Product Details

Uniprot No.

P51946

Target Names

Alternative Names

6330408H09Rik antibody; AI661354 antibody; AV102684 antibody; AW538719 antibody; CAK antibody; CAK complex subunit antibody; ccnh antibody; CCNH_HUMAN antibody; CDK activating kinase antibody; CDK activating kinase complex subunit antibody; Cyclin dependent kinase activating kinase antibody; cyclin dependent kinase activating kinase complex subunit antibody; Cyclin H antibody; Cyclin-H antibody; CyclinH antibody; MO15 associated protein antibody; MO15-associated protein antibody; p34 antibody; p36 antibody; p37 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from Human Cyclin H around the phosphorylation site of T315.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:10000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Gene References into Functions

Expressions of components of the CAK complex, CDK7, MAT1, and Cyclin H are elevated in breast cancer. PMID: 27301701
Our results indicated that CCNH/CDK7-CtBP2 axis may augment ESCC cell migration, and targeting the interaction of both may provide a novel therapeutic target of esophageal squamous cell carcinoma . PMID: 25820824
SNPs in CCNH and ABCG2 can modulate the development of severe oxaliplatin-induced peripheral neuropathy. PMID: 24351404
Hemodynamic forces modulate EC proliferative phenotype through the miR-23b/CAK/cyclin H pathway. PMID: 24855060
When considering all thyroid cancer (DTC) cases, only rs2230641 (CCNH) was associated with DTC risk. PMID: 23982724
CCNH and CDK7 may play an important role in the tumorigenesis and development of esophageal squamous cell carcinoma. PMID: 23456497
High Cyclin H is associated with gastrointestinal stromal tumours. PMID: 20598140
The expression of cyclin H and CDK7 protein in proliferating hemangiomas was significantly higher than that in involuting hemangiomas and normal skin tissues. PMID: 18950027
The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H. PMID: 12140753
Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG glioma cells, supporting its role in cell signaling PMID: 15695176
In response to ATRA, PML/RARalpha is dissociated from CAK, leading to MAT1 degradation, G1 arrest, and decreased CAK phosphorylation of PML/RARalpha PMID: 16935935
Retinoic-acid-induced RAR-CAK signaling events appear to proceed intrinsically during granulocytic development of normal primitive hematopoietic cells. ALDH-governed RA availability may mediate this process by initiating RAR-CAK signaling. PMID: 17628022
These results suggested that genetic variants in CAK genes, Cdk7, cyclin H, MAT1, might modulate the risk of lung cancer in a gene-gene interaction mode, which consist to the biochemical interaction of corresponding proteins. PMID: 17707548
Our study demonstrates the independent prognostic value of cyclin H expression in diffuse large B-cell lymphoma and proposes its use as a prognostic marker. PMID: 18400256
TFIIH changes subunit composition in response to DNA damage. The CAK is released from the core during nucleotide excision repair (NER). PMID: 18614043

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Subcellular Location

Nucleus.

Protein Families

Cyclin family, Cyclin C subfamily

Database Links

HGNC: 1594

OMIM: 601953

KEGG: hsa:902

STRING: 9606.ENSP00000256897

UniGene: Hs.292524

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Code	CSB-PA070202
Size	US$119
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Phospho-CCNH (T315) Antibody

Product Details

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Target Background