CHRM5 Antibody

Code CSB-PA005385LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: Mouse liver tissue, Mouse kidney tissue, Mouse brain tissue
    All lanes: CHRM5 antibody at 3.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA005385LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA005385LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CHRM5 Polyclonal antibody
Uniprot No.
Target Names
CHRM5
Alternative Names
CHRM5; Muscarinic acetylcholine receptor M5
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Muscarinic acetylcholine receptor M5 protein (220-427AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CHRM5 Antibody (Product code: CSB-PA005385LA01HU) is Non-conjugated. For CHRM5 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA005385LB01HU CHRM5 Antibody, HRP conjugated ELISA
FITC CSB-PA005385LC01HU CHRM5 Antibody, FITC conjugated
Biotin CSB-PA005385LD01HU CHRM5 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Gene References into Functions
  1. Immunological stimulation leads to M5 mAChR gene expression in lymphocytes. PMID: 12675126
  2. direct evidence suggesting that the M5 muscarinic receptor gene combined with the alpha7-nicotinic receptor gene may be linked to schizophrenia PMID: 15292665
  3. mAChR subtypes (m1 to m5)presents in human scleral fibroblasts at both mRNA and protein levels. PMID: 16877267
  4. the first study to show an association between CHRM5 and substance use in humans PMID: 17608938
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM5 sub-subfamily
Database Links

HGNC: 1954

OMIM: 118496

KEGG: hsa:1133

STRING: 9606.ENSP00000372750

UniGene: Hs.584747

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