Rat Renin ELISA Kit

Code CSB-E08702r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08424
Lead Time 3-5 working days
Abbreviation Renin
Target Name Renin
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 mU/mL-1000 mU/mL
Sensitivity 3.9 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-99
1:2Average %90
Range %85-94
1:4Average %99
Range %92-104
1:8Average %88
Range %84-92
Recovery
The recovery of rat renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9792-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
10002.497 2.382 2.440 2.254
5001.773 1.641 1.707 1.521
2501.152 1.199 1.176 0.990
1250.693 0.738 0.716 0.530
62.50.479 0.515 0.497 0.311
31.20.318 0.309 0.314 0.128
15.60.234 0.217 0.226 0.040
00.191 0.180 0.186  
References
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Function Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Subcellular Location Secreted, Membrane
Protein Families Peptidase A1 family
Database Links

KEGG: rno:24715

STRING: 10116.ENSRNOP00000003951

UniGene: Rn.9831

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