Pig interleukin 8,IL-8 ELISA Kit

Code CSB-E06787p
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

This Pig IL8 ELISA Kit was designed for the quantitative measurement of Pig IL8 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Target Name interleukin 8
Alternative Names CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
Abbreviation IL8
Uniprot No. P26894
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %91-99
1:2Average %90
Range %86-94
1:4Average %88
Range %84-93
1:8Average %92
Range %88-97
Recovery
The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9995-104
EDTA plasma (n=4)8581-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.662 2.601 2.632 2.460
40002.215 2.161 2.188 2.016
20001.498 1.404 1.451 1.279
10000.871 0.819 0.845 0.673
5000.510 0.507 0.509 0.337
2500.380 0.359 0.370 0.198
1250.271 0.261 0.266 0.094
00.174 0.170 0.172  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Cells

Review: The results are very satisfactory.I used the cell culture medium supernatant, and the other kits had interfering factors, but the CUSABIO did not, and after trying several other kits, I found that this brand was the only one that worked well.

By Anonymous

Target Background

Function
(From Uniprot)
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Gene References into Functions
  1. This study evaluated the subcellular localization and other functions of TGEV nsp7 protein through analysis of its effects on cell growth, cell cycle progression, interleukin 8 (IL-8) expression, and NF-kappaB activation. PMID: 29521102
  2. The JNK-activated AP-1 subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV PMID: 28347884
  3. These results suggest that porcine circovirus 2 induces IL-8 secretion via the TLR2/MyD88/NF-kappaB signalling pathway. PMID: 26581603
  4. Facilitated degradation of the RGS16 by porcine circovirus type 2 ORF3 further enhances NFkappaB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts. PMID: 25575706
  5. Suggest that the interplay between TGFbeta-2 and LPS regulates the levels of IL-8 in the immature newborn intestine. PMID: 25147235
  6. downregulated gene expression elite controllers (fraction of human population displaying resistance to HIV infection) PMID: 23751822
  7. Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
  8. Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
  9. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
  10. In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
  11. Classical swine fever virus NS2 protein promotes interleukin-8 expression. PMID: 21318239
  12. Developmental changes in IL-8 isoforms may serve to minimize its inflammatory effects in the fetus and also provide a mechanism to restore its full activity after birth PMID: 19232500

Show More

Hide All

Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity Alveolar macrophages.
Database Links

KEGG: ssc:396880

STRING: 9823.ENSSSCP00000026976

UniGene: Ssc.658

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1