation

REN

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	92
	Range %	85-102
1:4	Average %	93
	Range %	83-99
1:8	Average %	95
	Range %	90-110

Recovery

The recovery of pig renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-102
EDTA plasma (n=4)	94	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	1.973	1.997	1.985	1.867
2000	1.635	1.643	1.639	1.521
1000	1.208	1.197	1.203	1.085
500	0.805	0.796	0.801	0.683
250	0.549	0.544	0.547	0.429
125	0.361	0.356	0.359	0.241
62.5	0.255	0.259	0.257	0.139
0	0.116	0.119	0.118

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Pig REN ELISA Kit was designed for the quantitative measurement of Pig REN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 1.56 pg/mL.

Customer Reviews and Q&A

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■ Q&A

I have a customer who interested in both Human renin, REN ELISA Kit CSB-E08701h & Pig Renin ELISA Kit CSB-E17914p
They would like to know if they can use whole blood in these two kits?

Thanks for your inquiry!
Renin, angiotensin constitute renin-angiotensin system (RAS) or renin, angiotensin, aldosterone constitute renin-angiotensin-aldosterone system (RAAS) together to participate in body fluid balance.
Both kits are suitable to serum and plasma samples.They are for quantitative measurement of renin. To test whole blood is not advised.
Pls let me know if you have any further questions. Thank you.

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Pig Renin ELISA Kit

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