Recombinant Human Cathepsin B(CTSB),partial (Active)

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Code CSB-AP005381HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Specific activity as determined by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC is greater than 3000 pmol/min/ug
Target Names CTSB
Uniprot No. P07858
Research Area Cancer
Alternative Names Amyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CathepsinB; CPSB; CTSB; cysteine protease; OTTHUMP00000116009; OTTHUMP00000229510; OTTHUMP00000229511; OTTHUMP00000229512; OTTHUMP00000229514; OTTHUMP00000229515; OTTHUMP00000229516; Preprocathepsin B
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 18-339aa
Mol. Weight 36.9 kDa
Protein Length Partial
Tag Info C-terminal 6xHis-tagged
Form Liquid
Buffer 0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Gene References into Functions
  1. Cathepsin B (CTSB) is a novel target gene of hypoxia-inducible factor-1-alpha (HIF-1alpha). CTSB mRNA and protein levels can be up-regulated in a HIF-1alpha-dependent manner. PMID: 29935187
  2. expression of cathepsin B and X was detected in stromal cells and cancer cells throughout the glioblastoma (GBM) sections, whereas cathepsin K expression was more restricted to arteriole-rich regions in the GBM sections. Metabolic mapping showed that cathepsin B, but not cathepsin K is active in GSC niches. PMID: 30046941
  3. we evaluated the involvement of cathepsin B and X in the TGF-b1 signaling pathway, one of the key signaling mechanisms triggering epithelial-mesenchymal transition in cancer. In MCF-7 cells the expression of cathepsin B was shown to depend on their activation with TGF-b1 while, for cathepsin X, a TGF-b1 independent mechanism of induction during EMT is indicated. PMID: 28495172
  4. these data suggest that apoptosis is induced by increasing lysosomal membrane permeability and leakage of CTSB into cytoplasm. PMID: 28478025
  5. These findings indicate that Cathepsin B (CTSB), may be an important prognostic biomarker for late stage colorectal cancer (CRC)and cases with lymph node metastases in the Middle Eastern population. Monitoring serum CTSB in CRC patients may predict and/or diagnose cases with lymph node metastases. PMID: 28440429
  6. Findings from this community-based cohort of young children show that surrogate markers for cardiovascular disease such as total fat mass, percent body fat, abdominal fat, body fat distribution, maximal oxygen uptake and pulse pressure were all associated with cystatin B. This was not found for cathepsin L or cathepsin D PMID: 29149174
  7. Interruption of either CCL2-CCR2 signaling or cathepsin B function significantly impaired perineural invasion (PNI). PMID: 28951461
  8. Data suggest that expression of CTSB in human glioblastoma cell line can be modulated by dietary factors; here, caffeine decreases tumor size and CTSB expression in mouse xenograft model of glioblastoma; note that caffeine, normally a dietary component, was administered via intraperitoneal injection in these studies. PMID: 27260469
  9. SNPs in NLRP3 and CTSB, associated with an increased NLRP3-inflammasome activation, are protective against the development of active pulmonary tuberculosis. PMID: 27101784
  10. cathepsin B activity assays identified secreted cathepsin B as responsible for apoA-I cleavage at Ser(228) Importantly PMID: 27630170
  11. ctsB enhanced neutrophil migration though a putative effect on pseudopod retraction rates. PMID: 28389621
  12. We have shown that keratolytic winter erythema in South African and Norwegian families is caused by two different tandem duplications in a non-coding genomic region upstream of CTSB. PMID: 28457472
  13. CtsB/L as major regulators of lysosomal function and demonstrate that CtsB/L may play an important role in intracellular cholesterol trafficking and in degradation of the key AD proteins PMID: 27902765
  14. in CTSB knockout (KO) mice, running did not enhance adult hippocampal neurogenesis and spatial memory function. Interestingly, in Rhesus monkeys and humans, treadmill exercise elevated CTSB in plasma. PMID: 27345423
  15. Report non-proteolytic cathepsin B functiona in tumor cell lines. PMID: 27526672
  16. cathepsin B (CTSB) inhibition or expression of a CTSB-resistant Dab2 mutant maintains Dab2 expression and shifts long-term TGF-beta-treated cells from autophagy to apoptosis PMID: 27398911
  17. cathepsin L and cathepsin B as the lysosomal cysteine proteases that activate the PEDV spike. PMID: 27729455
