Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CASP10 Polyclonal antibody

Uniprot No.

Q92851

Target Names

CASP10

Alternative Names

ALPS2 antibody; Apoptotic protease Mch-4 antibody; CASP 10 antibody; CASP-10 antibody; CASP10 antibody; CASPA_HUMAN antibody; Caspase 10 apoptosis related cysteine peptidase antibody; Caspase-10 antibody; Caspase-10 subunit p12 antibody; FADD like ICE2 antibody; Fas associated death domain protein antibody; FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody; FLICE 2 antibody; FLICE2 antibody; ICE like apoptotic protease 4 antibody; ICE-like apoptotic protease 4 antibody; Interleukin 1B converting enzyme 2 antibody; MCH 4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from Human Caspase 10.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB,IHC,IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:3000
IHC	1:50-1:100
IF	1:100-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.; Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.; Isoform C is proteolytically inactive.

Gene References into Functions

Caspase-10 is a negative regulator of cell death and a facilitator of gene induction separates the function of cFLIP and caspase-10 in the death-inducing signaling complex. PMID: 28445729
This study reveals an essential role of SUMOylated FADD in Drp1- and caspase-10-dependent necrosis. PMID: 27799292
Three cases of autoimmune lymphoproliferative syndrome due to somatic FAS mutation (ALPS-sFAS) combined with a germline CASP10 variation have been described. PMID: 26323380
Results showed miR-221/222 promote cell proliferation and repress apoptosis, through suppressing caspase-10, in prostate cancer cells. PMID: 26164758
Single nucleotide polymorphisms in CASP10 gene is associated with gastric cancer. PMID: 23921907
While myeloma cells require a basal level of autophagy for survival, caspase-10 tempers this response to avoid cell death. PMID: 23541952
This meta-analysis suggests that the rs13006529 T carrier in the CASP-10 gene might be a risk factor for cancer susceptibility, especially for breast cancer. PMID: 23212337
The protein factor-arrest 11 (Far11) is essential for the toxicity of human caspase-10 in yeast and participates in the regulation of autophagy and the DNA damage signaling. PMID: 22782902
induces apoptosis in avian virus H5N1-infected human monocyte-derived macrophages PMID: 21911414
Caspase-10 expression, measured by quantitative real-time RT-PCR, is a possible prognostic factor in patients with stage II colorectal cancer. PMID: 21559821
Caspase-10 isoforms play distinct and opposing roles in the initiation of death receptor-mediated tumour cell apoptosis. PMID: 21368896
The CASP10 V410I polymorphism was not associated with breast or ovarian cancer risk for BRCA1 or BRCA2 mutation carriers. PMID: 20978178
Data show that caspase-10 is the most active caspase in apoptotic erythroid progenitors induced by 11 kDa and NS1 as well as during B19V infection. PMID: 19861680
Somatic mutation of CASP10 is rare in colon, breast, lung, and hepatocellular carcinomas. PMID: 20025484
CASP10 mutation might contribute to the pathogenesis of factions of T-acute lymphoblastic leukaemia and multiple myeloma. PMID: 19900088
inactivating mutations in non-hodgkin lymphoma. PMID: 12010812
Caspase-10 is recruited to & activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8. Caspase-10 is cleaved during CD95-induced apoptosis of activated T cells. PMID: 12198154
histone H3 phosphoacetylation at the CASPASE-10 gene may play an important role in the induction of apoptosis in acute promyelocytic leukemia cells in response to arsenic trioxide PMID: 12388546
over-expression of GRB2 and FLICE2 in RA synovium is caused by TNF-alpha inducibility differentially regulated in RA synoviocytes and provide potential pathogenic roles of these genes in the hyperplasia of the RA synovium. PMID: 14687710
CASP10 levels are increased following DNA damage in a p53-dependent manner. PMID: 14688482
Re molecular requirements for neutrophil apoptosis: TNF-alpha-mediated apoptosis depends on the activation of caspase-8, spontaneous apoptosis requires the activation of caspase-10/b. PMID: 14761933
taxol induces FADD-dependent apoptosis primarily through activation of caspase-10 but independently of death receptors PMID: 15452117
a model in which caspase-10 is a crucial component during TRAIL-mediated apoptosis PMID: 15767684
Role of variants of caspase-10 in Autoimmune Lymphoproliferative Syndrome. PMID: 16446975
Altogether, these data indicate an active role for caspase-10 in cytotoxic drug-induced tumor cell death, downstream of the mitochondria. PMID: 16767158
These results suggest that caspase-10, DR-3 and IGFBP-3 are involved in apoptosis in the preeclamptic placenta. PMID: 17085968
A novel prodomain-only isoform of caspase-10 may preferentially play a regulatory role in NF-kappa B pathways. PMID: 17822854
Fas expression was reduced and caspase-10 activity was decreased in both patients PMID: 17999750
Caspase-10 expression was down-regulated in both rectal adenomas and cancers PMID: 18716417
Single nucleotide polymorphism data suggest a role for CASP10 as a potential modifier of the asthma phenotype, specifically with measures of airway obstruction and bronchial hyperresponsiveness. PMID: 18823309
the effect of the ectopic expression of the human initiator caspases-10 in Saccharomyces cerevisiae PMID: 19166881
Results show that heat shock protein 90 beta is cleaved by activated caspase-10 under UVB irradiation. PMID: 19380486

Hide All

Involvement in disease

Autoimmune lymphoproliferative syndrome 2A (ALPS2A); Familial non-Hodgkin lymphoma (NHL); Gastric cancer (GASC)

Protein Families

Peptidase C14A family

Tissue Specificity

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Database Links

HGNC: 1500

OMIM: 601762

KEGG: hsa:843

STRING: 9606.ENSP00000286186

UniGene: Hs.5353

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address