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| Code | CSB-E05126r |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The Human RANKL ELISA Kit allows for the in vitro quantitative determination of RANKL concentrations in serum, plasma, or cell culture supernates. This kit exclusively recognizes human RANKL. RANKL, also called TNFSF11, is expressed by mesenchymal cells of the bone regulating osteoclast differentiation and bone remodeling. It plays an important role in bone homeostasis and lymphoid tissue formation. The RANKL-RANK-OPG system is renowned for its roles in osteoclasts maturation as well as bone modeling and remodeling. RANKL-RANK signaling activates multiple downstream signaling pathways required for osteoclast development and formation, including MAPK-p38-JNK and NFκB pathways. OPG shows a higher binding affinity to RANKL than RANK and thus blocks RANKL from ligating to RANK suppressing osteoclastogenesis and protecting the bone from excessive osteoclast-mediated resorption. The detection mechanism of this kit is based on the Sandwich-ELISA technique. RANKL in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled RANKL antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with RANKL content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency. |
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| Target Name | tumor necrosis factor (ligand) superfamily, member 11 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative Names | Tnfsf11 ELISA Kit; Opgl ELISA Kit; Rankl ELISA Kit; Trance ELISA Kit; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; Osteoclast differentiation factor ELISA Kit; ODF ELISA Kit; Osteoprotegerin ligand ELISA Kit; OPGL ELISA Kit; Receptor activator of nuclear factor kappa-B ligand ELISA Kit; RANKL ELISA Kit; TNF-related activation-induced cytokine ELISA Kit; TRANCE ELISA Kit; CD antigen CD254) [Cleaved into: Tumor necrosis factor ligand superfamily member 11 ELISA Kit; membrane form; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; soluble form] ELISA Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abbreviation | TNFSF11 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Uniprot No. | Q9ESE2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species | Rattus norvegicus (Rat) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample Types | serum, plasma, cell culture supernates | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Range | 62.5 pg/mL-4000 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 15.6 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Time | 1-5h | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample Volume | 50-100ul | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Wavelength | 450 nm | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Research Area | Cardiovascular |
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| Assay Principle | quantitative | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Measurement | Sandwich | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Precision |
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| Linearity |
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| Recovery |
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| Typical Data |
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Troubleshooting and FAQs |
ELISA kit FAQs | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Storage | Store at 2-8°C. Please refer to protocol. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lead Time | 3-5 working days | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Function (From Uniprot) |
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
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| Gene References into Functions |
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| Subcellular Location | Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor ligand superfamily member 11, soluble form]: Secreted. |
| Protein Families | Tumor necrosis factor family |
| Tissue Specificity | Highly expressed in thymus and bone tissues. |
| Database Links |
KEGG: rno:117516 STRING: 10116.ENSRNOP00000013210 UniGene: Rn.217570 |
