Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 μg/mL can bind Anti-IL2RA recombinant antibody (CSB-RA011649MA1HU) , the EC50 is 2.463-3.353 ng/mL.②Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 μg/mL can bind Human IL2(CSB-MP011629HU) , the EC50 is 1.693-2.039 ng/mL.

Target Names

IL2RA

Uniprot No.

P01589

Alternative Names

IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

22-213aa

Target Protein Sequence

ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC

Mol. Weight

23.6 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human interleukin-2 receptor subunit alpha (IL2RA) is generated in the mammalian cell expression system. The gene encoding the human IL2RA protein (22-213aa) is inserted into a plasmid vector, along with a C-terminal 10xHis-tag gene. This recombinant vector is transfected into mammalian cells. The transfected cells are cultured under controlled conditions that promote protein expression. The recombinant human IL2RA protein is harvested from the cell culture media. Its purity was measured to be greater than 95% using SDS-PAGE. The endotoxin content of this protein was determined to be less than 1.0 EU/ug using the LAL method. It reacted with both IL2RA recombinant antibody (CSB-RA011649MA1HU) and human IL2 (CSB-MP011629HU), demonstrating its bioactivity.

IL2RA, a specific component of the high-affinity IL2R, is primarily expressed on the surface of activated T cells, B cells, subsets of thymic cells, pre-B cells, and Treg cells. IL2RA exhibits high expression on the surface of various hematological tumor cells and is associated with tumor prognosis. Moreover, the elevated expression of IL2RA on activated circulating immune cells and Treg cells has been utilized in IL-2 immunotherapy for treating tumors and autoimmune diseases. Currently, the administration of anti-IL2RA radioimmunoconjugates and immunotoxins has been established as relatively clinically safe and effective in various hematological tumor indications. Additionally, novel antibody-drug conjugates (ADCs) targeting IL2RA are actively undergoing clinical trials. Developing mammalian IL2RA protein with high activity holds significant importance in facilitating research on IL2RA-related drugs.

Target Background

Function

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Gene References into Functions

