Human c-myc Oncogene product,c-myc ELISA Kit

Instructions
Code CSB-E09260h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name v-myc myelocytomatosis viral oncogene homolog (avian)
Alternative Names MRTL, bHLHe39, c-Myc, avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|myc-related translation/localization regulatory factor|v-myc avian myelocytomatosis viral oncogene homo
Abbreviation MYC
Uniprot No. P01106
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human c-myc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %87-99
1:2Average %95
Range %91-101
1:4Average %92
Range %88-96
1:8Average %90
Range %86-97
Recovery
The recovery of human c-myc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-96
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.404 2.453 2.429 2.341
101.587 1.575 1.581 1.493
51.084 1.066 1.075 0.987
2.50.658 0.699 0.679 0.591
1.250.456 0.487 0.472 0.384
0.6250.251 0.245 0.248 0.160
0.3120.161 0.169 0.165 0.077
00.087 0.089 0.088  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

Do you know if this Human c-myc Oncogene product,c-myc ELISA kit (CSB-E09260h) will be able to detect myc tagged proteins, or only the whole myc protein itself? The myc tag sequence would be EQKLISEEDL.

A:
Thanks for your inquiry.
CSB-E09260h
This kit detects c-myc Oncogene product. It is designed against full length.
Pls refer to: https://www.uniprot.org/uniprot/P01106.
Myc tagged protein you mentioned (https://en.wikipedia.org/wiki/Myc-tag)is a kind of tag protein.
Sorry we didn't do epitope mapping before, so we are not clear if the antibody in the kit can recognize “EQKLISEEDL”or not.
 Customer Reviews

Target Data

Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis
Involvement in disease Burkitt lymphoma (BL)
Subcellular Location Nucleus, nucleoplasm, Nucleus, nucleolus
Database Links

HGNC: 7553

OMIM: 113970

KEGG: hsa:4609

STRING: 9606.ENSP00000367207

UniGene: Hs.202453

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