Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Vcam1

Uniprot No.

P29534

Research Area

Others

Alternative Names

Vcam1; Vcam-1; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; CD antigen CD106

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

31-697aa

Target Protein Sequence

PEYKTLAQIGDSMLLTCSTTGCESPSFSWRTQIDSPLNGKVKTEGAKSVLTMDPVSFENEHSYLCTATCNSGKLERGIQVDIYSFPKDPEIQFSGPLEVGKPVMVKCLAPDVYPIDRLEIELFKGDRLMKKQDFVDEMAKKSLETKSLEVIFTPVIEDIEKALVCRAKLYIDQTDSIPKERETVRELQVYTSPKNTEISVHPSTRLHEGAAVTMTCASEGLPAPEIFWSKKLDNGVLQLLSGNATLTLIAMRMEDSGIYVCEGVNLVGRDKTEVELIVQEKPFTVDISPGSQVAAQVGDSVVLTCAAVGCDSPSFSWRTQTDSPLNGEVRDEGATSTLTLSPVGVEDEHSYLCTVTCQRRKLEKTIQVEVYSFPEDPEIEISGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLLNKFLREEIGTKSLETKSLEMTFIPTAEDTGKALVCLAKLHSSQMESEPKQRQSTQTLYVNVAPKEPTIWVSPSPVPEEGSPVNLTCSSDGFPTPKILWSRQLKNGELQPLSQNTTLSFMATKMEDSGIYVCEGINEAGISKKSVELIIQGSSKDIQLTVFPSKSVKEGDTVIISCTCGSVPEIWIILKKKAKTGDMVLKSVNGSYTIRKAQLQDAGVYECESKTEVGSQLRSLTLDVKGKENNKDYFSP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

77.4kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombination of a plasmid encoding the Rat Vcam1 protein (31-697aa) is the first step during the production the recombinant Rat Vcam1 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 6xHis tag. After expression, affinity purification is used to isolate and purify the recombinant Rat Vcam1 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Rat Vcam1 protein. Its purity exceeds 90%.

Target Background

Function

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Gene References into Functions

