PAG 1 Antibody

Code CSB-PA644917LA01BO
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Raji whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate
    All lanes: Pregnancy-associated glycoprotein 1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 43 kDa
    Observed band size: 43 kDa

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Product Details

Description
PAG 1 polyclonal antibody CSB-PA644917LA01BO was produced in the rabbit immunized by using the Recombinant Bovine Pregnancy-associated glycoprotein 1 protein (54-380AA) as the immunogen. The target protein PAG 1 appears to be proteolytically inactive. It is expressed in trophoblast and placental tissue. It is produced specifically in the invasive binucleate cells of the placenta. It becomes detectable in maternal serum soon after implantation.
This PAG 1 Antibody was tested in the ELISA and WB. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the PAG 1 proteins of bovine or human or rat-origin and may be used to detect the endogenous levels of PAG 1 protein.
Uniprot No. Q29432
Alternative Names Pregnancy-associated glycoprotein 1 antibody; PAG 1 antibody; EC 3.4.23.- antibody; Pregnancy-specific protein B antibody; PSP-B antibody
Raised in Rabbit
Species Reactivity Bovine, Human, Rat
Immunogen Recombinant Bovine Pregnancy-associated glycoprotein 1 protein (54-380AA)
Immunogen Species Bos taurus (Bovine)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Appears to be proteolytically inactive.
Gene References into Functions
  1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
  2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
  3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
  4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
Subcellular Location Secreted, extracellular space
Protein Families Peptidase A1 family
Tissue Specificity Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
Database Links

KEGG: bta:281964

STRING: 9913.ENSBTAP00000025593

UniGene: Bt.15401

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