Rabbit Interleukin 8,IL-8 ELISA Kit

Code CSB-E06905Rb
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 8
Alternative Names
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit
Abbreviation
CXCL8
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %101
Range %94-105
1:2Average %97
Range %91-102
1:4Average %89
Range %85-94
1:8Average %90
Range %86-98
Recovery
The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-96
EDTA plasma (n=4)9289-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.390 2.345 2.368 2.285
7501.587 1.476 1.532 1.449
3750.814 0.778 0.796 0.713
187.50.409 0.389 0.399 0.316
940.276 0.264 0.270 0.187
470.182 0.172 0.177 0.094
23.50.134 0.131 0.133 0.050
00.085 0.081 0.083  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit IL8 ELISA Kit was designed for the quantitative measurement of Rabbit IL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Gene References into Functions
  1. At the 2-wk time point, interleukin 8 gene expression was comparable in intervertebral discs injected with chondrocytes in controls, whereas its expression in IVDs injected with saline increased 50-fold PMID: 25133623
  2. Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
  3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
  4. IL-8 and IL-1ra showed correlation with the severity of colitis. These observations should significantly further understandings on the role of neutrophils and monocytes in the immunopathogenesis of ulcerative colitis PMID: 21807533
Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links
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