Western blotting analyses validated the fact that miR-142-5p inhibited the expression of VCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. PMID: 29789783
Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway. PMID: 27620662
Berberine exhibited renoprotective effects in diabetic nephropathy (DN), and the underlying molecular mechanisms may be associated with changes in the levels and regulation of beta-arrestin 1/2 expression, as well as intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) levels in the kidney. PMID: 26531813
The combination of DC vaccination and antiangionesis inhibited the rats with malignant glioma by stimulating immune response, supressing the expression of angiogenesis-related proteins VEGF, VCAM, and ICAM-1 PMID: 27072237
VCAM1 was strikingly increased in neurons after intracerebral hemorrhage. PMID: 25868755
Activation of Wnt/beta-catenin signaling in AECs attenuated intercellular adhesion molecule 1 (ICAM-1)/vascular cell adhesion molecule 1 (VCAM-1)-mediated adhesion of both macrophages and neutrophils to AECs. PMID: 26351298
Adjuvant-induced arthritis was not associated with a change in VCAM1 levels. PMID: 25708190
Expression of E-selectin and CAMs was also modified in an age- and ischemia/reperfusion-dependent manner. PMID: 25182537
Upregulation of the adhesion molecules VCAM-1, ICAM-1, and E-selectin. PMID: 24289084
It is reasonable to conclude that PDGFBB significantly enhanced the expression of VCAM-1 in BM-MSCs, which facilitated the migration of BM-MSCs towards PDGFBB. PMID: 24100802
These findings suggested that glioma-induced change in VCAM-1 expression of bone marrow stem cells may play an important role in their tropism towards glioma. PMID: 22562815
Pretreatment of LPS-stimulated vascular endothelial cells with berberine dose-dependently decreased the number of adhered leukocytes and VCAM-1 expression at both RNA and protein levels. PMID: 23058024
results indicate that endothelin-1 stimulates arterial VCAM-1 expression through p38MAPK-dependent activation of neutral sphingomyelinases PMID: 22627111
Clerodendron glandulosum.Coleb leaf extract attenuates in vitro macrophage differentiation and expression of VCAM-1 and P-selectin in thoracic aorta of atherogenic diet fed rats. PMID: 21961520
A cholesterol diet does not play a role in the expression f VCAM-1 in the rat aorta. PMID: 22351665
VCAM1 is expressed at different levels in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs. PMID: 21604443
Report specific adhesion and accumulation of VCAM-1-targeted ultrasound microbubbles to inflammatory endothelial cells under hemodynamic shear flow simulation. PMID: 22195873
black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expressions through blockage of NF-kappaB and JNK activation in intestinal epithelial cells PMID: 21184129
The expression of ICAM-1 and VCAM-1 was significantly decreased in the kidney of spontaneously hypertensive rats treated with Cordceps Sinensis. PMID: 20197615
Tanshinone IIA treatment leads to downregulation of ICAM-1 and VCAM-1 protein expression in brain microvascular endothelial cells. PMID: 20687137
Isoflurane, sevoflurane and desflurane down-regulated the expression of VCAM-1 in a dose-dependent manner. PMID: 20936719
Data indicates single-walled carbon nanotube exposure induces oxidative stress, thereby altering ICAM-1 and VCAM-1 expression. PMID: 21121367
Puerarin inhibits the expression of VCAM and P-selectin in the aorta of diabetic rats. PMID: 18095572
Fenofibrate can effectively prevent atherosclerosis by restoring NO concentration and down-regulating VCAM-1 expression. PMID: 18159007
Gene expressions of LOX-1 and VCAM-1 were higher in the aortic arch of HIV-1transgenic rats (Tg); the level of sICAM-1 was elevated in the HIV-1Tg rats but the ICAM-1 gene expression profile did not show any differences. PMID: 19997643
VCAM-1(+) immunoreactivity indicate an important role of choroid plexus in the induction of acute experimental autoimmune encephalomyelitis PMID: 19922364
Integrin alpha2beta1 on rat myeloma cells modulates interaction of alpha4beta1 integrin with vascular cell adhesion molecule-1 but not fibronectin. PMID: 12160302
During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of VCAM-1. PMID: 12196270
iNOS and VCAM-1 are induced by angiotensin II in a pathway that involves p38 MAPK PMID: 15001568
Results are consistent with a role for the integrin alpha4beta1--vascular cell adhesion molecule-1 interaction in extension of sympathetic axons into the myocardium. PMID: 15366013
Suppression of the expression levels of VCAM-1 is associated with the amelioration of chronic rejection by various drugs, suggesting that these molecules are important key molecules in chronic rejection. PMID: 15919506
clusters of VCAM-1(+) immunoreactive cells localized in the choroid plexus epithelium and hypothalamo-hypophyses portal vessel system indicate an inflammation in acute autoimmune encephalitis PMID: 17064783
These results suggest that P2Y2R activation in salivary gland cells increases the EGFR-dependent expression of VCAM-1 and the binding of lymphocytes. PMID: 17599409
candidate genes for salt-sensitive hypertension in the SHRSP, including Edg1 and Vcam1, which are differentially expressed and lie on common and functionally important pathways. PMID: 17938382
Thromboxane receptor upregulates IL-1beta-induced vascular cell adhesion molecule 1 (VCAM-1) expression by enhancing the activation of c-Jun N-terminal kinase (JNK) pathway PMID: 18032781
the expression of VCAM-1 protein was upregulated in cerebral and common carotid arteries of simulated microgravity rats, and the upregulation of VCAM-1 may contribute to impaired endothelium-dependent relaxation in simulated microgravity rat vasculature PMID: 18221587
VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery. PMID: 19958991

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in aortic endothelial cells, with low expression in the descending thoracic aorta and the outer curvature of the aortic arch, where pulsatory shear stress exists, and high in the inner curvature of the aortic arch, where oscillatory shear stress

Database Links

KEGG: rno:25361

STRING: 10116.ENSRNOP00000019377

UniGene: Rn.11267

Code	CSB-EP025809RA1
Abbreviation	Recombinant Rat Vcam1 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP167294r could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Vcam1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP167294r could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Vcam1.
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Recombinant Rat Vascular cell adhesion protein 1 (Vcam1), partial

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