Rat Renin ELISA Kit

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Target Background

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Product Details

Description

The Rat Renin ELISA Kit is the perfect solution for researchers looking for a reliable and accurate assay to measure Renin levels in serum, plasma, and tissue homogenates of Rattus norvegicus (Rat). Renin, encoded by the Ren1 gene, is a key regulator of the renin-angiotensin-aldosterone system and plays a critical role in blood pressure regulation.

With a detection range of 15.6 mU/mL to 1000 mU/mL and a sensitivity of 3.9 mU/mL, this ELISA kit provides highly precise and reproducible measurements of Renin levels. The assay principle is quantitative, and the measurement method is a sandwich assay, making it easy to use and highly reliable. The assay time ranges from 1-5 hours, and sample volume requirements are minimal, with 50-100ul of sample volume required.

This kit has been extensively cited, with over 10 published papers referencing its use. The Rat Renin ELISA Kit is ideal for researchers studying cardiovascular physiology and related fields, providing reliable and accurate measurements of Renin levels in biological samples.

Target Name

Renin

Alternative Names

Ren1 ELISA Kit; Ren ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit

Abbreviation

REN

Uniprot No.

P08424

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 mU/mL-1000 mU/mL

Sensitivity

3.9 mU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	90
	Range %	85-94
1:4	Average %	99
	Range %	92-104
1:8	Average %	88
	Range %	84-92

Recovery

The recovery of rat renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
1000	2.497	2.382	2.440	2.254
500	1.773	1.641	1.707	1.521
250	1.152	1.199	1.176	0.990
125	0.693	0.738	0.716	0.530
62.5	0.479	0.515	0.497	0.311
31.2	0.318	0.309	0.314	0.128
15.6	0.234	0.217	0.226	0.040
0	0.191	0.180	0.186

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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■ Q&A

3.9 mU/mL, what does mU stand for?

The representation form of this kit is the active unit, the detection is the content, and the definition of the active unit belongs to the internal traceability reference, which is not convenient to provide to the outside. The representation form of this active unit is also recognized by many authoritative journals and magazines and can be used directly.