  18. Results showed an increase level of CTSL, and CTSB in dilated cardiomyopathy patients which correlates with reduced left ventricular ejection fraction. PMID: 28074340
  19. Gene expression level of genes of CTSB is significantly higher in AD patients when compared to normal controls. PMID: 26943237
  20. Serum CTSB and CTSD concentrations were found to have a diagnostic value in NPC. However, the CTSB and CTSD serum levels had no prognostic role for the outcome in nasopharyngeal carcinoma patients. PMID: 26995190
  21. CTSB might be involved in the development and progression of hepatocellular carcinoma. PMID: 26896959
  22. Cathepsin B knockdown in oral cancer cells reduces cell migration. PMID: 27031837
  23. the authors applied the technique of nanoelectrode arrays to try to detect and compare cathepsin B activities in normal and breast cancer cells. It was found that protease activity correlated positively with the degree of malignancy cancer cells. PMID: 25959927
  24. shedding of surface proteins by extracellular cathepsins impacts intracellular signaling as demonstrated for regulation of Ras GTPase activity. PMID: 26081835
  25. High cathepsin is associated with drug resistance in neuroblastoma. PMID: 25883214
  26. The expressions of cathepsin B strongly correlated with the Mini-Mental State Examination scores of the Alzheimer disease. patients PMID: 25502766
  27. Integrin alphavbeta3 is required for cathepsin B-induced hepatocellular carcinoma progression PMID: 25572981
  28. The serum level of cathepsin L decreased with age, while cathepsin B remained no significant difference between young and aged individuals PMID: 25991043
  29. Cathepsins B and L activity correlates with the efficiency of reovirus-mediated tumor cell killing. PMID: 25633482
  30. enhanced activity and expression of cathepsin B contributes to an increased remodelling of extracellular matrix that favours leiomyoma growth PMID: 25577554
  31. SCD5 impairs SPARC and cathepsin B secretion in human melanoma cells and intracellular pH acidification. PMID: 25802234
  32. data suggest that CTSB is a pathophysiologically relevant protease that activates ENaC in cystic fibrosis airways PMID: 25260629
  33. Cathepsin B activity is increased in blood and CSF from HIV-infected cocaine users. PMID: 25209871
  34. CB was highly upregulated in human ESCC and its precursor lesions. The elevated CB expression in ESCC allowed in vivo and in vitro detection of ESCC xenografts in nude mice. PMID: 24618814
  35. Inactivation of tristetraprolin in chronic hypoxia provokes the expression of cathepsin B PMID: 25452305
  36. A4383C and C76G SNP in Cathepsin B is respectively associated with the high risk and tumor size of hepatocarcinoma. PMID: 25106406
  37. Overexpression of uPAR and cathepsin B increases expression of cytosolic p-JNK. PMID: 24699410
  38. Study shows that CTSB overexpression in the cancer cells increases growth of invasive ductal carcinoma in the MMTV-PyMT mouse model of breast cancer. PMID: 24077280
  39. Cathepsin B (CB) substantially contributes to the invasive phenotype of FLS that leads to joint destruction in RA. PMID: 24749816
  40. Cathepsin D and B activities in the serum of patients with urothelial bladder cancer are directly proportional to disease severity and significantly higher compared with control group. PMID: 25095637
  41. Cathepsin B produces full-length amyloid-beta (Ab(1-40/42)) and pyroglutamate amyloid-beta (pyroGluAb(3-40/42) and is a key drug target for treating Alzheimer's disease by reducing these amyloid-beta species PMID: 24595198
  42. Cathepsin B is a key drug target for reducing the brain damage and improving neuromuscular dysfunction resulting from traumatic brain injury (TBI). PMID: 24083575
  43. Correlation between higher CTSB expression and lower survival rate in human lung squamous cell carcinoma. PMID: 24139065
  44. archazolid reduces the activity of prometastatic proteases like cathepsin B in vitro and in vivo. PMID: 24166050
  45. These observations point to a critical regulatory role for that endogenous CD activity in dopaminergic cells in alpha-synuclein homeostasis which cannot be compensated for by increased Cathepsin B. PMID: 24138030
  46. Data indicate that wild type and mutant amyloid precursor proteins (APP) expression enhances the induction in cathepsin B after administration of proteasome inhibitor MG132. PMID: 24215712
  47. Data suggest that CTSB (cathepsin B) and CTSL (cathepsin L) of the autophagic-lysosomal proteolytic system are involved as the main proteolytic system in skeletal muscle during cancer cachexia development in patients with esophageal cancer. PMID: 24108784
