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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of rat MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of rat MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||95||90-98|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
CUSABIO's rat CCL2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of CCL2 concentrations in serum, plasma, and tissue homogenates. This assay exclusively recognizes rat CCL2. The quantitative sandwich ELISA technique of this kit is based on CCL2 antibody-CCL2 antigen interactions and an HRP colorimetric detection system to detect the levels of CCL2 in samples. The intensity of the color is positively proportional to the amount of bound CCL2 in the initial step.
CCL2 is the most powerful chemoattractant involved in macrophage recruitment and a potentinitiator of inflammation. It exerts potent chemotactic, stimulatory, and mitogenic effects on mononuclear cells. CCL2 also plays an important role in coordinating the immune response after microbial infection by regulating T cell polarization as well as leukocyte migration and accumulation within infected tissues. CCL2/CCR2 signaling has multiple pro-tumorigenic roles, including mediating tumor growth and angiogenesis as well as recruiting and usurping host stromal cells to support tumor progression. CCL2 upregulation has been linked to tumor metastasis, invasion, and immune resistance.
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