Rat interferon-inducible protein 10,IP-10 ELISA Kit

CloseMessage

Product Details

Citations

Related Products

Target Data

Most popular with customers

CloseMessage

Product Details

Target Name

chemokine (C-X-C motif) ligand 10

Alternative Names

Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit

Abbreviation

CXCL10

Uniprot No.

P48973

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

781.25 pg/mL-50000 pg/mL

Sensitivity

195 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	94
	Range %	90-98
1:4	Average %	99
	Range %	95-104
1:8	Average %	102
	Range %	96-108

Recovery

The recovery of rat IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	100	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50000	2.556	2.581	2.569	2.437
25000	1.748	1.769	1.759	1.627
12500	1.002	1.085	1.044	0.912
6250	0.663	0.699	0.681	0.549
3125	0.396	0.387	0.392	0.260
1562.5	0.284	0.271	0.278	0.146
781.25	0.219	0.230	0.225	0.093
0	0.131	0.133	0.132

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Data

Function	In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.
Gene References into Functions	These observations provide evidence for IP-10 in a regulatory role in cold ischemia-elicited acute allograft rejection and in PRINS lncRNA expression PMID: 29880386 CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043 data support our hypothesis that increased circulating IFN-gamma and glomerular TNF-alpha induce synergistically the production and secretion of CXCL10 by podocytes, attracting activated macrophages into kidney tissue. PMID: 25561167 CXCL10 is a regulator for an increase of opioid-containing macrophages and antinociceptive mediator in inflammatory pain and as a key chemokine regulated by electroacupuncture PMID: 24732949 CXCL10 may be a necessary algogenic molecule, especially in the development of Cancer-induced bone pain PMID: 24337539 CXCL10 is involved in astrocyte migration in amyloid-beta-stimulated hippocampal astrocytes. PMID: 23270423 CXCL10 is a potential candidate for the reduction in myelination in cultures on TnC-astrocytes PMID: 21917786 CXCL10 and CCL5 but not CXCL11 are involved in the pathogenesis of multiple sclerosis. PMID: 20720435 upregulated in endometriosis tissues PMID: 20452033 interferon-inducible protein 10 (IP-10) as ethanol-responsive gene in the fetal rat brain PMID: 12354640 Antibodies to this chemokine increase IFNgamma in BALF of F344 rats following Sendai virus infection. PMID: 12581489 CXCL10-induced elevations in intracellular Ca2+ are associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and activity of hippocampal neurons. PMID: 15465598 IP-10/CXCR3 signaling is associated with the pathogenesis of experimental autoimmune myasthenia gravis in rats. PMID: 15843529 Our study demonstrates a role of IP-10/CXCL10 on T cell recruitment in a rat model of renal endothelial microvascular injury PMID: 16382019 this study shows that IP-10 contributes to the regulation of the expression of SD (slit-diaphram) components not only in the physiologic state but also in pathologic states. PMID: 16382022 Inhibition of CXCR3/IP-10 signaling can be considered as a potential treatment modality for myasthenia gravis. PMID: 19232748 Show More Hide All
Subcellular Location	Secreted
Protein Families	Intercrine alpha (chemokine CxC) family
Tissue Specificity	Spleen.
Database Links	KEGG: rno:245920 STRING: 10116.ENSRNOP00000003649 UniGene: Rn.10584

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-E08182r
Size	96T,5×96T,10×96T
See More Details
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.