CD27 Antibody

Datasheet
Code CSB-PA08235A0Rb
See More Details Free Antibody trial simple
Size US$299
Uniprot No. P26842
Image
  • Western Blot
    Positive WB detected in: Rat spleen tissue, Rat kidney tissue, Rat liver tissue
    All lanes: CD27 antibody at 3.2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • IHC image of CSB-PA08235A0Rb diluted at 1:800 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA08235A0Rb diluted at 1:800 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA08235A0Rb at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human CD27 antigen protein (20-191AA)
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Relevance Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias CD27 antigen (CD27L receptor) (T-cell activation antigen CD27) (T14) (Tumor necrosis factor receptor superfamily member 7) (CD antigen CD27), CD27, TNFRSF7
Immunogen Species Human
Research Area Immunology
Target Names CD27
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Involvement in disease Lymphoproliferative syndrome 2 (LPFS2)
Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Found in most T-lymphocytes.
Database Links

HGNC: 11922

OMIM: 186711

KEGG: hsa:939

STRING: 9606.ENSP00000266557

UniGene: Hs.355307

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