IGF1R (Ab-1280) Antibody

Datasheet
Code CSB-PA443662
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P08069
Image
  • Western blot analysis of extracts from 293,3T3 and Hela cells using IGF-1R(Ab-1280) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R(Ab-1280) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around aa. 1278~1282 (S-F-Y-Y-S ) derived from Human IGF-1R .
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total IGF-1R protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200
Relevance This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.

Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093
Baserga, R. et al. (2000) Oncogene 19, 5574-5581
Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
O'Connor R, et al. Mol Cell Biol 1997 Jan; 17(1): 427-35
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias Insulin-like growth factor I receptor
Immunogen Species Homo sapiens (Human)
Target Names IGF1R
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways
Involvement in disease Insulin-like growth factor 1 resistance (IGF1RES)
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Tissue Specificity Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the
Database Links

HGNC: 5465

OMIM: 147370

KEGG: hsa:3480

STRING: 9606.ENSP00000268035

UniGene: Hs.643120

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Adherens junction
Autophagy
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
AMPK signaling
FoxO signaling pathway
Rap1 signaling pathway
Endocytosis

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