Target Background

Function (From Uniprot)	Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Gene References into Functions	The influence of intracellular renin on the inward calcium current in isolated smooth muscle cells from SHR mesenteric arteries was investigated... intracellular renin in vascular resistance vessels from SHR due to internalization or expression, contributes to the regulation of vascular tone and control of peripheral resistance-an effect independently of Ang II. PMID: 27545826 An alternative promoter in intron1 of the renin gene is regulated by glucose starvation via SRF. PMID: 28715805 renin deficiency leads to significant dysregulation of ion transporters. PMID: 27443990 Renal ischemia/reperfusion injury induces renin response in the kidney. Renin production is more explicit in males than in females which, via increased angiotensin II production, might explain differences in renal vessel regulation between the two genders. PMID: 27090360 Isolated perfused hearts obtained from transgenic rats overexpressing cytosolic renin exhibited a 50 % reduction of infarct size after ischemia-reperfusion injury. PMID: 26256830 plasma level elevated in experimental colitis PMID: 26660905 elevated levels of circulating (pro)renin in model of pre-eclampsia PMID: 25767135 prorenin has distinct pro-angiogenic and pro-inflammatory effects on retinal cells PMID: 25707593 Report alterations in adrenal gland aldosterone/corticosterone biosynthesis in the renin knockout rat. PMID: 25394830 at least 4 BP loci reside within the 30-cM chromosome 13 BP quantitative trait locus that includes Renin PMID: 23460292 Up-regulation of renin might contribute to abnormal programming of renal growth in rats exposed to postnatal early overnutrition. PMID: 21752621 An association of post-natal overfeeding and high-fat diet increases plasma renin activity and adipose (pro)renin receptor expression. PMID: 21483231 This study examined angiogenic function and genomic structure of regions surrounding the renin gene. PMID: 21521778 Although plasma renin activity was suppressed in the angiotensinogen II-infused rats, renin content in the renal medulla was increased. Renin levels in the urine increased. PMID: 21865264 Renin released from mast cells activated by circulating MCP-1 initiates the microvascular phase of the systemic inflammation of alveolar hypoxia. PMID: 21963836 The transfer of chromosome 13 from BN to SS rats (to produce the SS.13BN strain) has cardiovascular effects. PMID: 22020141 Imidazolone deposition and renin production in peritubular capillary endothelial cells may play a role in the development of tubulointerstitial fibrosis in insulin-resistant hypertensive rats. PMID: 21484732 Cu/Zn SOD expression was significantly higher in cerebral arteries from Ren1-Brown Norway congenic rats vs. those from Dahl salt-sensitive rats PMID: 21331057 The development of the first rat knockout in the renin-angiotensin system emphasizes the role of renin in BP regulation and kidney function even in the low-renin SS rat. PMID: 21242461 Dahl SS renin allele plays a crucial role in endothelial dysfunction present in the cerebral circulation of the Dahl SS rat, even in the absence of elevated dietary salt intake. PMID: 20852041 The data demonstrate that a cytosolic renin exists in cardiomyocytes, which in contradiction to secretory renin protects from necrosis but increases apoptosis. PMID: 18671756 Nitric oxide synthase mRNA levels do not correlate with gene expression of renin in selected brain areas PMID: 11702851 Coronary dilatation reserve in experimental hypertension and chronic heart failure: effects of blockade of the renin-angiotensin system PMID: 11903302 cellular location of thymic renin is mainly in macrophages and epithelial cells and is greater in hypertensive rats PMID: 12242043 mRNA expression is related to renal dysfunction in tacrolimus-induced acute nephrotoxicity. PMID: 12469222 Intracranial renin alters gustatory neural responses in the nucleus of the solitary tract of rats. PMID: 12511427 cyclooxygenase 2 activity controls the renin-angiotensin system and contributes to elevated renin in the postnatal period PMID: 12560203 renin-specific antibodies demonstrate that cardiac mast cells contain renin PMID: 15342908 Results suggest that the nonproteolytic activation of prorenin may be a significant mechanism of diabetic nephropathy. PMID: 15489960 show that the intracellular domain of Notch1, transcription factor Ets-1, and homeo box D10 (HOXD10).pre B-cell leukemia transcription factor 1(PBX1b).PREP1 activate the rat renin promoter cooperatively PMID: 15792957 the augmentation of distal nephron renin in ANG II-infused hypertensive rats is AT1 receptor mediated PMID: 15870381 Angiotensin II-mediated oxidative stress promotes myocardial tissue remodeling in the transgenic (mRen2) 27 Ren2 rat. PMID: 17440033 Effect of the renin gene on skeletal muscle angiogenesis induced by electrical stimulation. PMID: 18198281 Release of mast cell renin triggers locally produced angiotensin II-induced contraction of rat bronchial rings in vitro. PMID: 18202178 in a rat model of diabetes, prorenin content and release from the collecting duct may be more important than the juxtaglomerular apparatus in contrast to the existing paradigm PMID: 18413493 Upregulation of distal tubular renin helps to explain how sustained intrarenal angiotensin II formation occurs even during juxtaglomerular renin suppression PMID: 18426992 augmented activation of the renin-angiotensin system may be related to enhanced sympathetic outflow observed in sedentary animals and may contribute to increased risk of cardiovascular disease in the sedentary population PMID: 18509102 Renin inhibition attenuates insulin resistance, oxidative stress, and pancreatic remodeling in the transgenic Ren2 rat. PMID: 18653711 Exon(1A-9) renin transcript (1) is translated into a functionally active intracellular protein; (2) is targeted to the cytoplasm rather than being sorted to the secretory pathways and (3) is functionally active, regulating aldosterone production. PMID: 18782187 Adipose tissue may control its own local renin concentration independently of plasma renin as a potential mechanism for maintaining a functional local adipose renin-angiotensin system. PMID: 19050177 mineralocorticoid receptor antagonism attenuates glomerular/podocyte remodeling and albuminuria, in part through reductions in redox-mediated impairment of insulin metabolic signaling in Ren2 rats PMID: 19261739 Ang peptide formation consequent to the increased prorenin levels prevented reduction of Ang II levels after binephrectomy and was likely to have contributed to hypertension, cardiac hypertrophy, and suppression of kidney renin levels in transgenic rats PMID: 19841286 Show More Hide All
Subcellular Location	Secreted. Membrane.
Protein Families	Peptidase A1 family
Database Links	KEGG: rno:24715 STRING: 10116.ENSRNOP00000003951 UniGene: Rn.9831

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Check Code：

Code	CSB-E08702r
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.