  48. Depletion of Cathepsin B in vitro inhibited cell proliferation. PMID: 23708264
  49. Increased concentrations of cathepsins B, D and G in the proliferative eutopic endometrium may play a role in the implantation of endometrial tissue outside the uterine cavity. PMID: 23466190
  50. High cathepsin expression is associated with dysplasia and colitis-associated cancer. PMID: 23591598
  51. our data demonstrate that chronic UVA inhibits both cathepsin B and L enzymatic activity and that dual inactivation of both enzymes is a causative factor underlying UVA-induced impairment of lysosomal function in dermal fibroblasts. PMID: 23603447
  52. Modulation of in vitro and in vivo glioblastoma cells growth characteristics by IGFBP-4 fragment CIBP-4 is associated with anti-cathepsin B activity. PMID: 23633927
  53. Reduced cathepsin B expression, effected by RNA interference, mimicked pharmacological inhibition of the enzyme and confirmed the contribution of cathepsin B to apoptotic events induced by DENV in HepG2 cells. PMID: 23867824
  54. Thus, cathepsin L and cathepsin B are not required for human papillomavirus type 16 infection but instead appear to restrict infection. PMID: 23677785
  55. Data indicate protein secretion pathways activated by monosodium urate (MSU) in macrophages, and reveal a novel role for cathepsin B and Src, Pyk2, PI3 kinases in the activation of unconventional protein secretion. PMID: 23292187
  56. Cysteine cathepsins B, K, and L constitutively secreted by osteoblasts are part of the fine-tuned regulation of CXCL12 in the bone marrow. PMID: 22066471
  57. Cathepsin B promotes proliferation and premalignant progression of breast epithelial cells. PMID: 23667900
  58. The total cathepsin B activity and content calculated per gram of DNA were higher in Wharton's jelly than in the umbilical cord vessels, and the latter parameter was the lowest in the umbilical cord arteries. PMID: 23189279
  59. Downregulation of uPAR and cathepsin B quenched TGF-beta1-driven invasion and survival of meningioma cells in a XIAP-dependent manner. PMID: 23222509
  60. Up-regulation of cysteine cathepsins B and L and TGF-beta production/signaling were required for the activation of fibroblasts and their promotion of the invasive growth of melanoma cells. PMID: 23063511
  61. Highlighted the importance of uPAR and cathepsin B in the regulation of malignant stem cell self-renewal through hedgehog components, Bmi1 and Sox2. PMID: 23222817
  62. Reduced cathepsins B and D cause impaired autophagic degradation that can be almost completely restored by overexpression of these two proteases in Sap C-deficient fibroblasts. PMID: 22949512
  63. 4-tert-octylphenol can adversely affect human health by promoting cancer proliferation and metastasis through the amplification of cathepsins B and D via the estrogen receptor-mediated signaling pathway PMID: 23219593
  64. Seminal plasma cathepsin B was approximately 70 times higher, while the cathepsin L values were approximately 500 times higher and the cathepsin S values approximately 40 times higher in seminal plasma than in a group of serum samples. PMID: 22571763
  65. enhancing CatB activity could lower Abeta, especially Abeta42, in AD patients with or without familial mutations. PMID: 23024364
  66. The activity of cathepsin B and L in blood significantly increased with age. PMID: 22695494
  67. Gene expression profiles of ASC or CatB deficient human DCs show marked overlap with downregulation of genes implicated in DC function. PMID: 22732093
  68. the genetic polymorphism of CTSB A8422G (rs8898) was associated with a high risk for the clinicopathological development of oral cancer PMID: 22851129
  69. Cathepsin B may cleave ENaC extracellularly after being secreted or intracellularly, while ENaC is present in the Golgi or in recycling endosomes. PMID: 22782900
  70. Acid ceramidase, through sphingosine 1-phosphate, promotes an invasive phenotype in prostate cancer by causing overexpression and secretion of cathepsin B through activation and nuclear expression of Ets1. PMID: 22322590
  71. Elevated cathepsin B level is associated with invasive glioblastoma. PMID: 22287159
  72. the role of cathepsin B in neuronal cell death induced by HIV-infected macrophages PMID: 22693552
  73. Memory cells are protected from lysosomal breakdown by inhibition of CTSB; CTSB is a physiological target of (Spi)2A in memory CD8-positive T cell survival. PMID: 22745374
