Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.

Target Names

Cxcl10

Uniprot No.

P48973

Research Area

Immunology

Alternative Names

Cxcl10; Inp10; Mob1; Scyb10C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Interferon-inducible protein 10; Protein Mob-1; Small-inducible cytokine B10

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

22-98aa

Complete Sequence

IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP

Mol. Weight

8.7 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CXCL10 (IP-10) is a key interferon-γ–inducible chemokine that drives CXCR3-dependent lymphocyte trafficking, making it central to studies of inflammation, infection, and tumor immunology. This tag-free recombinant rat CXCL10, encompassing the full mature sequence (residues 22–98), demonstrates confirmed chemotactic activity at 10–50 ng/mL in a bioassay using human CXCR3-transfected HEK293 cells, providing a suitable basis for chemotaxis and migration assays, CXCR3 signaling pathway dissection (including MAPK and PI3K/Akt cascades), and in vivo rat models of inflammatory or tumor biology. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell activation readouts, which supports use in sensitive functional assays such as T-cell polarization and proliferation studies. Produced in *E. coli* with greater than 95% purity by SDS-PAGE, this protein also meets the quality thresholds commonly required for use as a reference standard in cytokine detection assays or as a positive control in ELISA and antibody validation workflows.

Target Background

Function

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.

Gene References into Functions

These observations provide evidence for IP-10 in a regulatory role in cold ischemia-elicited acute allograft rejection and in PRINS lncRNA expression PMID: 29880386
CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043
data support our hypothesis that increased circulating IFN-gamma and glomerular TNF-alpha induce synergistically the production and secretion of CXCL10 by podocytes, attracting activated macrophages into kidney tissue. PMID: 25561167
CXCL10 is a regulator for an increase of opioid-containing macrophages and antinociceptive mediator in inflammatory pain and as a key chemokine regulated by electroacupuncture PMID: 24732949
CXCL10 may be a necessary algogenic molecule, especially in the development of Cancer-induced bone pain PMID: 24337539
CXCL10 is involved in astrocyte migration in amyloid-beta-stimulated hippocampal astrocytes. PMID: 23270423
CXCL10 is a potential candidate for the reduction in myelination in cultures on TnC-astrocytes PMID: 21917786
CXCL10 and CCL5 but not CXCL11 are involved in the pathogenesis of multiple sclerosis. PMID: 20720435
upregulated in endometriosis tissues PMID: 20452033
interferon-inducible protein 10 (IP-10) as ethanol-responsive gene in the fetal rat brain PMID: 12354640
Antibodies to this chemokine increase IFNgamma in BALF of F344 rats following Sendai virus infection. PMID: 12581489
CXCL10-induced elevations in intracellular Ca2+ are associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and activity of hippocampal neurons. PMID: 15465598
IP-10/CXCR3 signaling is associated with the pathogenesis of experimental autoimmune myasthenia gravis in rats. PMID: 15843529
Our study demonstrates a role of IP-10/CXCL10 on T cell recruitment in a rat model of renal endothelial microvascular injury PMID: 16382019
this study shows that IP-10 contributes to the regulation of the expression of SD (slit-diaphram) components not only in the physiologic state but also in pathologic states. PMID: 16382022
Inhibition of CXCR3/IP-10 signaling can be considered as a potential treatment modality for myasthenia gravis. PMID: 19232748

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

In the central nervous system, CXCL10 is predominantly localized to activated neurons. Expressed in both microglia and astrocytes.

Database Links

KEGG: rno:245920

STRING: 10116.ENSRNOP00000003649

UniGene: Rn.10584

Code	CSB-AP001451RA
Abbreviation	Recombinant Rat Inp10 protein (Active)
MSDS	MSDS Datasheet
Size	$170
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Recombinant Rat C-X-C motif chemokine 10 protein (Cxcl10) (Active)

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