|Request a Quote or Start an on-line Chat
24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%
|Three samples of known concentration were tested twenty times on one plate to assess.
|Inter-assay Precision (Precision between assays): CV%<10%
|Three samples of known concentration were tested in twenty assays to assess.
|To assess the linearity of the assay, samples were spiked with high concentrations of human CD44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
|The recovery of human CD44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
|Average % Recovery
|EDTA plasma (n=4)
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The product CSB-E11846h is a sandwich ELISA kit developed to measure concentrations of human CD44 in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CD44 in samples. The color intensity is measured at 450 nm via a microplate reader.
CD44 is a cell surface adhesion molecule overexpressed on cancer stem cells. Upon binding to its ligand hyaluronic acid (HA), CD44 is activated leading to the activation of cell signaling pathways that induce proliferation, increase cell survival, regulate cytoskeletal alterations, and enhance cellular motility. The interaction of CD44 with hyaluronan also contributes to tumor development, metastasis, and expression of the chemoresistant phenotype. In addition to hyaluronan uptake and degradation, CD44 also participates in angiogenesis, wound healing, as well as tissue formation and patterning. CD44 variants (CD44v) are up-regulated in neoplasia and involved in tumor metastasis and aggression.
There are currently no reviews for this product.