ATG4A Antibody

Datasheet

Code	CSB-PA547032
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA547032(ATG4A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA547032(ATG4A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 231 cells, human liver cancer tissue, Primary antibody: CSB-PA547032(ATG4A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q8WYN0

Target Names

ATG4A

Alternative Names

AI627006 antibody; APG4 autophagy 4 homolog A antibody; Apg4a antibody; ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody; ATG4 autophagy related 4 homolog A antibody; ATG4A antibody; ATG4A_HUMAN antibody; Atg4al antibody; AUT like 2 cysteine endopeptidase antibody; AUT-like 2 cysteine endopeptidase antibody; Autl2 antibody; Autophagin 2 antibody; Autophagin-2 antibody; Autophagy related 4A cysteine peptidase antibody; Autophagy related cysteine endopeptidase 2 antibody; Autophagy related protein 4 homolog A antibody; Autophagy-related cysteine endopeptidase 2 antibody; Autophagy-related protein 4 homolog A antibody; AV169859 antibody; Cysteine protease ATG4A antibody; hAPG4A antibody; MGC107179 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide of Human ATG4A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:200-1:1000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene References into Functions

An intron SNP rs807185 in ATG4A might be a protective factor for lung cancer. PMID: 26061994
ATG4A promotes the metastasis of gastric cancer cells via the Notch signaling pathway, which is an autophagy-independent mechanism PMID: 27276686
The structure and regulatory machinery of Atg4a [review] PMID: 28253956
We conclude that miR-24-3p regulates autophagy by targeting ATG4A PMID: 25426560
The autophagy regulator ATG4A was found to be essential for the maintenance of a sub-population with cancer stem cell properties and to regulate breast cancer cell tumourigenicity. PMID: 24229464
The demonstration that expression of ATG4A in cells increased stem properties provided an indication of its biological function. Hypomethylation of ATG4A and HIST1H2BN in ovarian tumor-initiating cells predicts poor prognosis for ovarian cancer patients. PMID: 24256813
no association between SNP rs807185 and the presence of granulomas in Crohn's disease PMID: 21122541
statistical analysis of APG4A gene confirmed the characterisation of each protein-coding gene present in human genome Xq22.1 PMID: 21297227
Atg4B possessed the broadest spectrum against all substrates, followed by Atg4A for ATG8 substrates PMID: 21177865
HsApg4A cleaves the COOH terminus of GATE-16 PMID: 12473658
The cysteine protease HsAtg4 is identified as a direct target for oxidation by hydrogen peroxide, and a cysteine residue located near the HsAtg4 catalytic site as critical for this regulation. PMID: 17347651

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Subcellular Location

Cytoplasm.

Protein Families

Peptidase C54 family

Database Links

HGNC: 16489

OMIM: 300663

KEGG: hsa:115201

STRING: 9606.ENSP00000361306

UniGene: Hs.8763

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Autophagy Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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