The recombinant CEACAM5 protein from Macaca fascicularis is a biologically active molecule produced in a mammalian expression system, encompassing the amino acids 1–685 of the native CEACAM5 to ensure native-like structure and function. It includes a C-terminal 10xHis tag for ease of purification and detection. This recombinant CEACAM5 protein is provided as a lyophilized powder and demonstrates exceptional purity—over 95%, as confirmed by both SDS-PAGE and SEC-HPLC analyses. Endotoxin content is rigorously controlled and remains below 1.0 EU/µg, as tested by the LAL method. Functional validation was conducted using ELISA, where the CEACAM5 protein, immobilized at 2 μg/mL, binds specifically to the anti-CEACAM5 recombinant antibody (CSB-RA005165MA4HU), yielding an EC50 between 3.572 and 4.044 ng/mL. These features make it highly reliable for use in immunological and biochemical assays involving CEACAM5.
The CEACAM5 protein plays a significant role in various biological processes, including cell adhesion, signaling, and immune responses in primates such as the rhesus macaque (Macaca mulatta). As a member of the CEACAM family, CEACAM5 is implicated in tumor progression and metastasis, particularly in epithelial tissues, where it can modulate cellular interactions and influence cell motility [1][2].
In the context of Macaca mulatta, CEACAM5 is involved in the pathophysiology of certain diseases, including cancers. The protein's expression can be upregulated in various carcinomas, and its interaction with other proteins can facilitate tumor cell adhesion and extravasation [3][4]. This is particularly relevant considering that the rhesus macaque model is frequently employed in biomedical research to study human diseases, including cancer, due to its close genetic and physiological similarities to humans [5][6].
Moreover, CEACAM5 has relevance concerning the immune response, as it may influence T-cell mediated immunity. The regulation of CEACAM5 can alter the immune landscape in tumors, affecting both tumor immunogenicity and the efficacy of therapeutic interventions [7][8]. The CEACAM5 protein's role as a potential biomarker for diagnosis and treatment response in cancers in macaque models can also provide insights into human health and disease management, highlighting its importance in translational medicine research [9][10].
Additionally, research utilizing bioinformatics and genomic analyses has contributed to our understanding of CEACAM5's structure and function. Recently, studies have applied high-throughput sequencing methods to profile CEACAM5 and related gene expressions across various tissues [11][12][4], suggesting a functional diversity that reflects evolutionary adaptations among primates, including Macaca mulatta.
References:
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