The process of expressing the recombinant human CTSB protein in the E.coli requires the recombinant DNA gene formed by the integration of encoding gene for the 82-333aa of the human CTSB protein and N-terminal GST tag sequence, the expression vector that the recombinant DNA gene inserts into, the E.coli that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal GST-tagged recombinant CTSB protein was obtained. This recombinant CTSB protein is characterized by high purity (>90%, SDS-PAGE). This CTSB protein ran along the gel to the band of approximately 55 kDa molecular weight.
CTSB is a gene providing instruction of making a protein named Cathepsin B (also abbreviated as APPS) in human and belong to peptidase C1 family. Generally, APPS is lowly expressed in normal tissues and mainly exists in lysosomes. When extracellular proteins such as plasma protein, hormones and phagocytosed bacteria enter the cell, they are hydrolyzed by proteolytic enzymes in the lysosome and digested inside the cell, thereby maintaining a precise balance between protein synthesis and degradation, and at the same time with other proteins in the lysosome The hydrolases together mediate the non-Caspase apoptotic pathway.
||Greater than 90% as determined by SDS-PAGE.
||Amyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CathepsinB; CPSB; CTSB; cysteine protease; OTTHUMP00000116009; OTTHUMP00000229510; OTTHUMP00000229511; OTTHUMP00000229512; OTTHUMP00000229514; OTTHUMP00000229515; OTTHUMP00000229516; Preprocathepsin B
||Homo sapiens (Human)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.