Rat apolipoprotein E (Apo-E) ELISA Kit

Code CSB-E09749r
Size 96T,5×96T,10×96T
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Product Details

Target Name
apolipoprotein E
Alternative Names
ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
15.6 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 92  
Range % 87-98  
1:200 Average % 98  
Range % 93-104  
1:400 Average % 84  
Range % 82-88  
1:800 Average % 90  
Range % 85-97  
Recovery
The recovery of rat Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-94  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
1000 0.067 0.065 0.066    
500 0.121 0.125 0.123    
250 0.213 0.204 0.209    
125 0.386 0.355 0.371    
62.5 0.604 0.590 0.597    
31.2 0.811 0.858 0.835    
15.6 1.109 1.116 1.113    
0 2.155 2.214 2.185    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat APOE ELISA Kit was designed for the quantitative measurement of Rat APOE protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Competitive ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 15.6 ng/mL.

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Target Background

Function
(From Uniprot)
APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
Gene References into Functions
  1. Infusion of AngII did not lead to the formation of dissecting abdominal aortic aneurysms or atherosclerosis in ApoE-knockout rats. PMID: 29166645
  2. Results demonstrated that apolipoprotein E4 (ApoE4)exerted direct neurotoxicity and enhanced the neurotoxicity of Abeta1-40 on spatial cognitive function and hippocampal long term potentiation. This may partly elucidate the mechanism by which APOE4 allele exerted negative effects as a major genetic risk factor for developing Alzheimer's disease. PMID: 28356228
  3. renal denervation improves aortic distensibility and attenuated endothelial dysfunction in ApoE(-/-)-rats. PMID: 27277003
  4. The expression of ApoE and S-100 correlated with the progression of focal cerebral contusion. PMID: 26782580
  5. Apolipoprotein E-deficient rats develop atherosclerotic plaques in partially ligated carotid arteries. PMID: 26545012
  6. rats treated with KD (EXP2) showed a significant down-regulated expression of ZnT-3, MT-3, ApoE, clusterin, and ACAT-1 in hippocampus PMID: 25778834
  7. Findings suggest that endogenous apoE generates lower density HDL to produce more lipidated HDL using previously synthesized cholesterol through the interaction with ABCA1 in caveolin-1-rich domain of astrocytes PMID: 24814386
  8. Although rat apolipoprotein E sequence resembles apoE4, an isoform of apoE3, rat apoE displays the biophysical behavior of apoE3. PMID: 23103361
  9. A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma PMID: 22674573
  10. Lower expression of MMP-2 and MMP-9 is associated with the increased expression of apoE in the glomerulus PMID: 22207560
  11. ApoE-containing astrocyte lipoproteins exhibit the most robust interactions with Abeta. PMID: 22138302
  12. Apolipoproteins E3 exerts neurotrophic and synaptogenic effects in dorsal root ganglion cell cultures. PMID: 19414061
  13. LpE and LRP1 promote axonal extension, and suggest that lipids exported to LpE by ABCG1 are important for the enhancement of axonal extension mediated by LpE. PMID: 21040802
  14. ApoE may play a role in lipid rafts or synaptic structural plasticity by the regulation of its levels via dendritic localization and translation. PMID: 20456011
  15. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection. PMID: 19382209
  16. Although ApoE increases arterial wall accumulation of triglyceride-rich particles(TGRPs), it also reduces the penetration of TGRPs into the arterial wall--a novel antiatherogenic property. PMID: 12482838
  17. ApoE mRNA was expressed in astrocytes and macrophages, but not in neurons after focal cerebral ischemia PMID: 12870266
  18. Spatiotemporal changes of apolipoprotein E immunoreactivity and apolipoprotein E mRNA expression after transient middle cerebral artery occlusion in rat brain. PMID: 12898539
  19. The Apolipoprotein (apo) E is a protein involved in both lipid metabolism and neuroprotection and its expression system will be highly useful for probing the ability of rat apoE to mediate food intake in rats. PMID: 15866734
  20. Data indicate that the role of apoE4 in cholesterol homeostasis and apolipoprotein cell association is more pronounced in hippocampal neurons. PMID: 16376010
  21. Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury. PMID: 16497717
  22. 24(S)-hydroxycholesterol induces apoE-mediated efflux of cholesterol in astrocytes via an LXR-controlled pathway PMID: 16524875
  23. ApoE expression is not altered during normal brain aging, but there may be a relationship between ApoE and IL-1beta transcription in the cerebral cortex PMID: 16802110
  24. LRP binds and endocytoses Abeta42 both directly and via apoE but endocytosed Abeta42 is not completely degraded and accumulates in intraneuronal lysosomes PMID: 17012232
  25. A naturally processed C-terminal ApoE peptide, Ep1.B, has anti-atherogenic activity indicating a role for this naturally metabolized peptide in vascular wound healing and lipid homeostasis PMID: 17126342
  26. treatment of primary rat mixed glial cell cultures with IL-1beta induced a significant increase in extracellular apoE protein; treatment of primary astrocyte and mixed glial cell cultures with TNF-alpha significantly reduced extracellular apoE PMID: 18288929
  27. mRNA expression of ApoE in arterial walls was not different between the controls and cerebral aneurysms. PMID: 18360691
  28. Data demonstrate that apoE suppresses food intake via a mechanism enhancing melanocortin signaling in the hypothalamus. PMID: 18559657
  29. The finding that hypothalamic apoE and food intake are positively associated during the normal circadian cycle as well as in the period of restricted feeding suggests that hypothalamic apoE is food-entrained and regulates food intake. PMID: 19362542
  30. Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin. PMID: 19717566

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Subcellular Location
Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
Protein Families
Apolipoprotein A1/A4/E family
Database Links
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