ATF4 Antibody

Code CSB-PA002272LA11HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate
    All lanes: ATF4 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39 kDa
    Observed band size: 50 kDa

  • IHC image of CSB-PA002272LA11HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ATF4 Polyclonal antibody
Uniprot No. P18848
Target Names ATF4
Alternative Names Activating transcription factor 4 antibody; ATF 4 antibody; ATF4 antibody; ATF4 protein antibody; ATF4_HUMAN antibody; cAMP-dependent transcription factor ATF-4 antibody; cAMP-responsive element-binding protein 2 antibody; CREB 2 antibody; CREB-2 antibody; CREB2 antibody; Cyclic AMP dependent transcription factor ATF 4 antibody; Cyclic AMP response element binding protein 2 antibody; Cyclic AMP-dependent transcription factor ATF-4 antibody; Cyclic AMP-responsive element-binding protein 2 antibody; DNA binding protein TAXREB67 antibody; DNA-binding protein TAXREB67 antibody; Tax Responsive Enhancer Element B67 antibody; Tax-responsive enhancer element-binding protein 67 antibody; TaxREB67 antibody; TXREB antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Cyclic AMP-dependent transcription factor ATF-4 protein (20-200aa)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:100-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional activator. Binds the cAMP response element (CRE) (consensus
Gene References into Functions
  1. phosphorylated PERK and ATF4 would be upregulated in Orexin neurons in Sudden Infant Death Syndrome (SIDS) compared to non-SIDS. PMID: 27796753
  2. Our data suggests a novel interaction between Nrf2 and ATF4 under oxidative and endoplasmic reticulum stress, thus drives specific enzymatic and non-enzymatic reactions of antioxidant mechanisms maintaining redox homeostasis. PMID: 29421327
  3. PSAT1, which is overexpressed in ER-negative breast cancers, is activated by ATF4 and promotes cell cycle progression via regulation of the GSK3beta/beta-catenin/cyclin D1 pathway. PMID: 29216929
  4. POSTN may function as a protective factor for osteoblasts during this process by inhibiting the eIF2alphaATF4 pathway. PMID: 29207036
  5. p62 directly targets nuclear transcription factors to control metabolic reprogramming in the microenvironment and repress tumorigenesis, and identifies ATF4 as a synthetic vulnerability in p62-deficient tumor stroma. PMID: 28988820
  6. results suggest a conditional regulation of KRT16 gene by ATF4 that may be inhibited in normal cells, but engaged during cancer progression. Potential roles of KRT16, FAM129A and HKDC1 genes upregulation in adaptive stress responses and pathologies are discussed PMID: 29420561
  7. Results provide evidence that the availability of glucose controls ATF4-mediated MITF suppression to drive melanoma cell proliferation. PMID: 28380427
  8. Decreased ATF4 expression as a mechanism of acquired resistance to long-term amino acid limitation in cancer cells PMID: 28460466
  9. These results suggest that p21 induction plays a vital role in the cellular response to ER stress and indicate that p21 is a prosurvival effector of ATF4. PMID: 28975618
  10. GRP78 inhibition enhances ATF4-induced cell death by the deubiquitination and stabilization of CHOP in human osteosarcoma cells. PMID: 28947141
  11. Expression of either dominant-negative or constitutively active mutants of Nrf2, ATF4, or c-Jun confirmed that distinct transcription units are regulated by these transcription factors. PMID: 27278863
  12. ATF4 contributes to tumor growth of endometrial cancer (EC) by promoting CCL2 and subsequent recruitment of macrophage, and ATF4/CCL2 axis might be a potential therapeutic target for EC. PMID: 28843961
  13. ATF4 expression fosters the malignancy of primary brain tumors and increases proliferation and tumor angiogenesis; experiments revealed that ATF4-dependent tumor promoting effects are mediated by transcriptional targeting the glutamate antiporter xCT PMID: 28553953
  14. The PERK-eIF2alpha-ATF4-CHOP signaling pathway has a critical role in tumor progression during endoplasmic reticulum stress. (Review) PMID: 27211800
  15. ATF4 pathway is activated in vivo upon mitochondrial stress. PMID: 28566324
  16. a shortage of tryptophan caused by expression of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) resulted in ATF4-dependent upregulation of several amino acid transporters. PMID: 27651314
  17. SLC30A10 has a protective role in 1-methyl-4-phenylpyridinium-induced toxicity via PERK-ATF4 pathway. PMID: 28688763
  18. There was decreased or loss of ATF4 in 52% of medullary thyroid cancer (MTC) tumors (n = 39) compared with normal thyroid follicle cells. A negative correlation was observed between RET and ATF4 protein levels in MTC tumors. PMID: 27935748
  19. hypoxia-induced ATF4 expression may promote progression of proliferating infantile hemangioma through macrophage colony-stimulating factor induced M2-polarized macrophages infiltration. PMID: 28438094
  20. overexpression of eIF5 and 5MP induces translation of ATF4. PMID: 27325740
