MYC (Ab-358) Antibody

Datasheet
Code CSB-PA930453
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P01106
Image
  • Western blot analysis of extracts from HL60, MCF and K562 cells using Myc(Ab-358) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Ab-358) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around aa.356~360 (R-R-T-H-N) derived from Human Myc.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total Myc protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200
Relevance Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The expression correlates with the proliferation state of the cells. Transcription is repressed in quiescent or terminally differentiated cells. Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. Myc therefore is an important positive regulator of cell growth and proliferation. Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression. Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates

Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias c-myc
Immunogen Species Homo sapiens (Human)
Target Names MYC
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis
Involvement in disease Burkitt lymphoma (BL)
Subcellular Location Nucleus, nucleoplasm, Nucleus, nucleolus
Database Links

HGNC: 7553

OMIM: 113970

KEGG: hsa:4609

STRING: 9606.ENSP00000367207

UniGene: Hs.202453

Pathway ErbB signaling pathway
Hippo signaling pathway
Jak-STAT signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Cell cycle
Cellular senescence
Signaling pathways regulating pluripotency of stem cells

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