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Sheep Interleukin-8,IL-8 ELISA Kit

Citations (3) Protocol MSDS
Code CSB-E13202Sh
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 8
Alternative Names
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
Abbreviation
CXCL8
Uniprot No.
P36925
Species
Ovis aries (Sheep)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %88
Range %80-95
1:4Average %94
Range %87-99
1:8Average %97
Range %90-105
Recovery
The recovery of sheep IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-100
EDTA plasma (n=4)9691-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.312 2.231 2.272 2.094
2001.753 1.854 1.804 1.626
1001.289 1.339 1.314 1.136
500.824 0.887 0.856 0.678
250.558 0.597 0.578 0.400
12.50.492 0.472 0.482 0.304
6.250.308 0.297 0.303 0.125
00.184 0.171 0.178 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Sheep IL8 ELISA Kit was designed for the quantitative measurement of Sheep IL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Citations

Related Products

CXCL8 Antibodies

CXCL8 Proteins

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Target Background

Function
(From Uniprot)
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Gene References into Functions
  1. In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID: 26342452
  2. IL-8 signaling does not mediate lipopolysaccharide-induced lung inflammation and lung maturation. PMID: 19574422
Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links

KEGG: oas:443418

UniGene: Oar.485

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