Rat Interleukin 1β,IL-1β ELISA Kit

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Rat Interleukin 1β,IL-1β ELISA Kit

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Code	CSB-E08055r-IS
Size	96T,5×96T,10×96T
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Alternative Names

Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit

Abbreviation

IL1B

Uniprot No.

Q63264

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	91-105
1:2	Average %	102
	Range %	95-109
1:4	Average %	99
	Range %	92-105
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.826

2.634

2.730

2.569

1000

2.045

1.967

2.006

1.845

500

1.349

1.328

1.339

1.177

250

0.818

0.829

0.824

0.662

125

0.474

0.491

0.482

0.321

62.5

0.344

0.353

0.349

0.187

31.25

0.238

0.244

0.241

0.080

0.157

0.165

0.161

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Naringenin's Neuroprotective Effect on Diazino-Induced Cerebellar Damage in Male Albino Rats, with Modulation of Acetylcholinesterase AA Saati,Brain Sciences,2025

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

Subcellular Location

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.

Protein Families

IL-1 family

Database Links

STRING: 10116.ENSRNOP00000006308

UniGene: Rn.9869

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Related pathways

MAPK signaling pathway Lipids and Inflammation in Atherogenesis NF-kappa B signaling pathway TNF signaling pathway Necroptosis Hematopoietic cell lineage IL-17 signaling pathway Inflammasome Signaling NOD-like receptor signaling pathway Osteoclast differentiation Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Excitatory synapse pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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