Rat monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

Instructions
Code CSB-E07429r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 2
Alternative Names Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Immediate-early serum-responsive protein JE ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation CCL2
Uniprot No. P14844
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 91-105  
1:2 Average % 95  
Range % 89-98  
1:4 Average % 97  
Range % 92-102  
1:8 Average % 95  
Range % 86-98  
Recovery
The recovery of rat MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 95 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.459 2.445 2.452 2.289  
1000 1.924 1.786 1.855 1.692  
500 1.262 1.251 1.257 1.094  
250 0.767 0.755 0.761 0.598  
125 0.473 0.468 0.471 0.308  
62.5 0.334 0.327 0.331 0.168  
31.25 0.246 0.238 0.242 0.079  
0 0.166 0.160 0.163    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Chemotactic factor that attracts monocytes, but not neutrophils.
Gene References into Functions
  1. Study shows that the unique combination of monocyte chemoattractant protein-1 andsecreted ectodomain of sialic acid-binding Ig-like lectin-9 ameliorates rat acute liver failure by inhibiting hepatocellular apoptosis and promoting liver regeneration through the induction of anti-inflammatory/tissue-repairing M2 macrophages. PMID: 28272428
  2. Light exposure led to apoptosis of photoreceptors, which expressed CCL2, accelerating an inflammation-mediated cascade by activating and attracting microglia and monocytes and promoting their secretion of CCL2 in the injured position. PMID: 29142497
  3. MCP-1/sSiglec-9 regenerates peripheral nerves by inducing tissue-repairing M2 macrophages. PMID: 27862629
  4. Tibial fracture surgery in a rat model promoted CCL2 upregulation in activated astrocytes, increased CCR2 expression in activated microglia, and induced deficits in learning and memory. Site-directed pre-injection of RS504393, a CCR2 antagonist, inhibited this effect by reducing microglial activation, M1 polarization, inflammatory cytokines, and neuronal injury and death and improving cognitive function. PMID: 28587818
  5. Low wall shear stress concomitant with turbulent flow contributes to sustained expression of MCP-1 in endothelial cells and presumably plays a role in facilitating macrophage infiltration and exacerbating inflammation, which leads to enlargement or rupture of intracranial aneurysms. PMID: 27160403
  6. An association between serum MCP-1 and SDF-1 concentrations and behavioral manifestations of neuropathic pain. PMID: 26974138
  7. The increase of MCP-1 accompanying the development of hypoxic pulmonary hypertension probably originates from other sources than alveolar macrophages. PMID: 27429111
  8. Dexmedetomidine is a potent suppressor of MCP-1 in astrocytes induced with lipopolysaccharide through alpha2A-adrenergic receptors, which potentially explains its beneficial effects in the treatment of delirium by attenuating neuroinflammation. PMID: 28286770
  9. This study showed that CCL2, NF-kappaB1, RAC2, FCER1G and C1Q may contribute to the generation of neuropathic pain after sciatic nerve injury via immune and defense pathways. PMID: 28033741
  10. Results demonstrate that prenatal high-fat diet exposure greatly disturbs the functioning of the CCL2 chemokine system in embryonic hypothalamic neurons, reducing its endogenous levels and ability to promote the migration of neurons and their expression of orexigenic peptides PMID: 26979053
  11. These results demonstrate that high glucose enhances MCs proliferation and MCP-1 expression by activating the ROS/NF-kappaB pathway and can be inhibited by EE. Our findings provide a new perspective for the clinical treatment of diabetic nephropathy . PMID: 26644089
  12. CCL2 mRNA is increased in adolescent brain, but not adult brain during withdrawal from chronic alcohol. PMID: 26556523
  13. Our study showed that hyperuricemia accelerates renal progression via a mechanism linked to high MCP-1 which may mediate the inflammation reaction of renal diseases induced by hyperuricemia. PMID: 25830624
  14. Report a dual effect of n-3 PUFAs on the regulation of Mcp-1 expression in vascular smooth muscle. PMID: 26402697
  15. Study identifies an innate neuronal TLR4 signal that functions in central nucleus of the amygdala and ventral tegmental area to regulate the initiation of excessive alcohol drinking through MCP-1 neurotransmission likely at the level of dopamine PMID: 25567426
  16. The expressions of MCP-1 proteins began to increase gradually after balloon injury and were obviously detected in the VSMC on the 4th and 7th day, until 14th day when it was still detected clearly in the proliferating intima. PMID: 26062549
  17. TGFalpha signaling stimulates cartilage degradation via a CCL2-dependent mechanism, but pharmacologic inhibition of the TGFalpha-CCL2 axis reduces experimental posttraumatic osteoarthritis progression in vivo. PMID: 26138996
  18. Inhibition of monocyte chemoattractant peptide-1 decreases secondary spinal cord injury PMID: 25672988
  19. Stromal cell-derived CCL2 (in contrast to CCL2 immune cell-derived) tightly controls monocyte/macrophage recruitment into the lesion and plays a major role in the development of neuropathic pain. PMID: 25449579
  20. Inhibition of S1PR2 down-regulate the expressions of SphK1 and MCP-1 in glomerular mesangial cells under high glucose. PMID: 25575056
