The Rat vascular cell adhesion protein 1 (VCAM1/CD106) ELISA Kit allows for the in vitro quantitative determination of VCAM1concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes rat VCAM1. The kit has been quality controlled with high sensitivity, strong specificity, good linearity, precision less than 10%, recovery with 85%-104%, and high lot-to-lot consistency. Refer to the product manual for more information.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. VCAM1 in the sample is bound to the anti-rat VCAM1antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled VCAM1 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with VCAM1 content in the sample.
VCAM1, also named CD106, is a glycoprotein expressed mainly in endothelial cells upon activation by extracellular stimuli, including reactive oxygen species and pro-inflammatory cytokines, such as TNFα and IL-1. Under an inflammatory condition, VCAM1 directly interacts with α4β1 integrin expressed on leukocytes, mediating leukocyte adhesion and transendothelial migration across endothelial cells. Recent evidence suggests that VCAM1 is closely related to the progression of various immunological disorders, including rheumatoid arthritis, asthma, transplant rejection, and cancer.