Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD44 at 2 μg/ml can bind Anti-CD44 mouse monoclonal antibody(CSB-MA004938A0m, antigen from E.coli), the EC50 of the CD44 protein is 11.89-14.94 ng/ml.
Alternative Names
LHR; BA-1; CD 44; CD44; CD44 antigen; CD44 molecule (Indian blood group); CD44 molecule; CD44_HUMAN; CDw44; CDW44 antigen; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; HUTCHI; Hyaluronate receptor; IN; INLU-related p80 Glycoprotein; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH I; MUTCH1; PGP-1; PGP-I; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; Soluble CD44
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
21-220aa
Target Protein Sequence
QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIP
Tag Info
C-terminal hFc-tagged
Form
Liquid or Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Human CD44 Antigen (CD44) is produced in mammalian cells. The CD44 protein (21-220AA) in which a part extracellular domain was encoded by its DNA sequence and this protein carried a human Fc fragment at the C-terminus. CD44 is a surface glycoprotein that plays a part in cell-to-cell interactions, cell proliferation, angiogenesis, and many other cellular activities.
The Recombinant CD44 antigen finds uses in studies on different forms of cancer. This antigen’s role in cellular proliferation, invadopodia formation, cellular survival, and metastasis is of importance in studying different forms of cancer.
By acting on ligands such as hyaluronan, osteopontin, and collagen, CD44 activates various cancer-related pathways. Of such pathways are the NF-kB pathway, RhoGTPases pathway, and the PI3K/AKT pathway. The Recombinant Human CD44 has a molecular weight of 51 kDa and purity over 85% on SDS-PAGE. Its functionality is ELISA-validated and its identity is verified with LC-MS/MS Analysis.