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Rat interferon-inducible protein 10,IP-10 ELISA Kit

Citations (3) Protocol
Code CSB-E08182r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-X-C motif) ligand 10
Alternative Names Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit
Abbreviation CXCL10
Uniprot No. P48973
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 781.25 pg/mL-50000 pg/mL
Sensitivity 195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %88
Range %84-92
1:2Average %94
Range %90-98
1:4Average %99
Range %95-104
1:8Average %102
Range %96-108
Recovery
The recovery of rat IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
500002.556 2.581 2.569 2.437
250001.748 1.769 1.759 1.627
125001.002 1.085 1.044 0.912
62500.663 0.699 0.681 0.549
31250.396 0.387 0.392 0.260
1562.50.284 0.271 0.278 0.146
781.250.219 0.230 0.225 0.093
00.131 0.133 0.132 　
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.
Gene References into Functions
  1. These observations provide evidence for IP-10 in a regulatory role in cold ischemia-elicited acute allograft rejection and in PRINS lncRNA expression PMID: 29880386
  2. CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043
  3. data support our hypothesis that increased circulating IFN-gamma and glomerular TNF-alpha induce synergistically the production and secretion of CXCL10 by podocytes, attracting activated macrophages into kidney tissue. PMID: 25561167
  4. CXCL10 is a regulator for an increase of opioid-containing macrophages and antinociceptive mediator in inflammatory pain and as a key chemokine regulated by electroacupuncture PMID: 24732949
  5. CXCL10 may be a necessary algogenic molecule, especially in the development of Cancer-induced bone pain PMID: 24337539
  6. CXCL10 is involved in astrocyte migration in amyloid-beta-stimulated hippocampal astrocytes. PMID: 23270423
  7. CXCL10 is a potential candidate for the reduction in myelination in cultures on TnC-astrocytes PMID: 21917786
  8. CXCL10 and CCL5 but not CXCL11 are involved in the pathogenesis of multiple sclerosis. PMID: 20720435
  9. upregulated in endometriosis tissues PMID: 20452033
  10. interferon-inducible protein 10 (IP-10) as ethanol-responsive gene in the fetal rat brain PMID: 12354640
  11. Antibodies to this chemokine increase IFNgamma in BALF of F344 rats following Sendai virus infection. PMID: 12581489
  12. CXCL10-induced elevations in intracellular Ca2+ are associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and activity of hippocampal neurons. PMID: 15465598
  13. IP-10/CXCR3 signaling is associated with the pathogenesis of experimental autoimmune myasthenia gravis in rats. PMID: 15843529
  14. Our study demonstrates a role of IP-10/CXCL10 on T cell recruitment in a rat model of renal endothelial microvascular injury PMID: 16382019
  15. this study shows that IP-10 contributes to the regulation of the expression of SD (slit-diaphram) components not only in the physiologic state but also in pathologic states. PMID: 16382022
  16. Inhibition of CXCR3/IP-10 signaling can be considered as a potential treatment modality for myasthenia gravis. PMID: 19232748

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Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity In the central nervous system, CXCL10 is predominantly localized to activated neurons. Expressed in both microglia and astrocytes.
Database Links

KEGG: rno:245920

STRING: 10116.ENSRNOP00000003649

UniGene: Rn.10584
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