Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Instructions
Code CSB-E06994Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name insulin-like growth factor 1 (somatomedin C)
Alternative Names IGF1A, IGFI, OTTHUMP00000195084|insulin-like growth factor 1
Abbreviation IGF1
Uniprot No. Q95222
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 80-100  
1:2 Average % 100  
Range % 91-105  
1:4 Average % 98  
Range % 92-115  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 90 85-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.715 2.725 2.720 2.605  
5 2.035 2.002 2.019 1.904  
2.5 0.989 1.078 1.034 0.919  
1.25 0.591 0.602 0.597 0.482  
0.625 0.342 0.352 0.347 0.232  
0.312 0.201 0.212 0.207 0.092  
0.156 0.175 0.185 0.180 0.065  
0 0.111 0.119 0.115    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: ocu:100008668

STRING: 9986.ENSOCUP00000021389

UniGene: Ocu.2125

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