Recombinant Bovine Pregnancy-associated glycoprotein 1

Code CSB-BP644917BO
Size US$1888Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Uniprot No. Q29432
Research Area Others
Alternative Names Pregnancy-associated glycoprotein 1; PAG 1; EC 3.4.23.-; Pregnancy-specific protein B; PSP-B
Species Bos taurus (Bovine)
Source Baculovirus
Expression Region 54-380aa
Target Protein Sequence RGSNLTTHPLRNIKDLVYMGNITIGTPPQEFQVVFDTASSDLWVPSDFCTSPACSTHVRFRHLQSSTFRLTNKTFRITYGSGRMKGVVVHDTVRIGNLVSTDQPFGLSIEEYGFEGRIYDGVLGLNYPNISFSGAIPIFDKLKNQRAISEPVFAFYLSKDEREGSVVMFGGVDHRYYEGELNWVPLIQAGDWSVHMDRISIERKIIACSDGCKALVDTGTSDIVGPRRLVNNIHRLIGAIPRGSEHYVPCSEVNTLPSIVFTINGINYPVPGRAYILKDDRGRCYTTFQENRVSSSTETWYLGDVFLRLYFSVFDRGNDRIGLARAV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 38.7 kDa
Protein Length Full Length of Mature Protein
Tag Info C-terminal 9xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Appears to be proteolytically inactive.
Gene References into Functions
  1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
  2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
  3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
  4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
Subcellular Location Secreted, extracellular space
Protein Families Peptidase A1 family
Tissue Specificity Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
Database Links

KEGG: bta:281964

STRING: 9913.ENSBTAP00000025593

UniGene: Bt.15401

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