  21. ATF4 may exert various physiological roles in lipid metabolism depending on the nutrient composition. In addition, these results suggested that ATF4 has a role in regulating lipogenesis and in the development of NAFLD; thus ATF4 may be considered a therapeutic target for NAFLD. PMID: 27357269
  22. PERK-eIF2alpha-ATF4 signaling pathway mediated by endoplasmic reticulum stress involved in osteoblast differentiation of periodontal ligament cells under cyclic mechanical force. PMID: 27079961
  23. The localization of ATF4 in the granular component of nucleoli together with its association with nascent RNA transcripts in cells undergoing proteotoxic cell stress could suggest a new function for ATF4 in cell stress management. PMID: 27567537
  24. The results showed that the FGF21 promoter contains three response elements for ATF4, suggesting that FGF21 is a sensitive target of ATF4. PMID: 27010621
  25. ADM-2 is a stress-inducible gene controlled by ATF-4. PMID: 27328454
  26. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
  27. High ATF4 expression is associated with osteosarcoma progression. PMID: 26797758
  28. miR-214 directly targeted ATF4, a crucial transcriptional factor involved in anti-stress responses, down regulation of miR-214 releases the repression of ATF4 translation and leads to increased ATF4 protein content. PMID: 26791102
  29. The activation of ATF4 in response to ONC201 required the kinases HRI and PKR, which phosphorylate and activate the translation initiation factor eIF2alpha. PMID: 26884600
  30. TBL2 participates in ATF4 translation through its association with the mRNA. PMID: 26239904
  31. Inhibition or overexpression of ATF4 confirms the role of ATF4 in SESN2 gene up-regulation induced by mitochondrial dysfunction. PMID: 26771712
  32. ATF4 and ATF6beta act synergistically in the negative regulation of placental growth factor mRNA expression PMID: 26648175
  33. Authors observed that a slow rate of ATF4-translation and late re-initiation of general translation coincided with cells which were resistant to ER stress-induced cell death. PMID: 25633195
  34. a reduction of cell death was associated with decreased levels of ATF4 in a rhabdomyosarcoma cell line PMID: 26172539
  35. Combined administration inhibited the cells most potently and time-dependently, decreased the expression of HO-1, and significantly increased the expression of ATF4, CHOP, and Ire-1 proteins expression levels PMID: 26125799
  36. Global profiling in human mesenchymal stem cells and a novel cell-free assay reveals that ATF4 requires C/EBPbeta for genomic binding at a motif distinct from that bound by the C/EBPbeta homodimer. PMID: 26111340
  37. This study outlines the mechanism of NIR laser phototoxicity and the utility of monitoring surface temperature and ATF4 expression as potential biomarkers to develop safe and effective clinical applications. PMID: 26030745
  38. Up-regulation of ATF4 is associated with Pancreatic Neuroendocrine Tumors. PMID: 26504039
  39. The ATF4/p75NTR/IL-8 signal pathway may have an important role in EndoMT induced by SFO. PMID: 24905361
  40. ATF4 is a potential biomarker for esophageal squamous cell carcinoma (ESCC) prognosis and its dysregulation may play a key role in the regulation of invasion and metastasis in ESCC. PMID: 25078779
  41. Upon loss of attachment in tumor cells, ATF4 activated a program of cytoprotective autophagy and antioxidant responses, including induced expression of heme oxygenase 1 (HO-1). Increased levels of HO-1 ameliorated oxidative stress and cell death. PMID: 26011642
  42. Treatment with a skin sensitizer rapidly induces the phosphorylation of eIF2a and a concomitant increase of ATF4 protein levels in dendritic cells. PMID: 25236743
  43. The results demonstrate that the endoplasmic reticulum stress-regulated ATF4/p16 pathway is involved in the premature senescence of renal tubular epithelial cells during diabetic nephropathy progression. PMID: 25567807
  44. RET as a novel dual kinase with nuclear localization and provide mechanisms by which RET represses the proapoptotic genes PMID: 25795775
  45. A sustained deficiency of mitochondrial respiratory complex III induces an apoptotic cell death through the p53-mediated inhibition of pro-survival activities of the ATF4. PMID: 25375376
  46. ATF4 signaling pathway is essential for mediating the effect of ER stress on beta-klotho expression. PMID: 25727012
  47. B-cell lymphoma/leukemia 10 promotes oral cancer progression through STAT1/ATF4/S100P signaling pathway. PMID: 24681956
  48. ATF4-mediated repression of apelin contributes substantially to the pro-apoptotic effects of p38. PMID: 25052841
  49. Bone diseases of diabetes mellitus type 2 showing definite changes of ATP4 gene expression. PMID: 24715035
  50. the PERK/ATF4/LAMP3-arm of the UPR is an additional pathway mediating hypoxia-induced breast cancer cell migration PMID: 23294542

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Subcellular Location Cytoplasm, Cell membrane, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Protein Families BZIP family
Database Links

HGNC: 786

OMIM: 604064

KEGG: hsa:468

STRING: 9606.ENSP00000336790

UniGene: Hs.496487

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