  74. Cathepsin B concentrations correlate well with grading and invasivity of tumors. PMID: 22467319
  75. up-regulation of endothelial CTSB due to Fli1 deficiency may contribute to the development of SSc vasculopathy PMID: 22384200
  76. Activation of the Nipah virus fusion protein is mediated by cathepsin B within the endosome. PMID: 22278224
  77. signaling network activates the transcription of cathepsin B gene via myeloid zinc finger-1 transcription factor that binds to an ErbB2-responsive enhancer element in the first intron of cathepsin B PMID: 22464443
  78. CTSB and FDFT1 are excluded as candidates for keratolytic winter erythema PMID: 21945151
  79. an acute siRNA-mediated loss of TRPML1 specifically causes a leak of lysosomal protease cathepsin B (CatB) into the cytoplasm. CatB leak is associated with apoptosis, which can be prevented by CatB inhibition. PMID: 22262857
  80. Cathepsin B may contribute to both cervical cancer cell proliferation and invasion. PMID: 22127599
  81. our findings established a prometastatic role for cathepsin B in distant metastasis PMID: 22266111
  82. Recently discovered biological roles of cathepsins L and B indicate their significance in human health and disease. PMID: 21925292
  83. Cathepsin B and uPAR downregulation reduced tumor growth and increased p27 nuclear expression in vivo. PMID: 21840777
  84. Cathepsin B endopeptidase specificity is primarily guided by a strong selectivity for glycine. PMID: 21967108
  85. Cathepsins B and L activate Ebola but not Marburg virus glycoproteins for efficient entry into cell lines and macrophages independent of TMPRSS2 expression. PMID: 22222211
  86. Analysis of the data led to the elucidation of a new molecular mechanism by which SPARC promotes cathepsin B-mediated melanoma invasiveness using collagen I and alpha2beta1 integrins as mediators. PMID: 21850018
  87. AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D. PMID: 21948970
  88. Over-expression of cathepsin B promotes TNF--induced vascular smooth muscle cells apoptosis; gene silencing of cathepsin B inhibited this effect. PMID: 21600167
  89. Data show that mRNA levels of the two main lysosomal enzymes (cathepsin B and L) were not changed but, conversely, genes related to calpain and caspase had a higher expression in the muscles of the patients. PMID: 21880013
  90. High expression of cathepsin B is closely associated with tumor infiltration and lymphatic metastasis of cervical cancer. PMID: 20584670
  91. These data suggest that adenovirus 5 infection induces endosomal membrane rupture and release lysosomal cathepsin B that leads to mitochondrial membrane disruption and release of reactive oxygen species from the mitochondria. PMID: 21835790
  92. The cathepsin B deserves further evaluation as therapeutic targets to develop disease modifying drugs to treat Alzheimer's disease. PMID: 21585286
  93. High-risk HPV types may be associated with oral carcinoma, by cell-cycle control dysregulation, contributing to oral carcinogenesis and the overexpression of mdm2, p27 and cathepsin B. PMID: 19811804
  94. Serum cathepsin B showed significantly higher values 4 days after surgery in comparison with samples collected prior to surgery PMID: 21250859
  95. CTSL and CTSB are capable of directly processing both alpha- and beta-protryptases from human mast cells to their mature enzymatically active products. PMID: 21562164
  96. Processing of human protryptase in mast cells involves cathepsins L, B, and C. PMID: 21742978
  97. These results suggest that endocytosis, endosomal acidification, and cathepsin B activity are involved in the CD4-independent entry of HIV-1. PMID: 21541353
  98. Data show that SAA can induce the expression of pro-IL-1beta and activation of the NLRP3 inflammasome via P2X(7) receptor and a cathepsin B-sensitive pathway. PMID: 21508263
  99. These results suggest that cathepsin B and PAI-1 are important biomarkers for the stratification of glioblastoma patients with respect to survival. PMID: 19774387
  100. Plasma cathepsin B levels were higher in persons with Alzheimer disease compared to healthy controls whereas there was no difference between diagnostic groups in cathepsin B levels in CSF. PMID: 20930303

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Subcellular Location Lysosome, Melanosome, Secreted, extracellular space
Protein Families Peptidase C1 family
Database Links

HGNC: 2527

OMIM: 116810

KEGG: hsa:1508

STRING: 9606.ENSP00000342070

UniGene: Hs.520898

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