Results are in line with the hypothesis that in the early phase of ALS, neuroprotective helper T cells infiltrate in the affected areas in the lumbar spinal cord. This was reflected in higher peripheral percentage of CD4(+) helper T cells and higher expression of FOXP3 and IL-2Ralpha. PMID: 29574662
a significant association was found between IL2RA SNP and susceptibility to alopecia areata in Iranian cohort PMID: 29979892
The gene polymorphisms at the loci of IL2RA rs2104286 and rs12722489 are closely associated with susceptibility to MS in the Chinese. PMID: 30352019
Higher frequency of IL2RA SNP could not be detected among multiple sclerosis patients. The difference of frequencies was statistically insignificant between groups, probably, due to low power of analysis and inadequate sample size. PMID: 29141792
Our meta-analysis suggests that the rs2104286 A allele is associated with increased MS risk in both Caucasians and Asians, whereas the rs12722489 C allele is associated with elevated MS risk in Caucasians but not in Asians. PMID: 29648897
sIL-2R could be a promising new marker for determining inflammatory disease activity in CRPS. PMID: 28634419
Relationship between soluble CD25 and gene expression in healthy individuals and patients with multiple sclerosis. PMID: 28511943
Increased serum level of soluble interleukin-2 receptor is associated with a worse response of metastatic clear cell renal cell carcinoma to interferon alpha and sequential VEGF-targeting therapy. PMID: 28545581
Data show that interleukin-2 receptor alpha, tumor necrosis factor receptor 1, serum STimulation-2 (IL1RL1 gene product), and regenerating islet-derived 3-alpha were significantly associated with non-relapse mortality. PMID: 28126963
High IL2RA expression is associated with CRLF2-rearranged acute lymphoblastic leukemia. PMID: 28866095
Inhibits CD25 translation through regulation of the LKB1-AMPK-mTOR pathway to suppress T cells. PMID: 28230853
Among patients with a low pretreatment sIL-2R level who exhibited a positive response to R-CHOP, the posttreatment sIL-2R level may help to identify those with a poor prognosis. PMID: 28413914
The findings not only confirm the predictive power of CD25 expression for Philadelphia chromosome translocation (Ph)+ but also demonstrate that CD25 expression is associated with RD (a biomarker correlated with prognosis) in Ph- patients. The latter finding is likely associated with underlying molecular abnormalities, including Ph-like genotype. PMID: 28430957
Through GWAS, we found that emotion dysregulation is associated at genome-wide level significance in a sex-specific manner, with a SNP in IL2RA in men. PMID: 27643478
it has been shown that CD25 serves as a negative growth regulator of Chronic myeloid leukemia leukemic stem cells. PMID: 28457753
Decidual stromal cells affect IL-2 production and IL-2R expression and signaling. PMID: 27651429
The single nucleotide variant rs12722489 determines differential ERalpha binding and enhancer properties of an IL2RA intronic region. PMID: 28234966
CDK6-mediated suppression of CD25 is required for initiation of T-ALL by activated Notch1 PMID: 26707936
CD4(+) CD25(+) GARP(+) Treg cells are defective in dilated cardiomyopathy patients and GARP seems to be a better molecular definition of the regulatory phenotype. PMID: 28207945
Studies indicate many potential uses of soluble interleukin-2 receptor measurement in the diagnosis and treatment of hemophagocytic syndromes. PMID: 28497365
Data suggest that differential methylation of the IL2RA promoter in T cells could be an important pathogenic mechanism in multiple sclerosis. PMID: 28077880
Sustained STAT5 transcription factor (STAT5) phosphorylation is necessary to induce long-term interleukin 2 receptor subunit alpha (CD25) expression in T lymphocytes. PMID: 27936140
Gastric adenocarcinoma patients present with increased PD-1(+) lymphocytes and CD4(+)CD25(+)FOXP3(+) regulatory T cells in the peripheral blood. PMID: 28031121
The serum concentration of soluble IL2 receptor are increased in patients with Kawasaki disease. (Review) PMID: 28081636
CD25 is an independent prognostic factor in elderly AML patients. Alternative therapies for CD25-positive elderly AML patients are needed. PMID: 28097942
The study demonstrated Increased level of CD25 in patients with active vitilligo. PMID: 27556155
CD45RA distinguishes CD4+CD25+CD127-/low TSDR demethylated regulatory T cell subpopulations with differential stability and susceptibility to tacrolimus-mediated immunosuppression. PMID: 28118317
Combination with anti-programmed cell death protein-1 (PD-1) antibodies promoted complete tumor rejection, indicating the relevance of CD25 antigen as a therapeutic target and promising substrate for future combination approaches in immune-oncology. PMID: 28410988
