Rat interferon-inducible protein 10,IP-10 ELISA Kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

chemokine (C-X-C motif) ligand 10

Associated names

Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit

Abbreviation

CXCL10

Uniprot No.

P48973

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL.

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	92
	Range %	83-98
1:4	Average %	91
	Range %	80-96
1:8	Average %	87
	Range %	82-94

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	3.284	3.186	3.235	3.153
2000	2.122	1.996	2.059	1.977
1000	1.453	1.375	1.414	1.332
500	0.840	0.816	0.828	0.746
250	0.422	0.401	0.411	0.329
125	0.256	0.244	0.250	0.168
62.5	0.135	0.142	0.138	0.056
0	0.083	0.081	0.082

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This ELISA kit provides quantitative measurement of CXCL10 (IP-10) in rat samples, supporting immunological research into interferon-γ-driven inflammatory signaling. Because CXCL10 is rapidly induced during Th1-polarized immune responses yet circulates at low baseline levels, accurate quantification across both resting and inflamed states demands a carefully calibrated assay.

Detection Range: The 62.5–4000 pg/mL range spans from near-baseline rat serum CXCL10 concentrations to the elevated levels observed during acute viral infection, transplant rejection, or LPS challenge. Tissue homogenates from inflamed organs may require dilution to fall within range, while unstimulated plasma samples can typically be assayed neat.

Sensitivity: At 31.25 pg/mL, the kit detects CXCL10 in low-expression conditions such as early post-infection time points, naïve control animals, or conditioned media from unstimulated macrophage cultures where the chemokine is minimally secreted.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates allows researchers to correlate systemic CXCL10 levels with local tissue expression — critical for studies mapping chemokine gradients that drive CXCR3⁺ T-cell recruitment to target organs.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate longitudinal bleeding protocols in rats, where repeated sampling limits available volume per time point.

This kit is well suited for studies of experimental autoimmune encephalomyelitis, renal allograft rejection, and hepatic ischemia-reperfusion injury in rat models.

Target Background

Function
(From Uniprot)

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.

Gene References into Functions

These observations provide evidence for IP-10 in a regulatory role in cold ischemia-elicited acute allograft rejection and in PRINS lncRNA expression PMID: 29880386
CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043
data support our hypothesis that increased circulating IFN-gamma and glomerular TNF-alpha induce synergistically the production and secretion of CXCL10 by podocytes, attracting activated macrophages into kidney tissue. PMID: 25561167
CXCL10 is a regulator for an increase of opioid-containing macrophages and antinociceptive mediator in inflammatory pain and as a key chemokine regulated by electroacupuncture PMID: 24732949
CXCL10 may be a necessary algogenic molecule, especially in the development of Cancer-induced bone pain PMID: 24337539
CXCL10 is involved in astrocyte migration in amyloid-beta-stimulated hippocampal astrocytes. PMID: 23270423
CXCL10 is a potential candidate for the reduction in myelination in cultures on TnC-astrocytes PMID: 21917786
CXCL10 and CCL5 but not CXCL11 are involved in the pathogenesis of multiple sclerosis. PMID: 20720435
upregulated in endometriosis tissues PMID: 20452033
interferon-inducible protein 10 (IP-10) as ethanol-responsive gene in the fetal rat brain PMID: 12354640
Antibodies to this chemokine increase IFNgamma in BALF of F344 rats following Sendai virus infection. PMID: 12581489
CXCL10-induced elevations in intracellular Ca2+ are associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and activity of hippocampal neurons. PMID: 15465598
IP-10/CXCR3 signaling is associated with the pathogenesis of experimental autoimmune myasthenia gravis in rats. PMID: 15843529
Our study demonstrates a role of IP-10/CXCL10 on T cell recruitment in a rat model of renal endothelial microvascular injury PMID: 16382019
this study shows that IP-10 contributes to the regulation of the expression of SD (slit-diaphram) components not only in the physiologic state but also in pathologic states. PMID: 16382022
Inhibition of CXCR3/IP-10 signaling can be considered as a potential treatment modality for myasthenia gravis. PMID: 19232748

Hide All

Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

In the central nervous system, CXCL10 is predominantly localized to activated neurons. Expressed in both microglia and astrocytes.

Database Links

KEGG: rno:245920

STRING: 10116.ENSRNOP00000003649

UniGene: Rn.10584

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Code	CSB-E08182r-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.