Pig plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Code CSB-E06534p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig SERPINE1 ELISA Kit was designed for the quantitative measurement of Pig SERPINE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Alternative Names SERPINE1 ELISA Kit; PAI1 ELISA Kit; PLANH1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
Abbreviation SERPINE1
Uniprot No. P79335
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %83-93
1:2Average %100
Range %95-104
1:4Average %90
Range %85-94
1:8Average %95
Range %89-100
Recovery
The recovery of pig PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-97
EDTA plasma (n=4)9890-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.050 2.012 2.031 1.905
101.669 1.676 1.673 1.547
51.397 1.366 1.382 1.256
2.50.996 0.987 0.992 0.866
1.250.668 0.678 0.673 0.547
0.6250.464 0.457 0.461 0.335
0.3120.303 0.299 0.301 0.175
00.125 0.127 0.126
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
Gene References into Functions
  1. In one pig population and in female pigs, SERPINE1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1) was significantly associated with muscling, growth and fat accretion and in male animals with meat quality. PMID: 22497544
Subcellular Location Secreted.
Protein Families Serpin family
Database Links

KEGG: ssc:396945

UniGene: Ssc.9781

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