CD44 antigen is a cell-surface glycoprotein in humans that is encoded by CD44 gene. CD44 is expressed in a large number of mammalian cell types. The standard isoform, designated CD44s, comprising exons 1–5 and 16–20 is expressed in most cell types. CD44 splice variants containing variable exons are designated CD44v. Some epithelial cells also express a larger isoform (CD44E), which includes exons v8–10.
CD44 is a transmembrane molecule composed of four functional domains, including N-terminal domain, stem region, transmembrane domain and cytoplasmatic domain. The N-terminal extracellular domain mainly binds various ligands, involving hyaluronic acid (HA), extracellular matrix (ECM) glycoproteins and proteoglycans, growth factors, cytokines and matrix metalloproteinases.
In result of the proteolytic cleavage within the stem region, the extracellular domain is released into the extracellular space. The transmembrane domain anchors and stabilises the molecule in the plasma membrane. The cytoplasmic domain is responsible for signal transduction through binding to different molecules, including cytoskeleton components, kinases and activators of small Rho GTPases (GEFs-guanine nucleotide exchange factors).
CD44 mainly functions as a receptor for HA. It mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression.
In cancer cells, CD44 may plays an important role in invadopodia formation. Moreover, it is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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Hot products of CD44:
● CD44 Monoclonal Antibody (ELISA, WB, IHC, IF, FC) (CSB-MA004938A0m)
Validated Data
Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate
Observed band size: 95 kDa
IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.
IF staining of Hela cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI.
Overlay histogram showing Hela cells stained with CSB-MA004938A0m (red line) at 1:200.
● Recombinant Human CD44 antigen (CD44),partial (Active) (CSB-MP004938HU(F1))
Validated Data
Greater than 90% as determined by SDS-PAGE.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP004938HU(F1) could indicate that this peptide derived from Mammalian Cell-expressed Human CD44.
Measured by its binding ability in a functional ELISA. Immobilized CD44 at 2 μg/ml can bind Anti-CD44 mouse monoclonal antibody(CSB-MA004938A0m), the EC50 of the CD44 protein is 11.89-14.94 ng/ml.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP004938HU(F1) could indicate that this peptide derived from Mammalian Cell-expressed Human CD44.
● Recombinant Human CD44 antigen(CD44),partial,Biotinylated (Active) (CSB-MP004938HU(F1)j1-B)
Validated Data
Greater than 92% as determined by SDS-PAGE.
Immobilized Anti-CD44 Mouse Monoclonal Antibody (CSB-MA004938A0m) at 2 μg/ml can bind Biotinylated human CD44, the EC50 is 2.865-5.099 ng/ml.
The following CD44 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD44 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004938GA01HU | CD44 Antibody | Human,Mouse | ELISA,WB,IHC,IF |
CSB-PA644696 | CD44 Antibody | Human,Rat | ELISA,WB,IF |
CSB-PA135522 | Phospho-CD44 (Ser706) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA001460 | CD44 Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA080035 | Phospho-CD44 (S706) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA282285 | CD44 Antibody | Human,Mouse | ELISA,WB,IHC |
CSB-PA685145 | CD44 Antibody | Human | ELISA,WB |
CSB-PA191560 | CD44 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-RA004938A0HU | CD44 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC |
CSB-PA004938ZA01HU | CD44 Antibody | Homo sapiens | ELISA, WB (ensure identification of antigen) |
CSB-PA004938ZA01HU | CD44 Antibody | Homo sapiens | ELISA, WB (ensure identification of antigen) |
CSB-MA004938A0m | CD44 Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
CSB-MA247417 | CD44 Monoclonal Antibody | Human | ELISA, IHC |
CSB-RA292372A0HU | CD44 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
CSB-RA004938MA1HU | CD44 Recombinant Monoclonal Antibody | Human | ELISA, FC |
CD44 Proteins for Homo sapiens (Human)
CD44 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP004938RA1 CSB-EP004938RA1 CSB-BP004938RA1 CSB-MP004938RA1 CSB-EP004938RA1-B |
Recombinant Rat CD44 antigen (Cd44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF004938RA | Recombinant Rat CD44 antigen (Cd44) | in vitro E.coli expression system |
CD44 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-YP635685DO CSB-EP635685DO CSB-BP635685DO CSB-MP635685DO CSB-EP635685DO-B |
Recombinant Dog CD44 antigen (CD44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD44 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP643679BO CSB-EP643679BO CSB-BP643679BO CSB-MP643679BO CSB-EP643679BO-B |
Recombinant Bovine CD44 antigen (CD44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD44 Proteins for Mesocricetus auratus (Golden hamster)
Code | Product Name | Source |
---|---|---|
CSB-YP723318MRG CSB-EP723318MRG CSB-BP723318MRG CSB-MP723318MRG CSB-EP723318MRG-B |
Recombinant Mesocricetus auratus CD44 antigen (CD44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD44 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Code | Product Name | Source |
---|---|---|
CSB-CF004938DXU | Recombinant Cricetulus griseus CD44 antigen (CD44) | in vitro E.coli expression system |
CSB-YP004938DXU1 CSB-EP004938DXU1 CSB-BP004938DXU1 CSB-MP004938DXU1 CSB-EP004938DXU1-B |
Recombinant Cricetulus griseus CD44 antigen (CD44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD44 Proteins for Papio hamadryas (Hamadryas baboon)
Code | Product Name | Source |
---|---|---|
CSB-CF004938ERJ | Recombinant Papio hamadryas CD44 antigen (CD44) | in vitro E.coli expression system |
CSB-YP004938ERJ1 CSB-EP004938ERJ1 CSB-BP004938ERJ1 CSB-MP004938ERJ1 CSB-EP004938ERJ1-B |
Recombinant Papio hamadryas CD44 antigen (CD44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD44 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF004938MO | Recombinant Mouse CD44 antigen (Cd44) | in vitro E.coli expression system |
CSB-YP004938MO1 CSB-EP004938MO1 CSB-BP004938MO1 CSB-MP004938MO1 CSB-EP004938MO1-B |
Recombinant Mouse CD44 antigen (Cd44), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD44 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E11846h | Human cluster Of differentiation,CD44 ELISA Kit | serum, plasma, cell culture supernates, tissue homogenates | 19.5 pg/mL |