Your Good Partner in Biology Research
Tel : 301-363-4651
Your Good Partner in Biology Research
| Code | CSB-PA839346HD01HU |
| Size | US$299 |
| Uniprot No. | Q96J02 |
| Protocols | ELISA Protocol |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA) |
| Raised in | Rabbit |
| Species Reactivity | Human |
| Tested Applications | ELISA |
| Relevance | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation (By similarity). Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). |
| Form | Liquid |
| Conjugate | Biotin |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method | >95%, Protein G purified |
| Isotype | IgG |
| Clonality | Polyclonal |
| Alias | E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1), ITCH |
| Immunogen Species | Homo sapiens (Human) |
| Research Area | Cell Biology |
| Target Names | ITCH |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Function | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates |
| Involvement in disease | Autoimmune disease, multisystem, with facial dysmorphism (ADMFD) |
| Subcellular Location | Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, Nucleus, Early endosome membrane, Peripheral membrane protein, Cytoplasmic side, Endosome membrane, Peripheral membrane protein, Cytoplasmic side |
| Tissue Specificity | Widely expressed. |
| Database Links |
HGNC: 13890 OMIM: 606409 KEGG: hsa:83737 STRING: 9606.ENSP00000363998 UniGene: Hs.632272 |
| Pathway |
TNF signaling pathway
Ubiquitin mediated proteolysis Endocytosis |
ITCH Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA180347 | Phospho-ITCH (Tyr420) Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA168801 | ITCH (Ab-420) Antibody | Human,Mouse | ELISA,WB |
| CSB-PA060277 | Phospho-ITCH (Y420) Antibody | Human,Mouse | IHC,ELISA |
| CSB-PA060278 | ITCH Antibody | Human,Mouse | WB,ELISA |
| CSB-PA839346HA01HU | ITCH Antibody | Human | ELISA, WB, IF |
| CSB-PA839346HB01HU | ITCH Antibody, HRP conjugated | Human | ELISA |
| CSB-PA839346HC01HU | ITCH Antibody, FITC conjugated | Human | ELISA |
ITCH Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP839346HU CSB-EP839346HU CSB-BP839346HU CSB-MP839346HU |
Recombinant Human E3 ubiquitin-protein ligase Itchy homolog(ITCH) ,partial | Yeast E.coli Baculovirus Mammalian cell |
2-hydroxyisobutyryl-HIST1H4A (K5) Antibody
Applications: ELISA, WB, ICC, IF
Species Reactivity: Human