A compensatory mechanism of IL-7-mediated homeostatic proliferation can restore the inhibitory network of CD24+Foxp3+Treg cell after anti-CD25 induction therapy in islet allotransplantation. PMID: 27306531
(99) Tc-methylene diphosphonate may improve the activity of RA through upregulating the frequency of peripheral gammadelta T cells and CD4(+) CD25(+) Foxp3(+) Tregs as well as affecting the serum cytokine environment by increasing TGF-beta and decreasing TNF-alpha and IL-6. PMID: 24467668
IL-2Ra Interleukin-2 receptor antagonists (IL-2Ra) reduces the risk on new-onset diabetes after transplantation (NODAT) in liver transplant recipients. PMID: 26588180
The percentages of CD8(+)CD25(+)FoxP3(brigh) Tregs correlate with mean peak expiratory flow. PMID: 25921629
Efficiently downregulated the percentages of CD4+CD25+Foxp3+ regulator T (Treg) cells. PMID: 27431260
introduce the combined use of CD25 and properly strati fi ed CD135 values as alternatives to testing for the FLT3-ITD mutation PMID: 27087256
this study shows that PTPN22 genetic polymorphisms play role in predisposition of type 1 diabetes mellitus in Egyptian children PMID: 27288719
dissected the first intron of the IL2RA gene and selected several single nucleotide polymorphisms (SNPs) that may influence the regulation of the IL2RA gene in cell types relevant to autoimmune pathology PMID: 27876533
Its levels correlate to disease stage, assess response to therapy and are predictive of recurrence or better survival. We suggest, therefore, using sIL-2R as a reliable prognostic marker in HNC patients as a single marker, or in a combined panel of biomarkers. PMID: 27466555
CD25 had an adverse prognostic impact on these patients, and this poor prognosis may not be overcome, even with transplant. Patients with AML with residual CD25-positive blasts at the time of transplant may require additional therapy before or after transplant to improve survival. PMID: 26422713
IL2RA and TAGAP are novel vitamin D target genes. The vitamin D response is observed in samples from both the multiple sclerosis (MS) patients and controls, and is not dependent on the genotype of MS-associated SNPs in the respective genes. PMID: 26765264
Anti-CD25 recombinant immunotoxin LMB-2 had phase I activity limited by immunogenicity and rapid growth. PMID: 26350263
Enhanced pretreatment CD25 expression on CD4+ T cells was associated with decreased survival rate of acute myeloid leukemia patients. PMID: 26721345
A decrease of CD4(+) CD25(+) CD127(low) FoxP3(+) regulatory T cells with impaired suppressive function had been found in untreated ulcerative colitis patients. PMID: 26333292
Data show that an ultra-high level of serum serum soluble interleukin-2 receptor (sIL-2R) at diagnosis is a significant poor prognostic biomarker for angioimmunoblastic T-cell lymphoma (AITL). PMID: 25563559
The rs2104286 G allele in IL2RA is present at higher frequencies in neuromyelitis optica patients than in healthy controls within a Southern Han Chinese population. PMID: 24257225
Our results suggest that the CD34/CD25/CD123/CD99(+) LAIP is strictly associated with FLT3-ITD-positive cells. PMID: 25957287
Interleukin-2 Receptor alpha-Chain (CD25) Expression Predicts a Poor Prognosis in Acute Myeloid Leukemia PMID: 26375984
Data show that interleukin 2 receptor subunit alpha (IL2RA)-single nucleotide polymorphism rs2104286 and serum sIL2Ralpha-level associated with rheumatoid arthritis (RA)-persistence. PMID: 26350950
CD4+CD45RO+CD25-/lowCD127+: CD4+CD45RO+CD25hiCD127-/low ratio in peripheral blood indicates heart transplant recipients at risk for cardiac allograft vasculopathy. PMID: 25539460
analysis of sIL-2R levels in sarcoidosis patients with renal insufficiency PMID: 25745051
all five single nucleotide polymorphisms in the IL2RA gene are risk factors for type 1 diabetes risk. PMID: 26249556

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Involvement in disease

Diabetes mellitus, insulin-dependent, 10 (IDDM10); Immunodeficiency 41 with lymphoproliferation and autoimmunity (IMD41)

Subcellular Location

Membrane; Single-pass type I membrane protein.

Database Links

HGNC: 6008

OMIM: 147730

KEGG: hsa:3559

STRING: 9606.ENSP00000369293

UniGene: Hs.231367

Code	CSB-MP011649HU3
Abbreviation	Recombinant Human IL2RA protein, partial (Active)
MSDS	MSDS Datasheet
Size	$118
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2μg/mL can bind Anti-IL2RA recombinant antibody (CSB-RA011649MA1HU)，the EC₅₀ is 2.463-3.353 ng/mL. Biological Activity Assay Activity Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2μg/mL can bindHuman IL2(CSB-MP011629HU)，the EC₅₀ is 1.693-2.039 ng/mL. Biological Activity Assay
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Recombinant Human Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)

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