CD14 Monoclonal Antibody

Datasheet
Code CSB-MA004879A1m
See More Details Free Antibody trial simple
Size US$350
Image
  • Western Blot
    Positive WB detected in: Rabbit lung tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit small intestine tissue
    All lanes: CD14 antibody at 1:2500
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 41 kDa
    Observed band size: 55 kDa

  • Western Blot
    Positive WB detected in: Mouse kidney tissue, Rat liver tissue
    All lanes: CD14 antibody at 1:2000
    Secondary

    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 41 kDa
    Observed band size: 55 kDa

     

  • Western Blot
    Positive WB detected in: NIH/3T3 whole cell lysate
    All lanes: CD14 antibody at at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 41 kDa
    Observed band size: 55 kDa

  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate
    All lanes: CD14 antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 41 kDa
    Observed band size: 55 kDa

  • IHC image of CSB-MA004879A1m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-MA004879A1m diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-MA004879A1m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-MA004879A1m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of HepG2 cells with CSB-MA004879A1m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of Raw264.7 cells with CSB-MA004879A1m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Product Details

Full Product Name Mouse anti-Human CD14 Monoclonal antibody
Uniprot No. P08571
Target Names CD14
Alternative Names Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form], CD14
Raised in Mouse
Species Reactivity Human, Mouse, Rat, Rabbit
Immunogen Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Purification Method >95%, Protein A purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:64000
IHC 1:50-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Coreceptor for bacterial lipopolysaccharide
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Secreted, Membrane raft, Golgi apparatus
Tissue Specificity Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Database Links

HGNC: 1628

OMIM: 158120

KEGG: hsa:929

STRING: 9606.ENSP00000304236

UniGene: Hs.163867

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.