ATG4A Antibody

Datasheet
Code CSB-PA547032
Size US$299
Uniprot No. Q8WYN0
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA547032(ATG4A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA547032(ATG4A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 231 cells, human liver cancer tissue, Primary antibody: CSB-PA547032(ATG4A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Synthetic peptide of Human ATG4A
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA,WB,IHC;ELISA:1:2000-1:5000,WB:1:200-1:1000,IHC:1:25-1:100
Relevance Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias autophagy related 4A, cysteine peptidase
Immunogen Species Homo sapiens (Human)
Target Names ATG4A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Subcellular Location Cytoplasm
Protein Families Peptidase C54 family
Tissue Specificity Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Database Links

HGNC: 16489

OMIM: 300663

KEGG: hsa:115201

STRING: 9606.ENSP00000361306

UniGene: Hs.8763

Pathway Autophagy

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