Human c-myc Oncogene product,c-myc ELISA Kit

Code CSB-E09260h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01106
Lead Time 3-5 working days
Abbreviation MYC
Protein Biological Process 1 Transcription/Transcription regulation
Target Name v-myc myelocytomatosis viral oncogene homolog (avian)
Alias MRTL, bHLHe39, c-Myc, avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|myc-related translation/localization regulatory factor|v-myc avian myelocytomatosis viral oncogene homo
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt s lymphomas, suggesting its importance in the normal function of this gene.
HGNC 1052
RGD 621786
MGI 108092
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human c-myc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %87-99
1:2Average %95
Range %91-101
1:4Average %92
Range %88-96
1:8Average %90
Range %86-97
Recovery
The recovery of human c-myc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-96
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.404 2.453 2.429 2.341
101.587 1.575 1.581 1.493
51.084 1.066 1.075 0.987
2.50.658 0.699 0.679 0.591
1.250.456 0.487 0.472 0.384
0.6250.251 0.245 0.248 0.160
0.3120.161 0.169 0.165 0.077
00.087 0.089 0.088  
Still Have Questions? Leave a Message or Start an on-line Chat
Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis
Involvement in disease Burkitt lymphoma (BL)
Subcellular Location Nucleus, nucleoplasm, Nucleus, nucleolus
Database Links

HGNC: 7553

OMIM: 113970

KEGG: hsa:4609

STRING: 9606.ENSP00000367207

UniGene: Hs.202453

Pathway ErbB signaling pathway
Hippo signaling pathway
Jak-STAT signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Cell cycle
Cellular senescence
Signaling pathways regulating pluripotency of stem cells

Q&A and Customer Reviews

Q&A
Q:

Do you know if this Human c-myc Oncogene product,c-myc ELISA kit (CSB-E09260h) will be able to detect myc tagged proteins, or only the whole myc protein itself? The myc tag sequence would be EQKLISEEDL.

A:
Thanks for your inquiry.
CSB-E09260h
This kit detects c-myc Oncogene product. It is designed against full length.
Pls refer to: https://www.uniprot.org/uniprot/P01106.
Myc tagged protein you mentioned (https://en.wikipedia.org/wiki/Myc-tag)is a kind of tag protein.
Sorry we didn't do epitope mapping before, so we are not clear if the antibody in the kit can recognize “EQKLISEEDL”or not.
Customer Reviews

Related Products

MYC Antibodies

MYC Antibodies for Homo sapiens (Human)

MYC Antibodies for Human

MYC Proteins

MYC Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.