MCU Antibody

Code CSB-PA847694LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: MCU antibody at 3.4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40, 37, 36 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA847694LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA847694LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MCU Polyclonal antibody
Uniprot No.
Target Names
MCU
Alternative Names
MCU; C10orf42; CCDC109A; Calcium uniporter protein, mitochondrial; HsMCU; Coiled-coil domain-containing protein 109A
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Calcium uniporter protein, mitochondrial protein (155-233AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MCU Antibody (Product code: CSB-PA847694LA01HU) is Non-conjugated. For MCU Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA847694LB01HU MCU Antibody, HRP conjugated ELISA
FITC CSB-PA847694LC01HU MCU Antibody, FITC conjugated
Biotin CSB-PA847694LD01HU MCU Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
Gene References into Functions
  1. MICU1 imparts the mitochondrial uniporter with the ability to discriminate between Ca(2+) and Mn(2+). PMID: 30082385
  2. Mitochondrial Ca(2+) uptake is controlled by protein arginine methyl transferase 1 that asymmetrically methylates MICU1, resulting in decreased Ca(2+) sensitivity. UCP2/3 normalize Ca(2+) sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca(2+) uptake activity. PMID: 27642082
  3. VDAC1 allows Ca(2+) access to the MCU, facilitating transport of Ca(2+) to the matrix, and also from the IMS to the cytosol. Intra-mitochondrial Ca(2+) controls energy production and metabolism by modulating critical enzymes in the tricarboxylic acid (TCA) cycle and fatty acid oxidation. PMID: 29594867
  4. MICU2 restricts spatial crosstalk between InsP3R and MCU channels by regulating threshold and gain of MICU1-mediated inhibition and activation of MCU. PMID: 29241542
  5. The results highlight the dynamic nature of uniporter subunit assembly, which must be tightly regulated to ensure proper mitochondrial responses to intracellular Ca(2+) signals. PMID: 28396416
  6. MCU expression returned to physiological levels in visceral adipose tissue of patients after weight loss by bariatric surgery. Altered mitochondrial calcium flux in fat cells may play a role in obesity and diabetes and may be associated with the differential metabolic profiles of visceral and subcutaneous adipose tissue. PMID: 28790027
  7. Mitochondrial calcium uniporter plays an important role in hyperglycaemia-induced endothelial cell dysfunction. PMID: 28777009
  8. The studies findings in aging human skeletal muscle confirm the data obtained in mice and propose mitochondrial calcium uniporter and mitochondria-related proteins as potential pharmacological targets to counteract age-related muscle loss. PMID: 28039397
  9. decreased MCU expression in hypertensive with mutation cells contributed to dysregulated Ca(2+) uptake into the mitochondria PMID: 27471128
  10. The molecular structure and regulation of the MCU complex in addition to its pathophysiological role are discussed with particular attention to striated muscle tissues. Review. PMID: 26968367
  11. High MCU expression is associated with metastasis in hepatocellular carcinoma. PMID: 28650465
  12. Here, the authors determine the transmembrane orientation of EMRE, and show that its known MCU-activating function is mediated by the interaction of transmembrane helices from both proteins. PMID: 27099988
  13. Regulation of mitochondrial Ca(2+) suggests that MCU may play a pivotal role in the development of fibrosis and could potentially be a therapeutic target for pulmonary fibrosis. PMID: 28351840
  14. MCU downregulation hampered cell motility and invasiveness and reduced tumor growth, lymph node infiltration, and lung metastasis in triple-negative breast cancer xenografts. In MCU-silenced cells, production of mitochondrial reactive oxygen species (mROS) is blunted and expression of the hypoxia-inducible factor-1alpha (HIF-1alpha) is reduced, suggesting a signaling role for mROS and HIF-1alpha, downstream of mitochon... PMID: 27138568
  15. Reveal a distinct functional role for Cys-97 in mitochondrial reactive oxygen species sensing and regulation of MCU activity. PMID: 28262504
  16. Study indicates that inhibition of mitochondrial calcium uniporter can inhibit excessive mitophagy and protect the neurocytes from ischemia/reperfusion injury. PMID: 27288019
  17. Our experiments provide novel details about how MCU/EMRE is regulated by MICU1 and an original approach to investigate MCU/EMRE activation in intact cells. PMID: 26489515
  18. results suggest that N terminal domain of MCU is essential for the modulation of MCU function, although it does not affect the uniplex formation PMID: 26341627
  19. MCU-VDAC1 complex regulates mitochondrial Ca(2+) uptake and oxidative stress-induced apoptosis PMID: 25753332
  20. Loss of heterozygosity of MCU gene on chromosome 10q is associated with pancreatic cancer. PMID: 25824785
  21. Studies indicate the existence of an inner mitochondrial protein termed the mitochondrial calcium uniporter for calcium transport. PMID: 25999421
  22. analyses establish that MCU encodes the pore-forming subunit of the uniporter channel PMID: 23755363
  23. MCU plays a critical role in breast cancer cell migration by regulating store-operated Ca(2) entry. PMID: 25640838
  24. We review not only the biochemical identities and structures of the proteins required for mitochondrial Ca2+ uptake but also their implications in different physiopathological contexts. [review] PMID: 24366263
  25. ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake PMID: 24815697
  26. study demonstrates that MCU overexpression is a feature of some breast cancers and that MCU overexpression may offer a survival advantage against some cell death pathways PMID: 23602897
  27. The mitochondrial calcium uniporter (MCU): molecular identity and physiological roles. PMID: 23400777
  28. the crucial role of MICU1 and MCU in mitochondrial Ca(2+) uptake in pancreatic beta-cells and their involvement in the positive feedback required for sustained insulin secretion. PMID: 22904319
  29. Functional characterization of the MCU protein; shows that it is necessary for mitochondrial calcium uptake. PMID: 21685888
  30. MCU is an oligomeric protein residing in the mitochondrial inner membrane that interacts with MICU1 and is necessary for mitochondrial calcium uniport. PMID: 21685886
  31. CCDC109A was identified in this large-scale proteomics analysis as a mitochondrial protein broadly expressed in many mouse tissues. The human protein was also confirmed to localize to mitochondria. PMID: 18614015

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Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Protein Families
MCU (TC 1.A.77) family
Database Links

HGNC: 23526

OMIM: 614197

KEGG: hsa:90550

STRING: 9606.ENSP00000362144

UniGene: Hs.591366

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