  21. findings suggest that the LPS-miR-34a-CCL22 axis may contribute to the recruitment of Tregs in periapical lesions, providing a potential therapeutic target for controlling this disease PMID: 25817785
  22. role of CCL2 in monocyte trafficking into the retina in diabetic retinopathy PMID: 25329075
  23. aging can reduce the expression of CINC and MCP-1 mRNA in lung tissues, and reduce the infiltration of neutrophils and monocyte-macrophages induced by CINC and MCP-1. This might lead to increased risk of pneumonia in elderly patients. PMID: 24464584
  24. Serum concentration of chemokine (c-c motif) ligand 2, L-selectin and intra cellular adhesion molecule-1 also increased with growing tumor PMID: 25667449
  25. PKC-NF-kappaB are involved in CCL2-induced elevation of Nav1.8 current density by promoting the phosphorylation of Nav1.8 and its expression. PMID: 24724624
  26. IgE alone is sufficient to stimulate basophils to increase expression of MCP-1, which in turn might contribute to the inflammatory response. PMID: 24980870
  27. Results suggest that CCL2/CCR2 in the dorsal root ganglion and spinal cord is involved in the maintenance of lumbar disc herniation-induced pain PMID: 24462503
  28. MCP-1 induces persistent muscle hyperalgesia and latent chronic sensitization. PMID: 24637038
  29. This study indicates that TNF-alpha was released from the activated microglia induced by retinal neuronal MCP-1 via the p38, ERK, and NF-kappaB pathways, but not c-Jun N-terminal kinase. PMID: 24826069
  30. Epithelium-derived exosomal ATF3 RNA attenuates ischemia-reperfusion induced kidney injury by inhibiting MCP-1 gene transcription. PMID: 24420912
  31. Hesperetin-7-O-glucuronide decreased hydrogen peroxide-induced intracellular adhesion molecule-1 and monocyte chemoattractant protein-1 mRNA expression in rat aortic endothelial cells. PMID: 23831969
  32. osteoblast and osteocyte MCP-1 expression is an important mediator for the anabolic effects of PTH on bone. PMID: 23519994
  33. TGF-beta1 exhibits pro-inflammatory effects through the upregulation of MCP-1 in peritoneal fibrosis. Smad7 inhibits TGF-beta1 induced MCP-1 upregulation through a MAPK/p38-dependent pathway. PMID: 23242502
  34. An increase in dentate MCP-1 is associated with seizure-induced aberrant cell migration of neuronal progenitor cells. PMID: 23339567
  35. These data support an important role for MCP-1 in the early phase of stress fracture repair and activated remodeling PMID: 23460341
  36. The present study suggests that the expressed on endothelial cells and astrocytes are responsible for the recruitment of brain Iba1(+) /NG2(+) cells progenitors during the acute phase of stroke. PMID: 23400803
  37. Report functional roles for CCL2/CCR2 signaling at the level of the urinary bladder in reducing voiding frequency and somatic sensitivity following CYP-induced cystitis. PMID: 23594826
  38. MCP-1 and other bone matrix proteins might be involved in the formation of vascular lesions of diabetic nephropathy. PMID: 22812249
  39. glucocorticoid-mediated repression of IL-1beta-induced CCL2 gene transcription and protein secretion occurs in part through the upregulation of the MKP-1 gene PMID: 23056550
  40. High-dose losartan can effectively control blood pressure, reduce renal damage and fibrosis, and inhibit MCP1 and TGF-beta(1) expression in rats with unilateral ureteral obstruction. PMID: 21868333
  41. These results indicated that the increase in urinary CCL2 is associated with tubular injury and serves as a sensitive and noninvasive marker for the early detection of cisplatin-induced tubular injury. PMID: 23291264
  42. spinal neuronal MCP-1 is a key mediator of the spinal microglial activation and is involved in morphine antinociceptive tolerance PMID: 23187120
  43. MCP-1 upregulation in IC/BPS is one of possible contributing factors inducing histamine release from mast cells . PMID: 23049171
  44. Peptide fragment 29-40 of amino acid sequence of monocyte chemoattractant protein-1 (MCP-1) stimulates monocyte migration in vivo and facilitates wound healing. PMID: 23129286
  45. Xiongshao capsule could enhance the protective effect of ischemic postconditioning by inhibiting MCP-1 and TNF-alpha in rats with myocardial reperfusion injury. PMID: 21302491
  46. Upregulation of MCP-1 began during the early stage of DR and increased with the development of the disease. PMID: 23010641
  47. CCL2-CCR2 signaling cascades play a role in neuron-glia-cytokine interactions and the descending facilitation of neuropathic pain. PMID: 22466130
  48. These studies define new roles for glucocorticoid receptor, Y-box binding protein 1, and RNase UK114 in the formation of a molecular complex that degrades MCP-1 mRNA. PMID: 22801372
  49. MCP-1 through CCR2 was implicated in Angiotensin II-stimulated proliferation of vascular smooth muscle cells PMID: 22527942
  50. Altered pulmonary gene expression of PPARgamma and MCP-1 during late gestation may impair lung development and maturation, contributing to pulmonary hypoplasia in the nitrofen-induced congenital diaphragmatic hernia model. PMID: 22595559

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Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: rno:24770

STRING: 10116.ENSRNOP00000009448

UniGene: Rn.4772

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