Recombinant Human Claudin-9 (CLDN9), partial-Detergent (Active)

This Recombinant Human Claudin-9 (CLDN9) is a premium, high-purity membrane protein fragment designed for functional and structural studies. Its key advantage lies in the specialized detergent-based purification process, which is essential for solubilizing and stabilizing this multi-pass transmembrane protein in its native, active conformation. Expressed in mammalian cells, the recombinant CLDN9 protein corresponds to amino acids 2-217 of human CLDN9 and is engineered with both an N-terminal 10xHis tag and a C-terminal Twin-Strep tag for highly efficient two-step purification.

The exceptional quality of this preparation is rigorously validated. SDS-PAGE analysis under reducing conditions confirms a purity greater than 95%, showing a clean, single band. Most importantly, its functional integrity is demonstrated through a quantitative binding ELISA: when immobilized, the protein exhibits specific, high-affinity interaction with an anti-CLDN6/9 recombinant antibody, yielding an EC₅₀ of 2.812-3.524 ng/mL. This confirms that the extracellular domains are correctly folded and accessible. With low endotoxin levels (<1.0 EU/µg), this ready-to-use reagent provides an indispensable tool for demanding applications such as ligand-binding assays, antibody characterization, and structural studies targeting the native state of the CLDN9 protein.