Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU). The EC50 is 2.812-3.524 ng/mL.

Target Names

CLDN9

Uniprot No.

O95484

Alternative Names

Claudin-9; CLDN9

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

2-217aa

Target Protein Sequence

ASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV

Mol. Weight

27.3 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

50 mM HEPES,0.15 M NaCl, 0.05% DDM,0.01% CHS,pH7.5

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Claudin-9 plays a critical role in tight junction assembly and barrier function, and its aberrant expression has been implicated in tumor progression and metastasis across multiple cancer types. This mammalian-expressed protein spanning residues 2–217 retains native post-translational modifications that support proper folding of the extracellular loops essential for homotypic and heterotypic claudin interactions. Functional validation demonstrates specific binding to an anti-CLDN6/9 recombinant antibody with an EC50 of 2.812–3.524 ng/mL in ELISA format, confirming that the extracellular epitopes remain accessible and correctly presented—a prerequisite for antibody development and validation studies targeting claudin family members. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein meets the quality thresholds commonly required for cell-based assays investigating tight junction dynamics, tumor cell migration and invasion, and the molecular mechanisms underlying claudin-mediated changes in epithelial barrier integrity.

Target Background

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Gene References into Functions

CLDN9 may be an important biomarker for invasive pituitary oncocytomas. PMID: 25281028
Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis. PMID: 19960275
Mouse studies suggest that claudin-9 deficiency may cause hearing loss not only in mice but also in humans. PMID: 19696885
Residues N38 and V45 in the first extracellular loop (EL1) of CLDN9 are necessary for HCV entry. PMID: 17804490
CLDN9, clustered with CLDN6 at human chromosome 16p13.3, is a four-transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C. PMID: 12736707

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Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Protein Families

Claudin family

Tissue Specificity

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

Database Links

HGNC: 2051

OMIM: 615799

KEGG: hsa:9080

STRING: 9606.ENSP00000398017

UniGene: Hs.296949

Code	CSB-MP005511HU1-D
Abbreviation	Recombinant Human CLDN9 protein, partial-Detergent (Active)
MSDS	MSDS Datasheet
Size	$725
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU). The EC₅₀ is 2.812-3.524 ng/mL. Biological Activity Assay
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Recombinant Human Claudin-9 (CLDN9), partial-Detergent (Active)

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