Phospho-H2AFX (S139) Antibody

Code CSB-PA010097OA139phHU
Size US$319
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
    All lanes: H2AFX antibody at 1.8μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA010097OA139phHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFX (CSB-PA010097OA139phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) H2AFX Polyclonal antibody
Uniprot No. P16104
Target Names H2AFX
Alternative Names H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_HUMAN antibody; Histone H2A.X antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Phospho-Ser (139) derived from Human Histone H2AX
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:50-1:500
IF 1:1-1:10
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Gene References into Functions
  1. ZNF506-dependent positive feedback loop regulates H2AX signaling after DNA damage. PMID: 30013081
  2. This study confirms that H2AFX variants are associated with an increased risk of BC. The above-reported sequence variants of MRE11 genes may not constitute a risk factor of breast cancer in the Polish population. PMID: 29678143
  3. gamma-irradiation also decreased the number of cells in the G1 phase, characterized by no interaction between H3S10ph and gammaH2AX. PMID: 30096372
  4. The topology of clusters of gammaH2AX foci can be categorized depending on the distance to heterochromatin. The here presented new method opens up new possibilities to categorize spatial organization of point patterns by parameterization of topological similarity. PMID: 30072594
  5. this study suggests that individual and co-expression pattern of nuclear oxidized-PTP and gamma-H2AX might be used as a prognostic marker of gastric carcinoma PMID: 30126387
  6. Low pH2AX expression is associated with mouth Cancer. PMID: 30275188
  7. Results show that the H2AX 3'U TR is targeted by miR328 and its expression inhibited in osteosarcoma cells under radiation conditions. PMID: 29207178
  8. The results propose a model in which Aurora B-mediated H2AX-phosphorylated serine 121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation. PMID: 27389782
  9. Data indicate that nuclear H2A histone family, member X protein (gammaH2AX) expression is positively associated with the programmed death-ligand 1 (PD-L1) expression in lung squamous cell carcinoma. PMID: 29275316
  10. phosphorylated histone H2AX was predictive of disease progression epithelial dysplasia of the oral cavity. PMID: 28543539
  11. Gamma-H2AX, phosphorylated KAP-1 and 53BP1 play an important role in the repair of heterochromatic radon-induced DNA double-strand breaks. PMID: 27922110
  12. in a series of human biopsies, non-metastatic SCCs displayed a higher degree of chromosomal alterations and higher expression of the S phase regulator Cyclin E and the DNA damage signal gammaH2AX than the less aggressive, non-squamous, basal cell carcinomas. However, metastatic Squamous cell carcinoma lost the gammaH2AX signal and Cyclin E, or accumulated cytoplasmic Cyclin E. PMID: 28661481
  13. We found that phosphorylation of histone H2AX on Ser139 (gamma-H2AX), a biomarker of DSBs, and phosphorylation of ATM at Ser1981, Chk2 at Thr68, and p53 at Ser15, part of signaling pathways associated with DSBs, are elevated in these cells. PMID: 28388353
  14. number of gammaH2AX foci did not significantly change following cardiac MR (median foci per cell pre-MR = 0.11, post-MR = 0.11, p = .90), but the number of 53BP1 foci significantly increased following MR PMID: 29309426
  15. Study provides evidence that phosphorylated H2AX binds to the promoter of miR-3196 and regulate its expression leading to lung cancer cell apoptosis. PMID: 27780918
  16. there may not be a link between low level phosphorylation gammaH2AX sites and double-strand DNA breaks in cells exposed to topoisomerase I or II inhibitors PMID: 27391338
  17. Residual gammaH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells. PMID: 29165316
  18. miR-24-mediated knockdown of H2AX may be a novel negative regulator of mitochondrial function and insulin signaling. PMID: 28386126
  19. suggest that pH2AX alone or better in combination with MAP17 may become a novel and valuable prognostic biomarker for patients with laryngeal carcinoma treated with preservation approaches. PMID: 27166270
  20. The findings demonstrate that RNF168 couples PALB2-dependent homologous recombination to H2A ubiquitylation to promote DNA repair and preserve genome integrity. PMID: 28240985
  21. Data show that co-treated with vincristine and XL019, a inhibitor of JAK2 and P-glycoprotein (P-gp), up-regulated expression of p21 and phosphorylated H2A histone family, member X (pH2AX). PMID: 29187454
  22. The bile acid receptor TGR5, inducible nitric oxide synthase (iNOS) and gamma-histone family 2A variant (gamma-H2AX) are up-regulated. PMID: 27247425
  23. Co-localization of gammaH2AX and 53BP1 indicates promotion of (in)effective nonhomologous end-joining repair mechanisms at sites of DSB. Moreover, gammaH2AX/53BP1 foci distribution presumably reveals a non-random spatial organization of the genome in MDS and AML. PMID: 28359030
  24. Cyclin F-mediated degradation of SLBP limits H2A.X accumulation and apoptosis upon genotoxic stress in G2 cell cycle checkpoint. PMID: 27773672
  25. study demonstrates that the individual and combined expression patterns of the DDR molecules PARP1, gammaH2AX, BRCA1, and BRCA2 could be predictive of the prognosis of STS patients and suggests that controlling the activity of these DDR molecules could be employed in new therapeutic stratagems for the treatment of STS PMID: 27643881
  26. Further analysis suggested that H2AX, a PARP-1 protein interaction partner, was coordinated with PARP-1 in hepatocellular carcinoma tumorigenesis. Overall, some new characteristics of PARP-1 expression were noted in the Zhuang population. PARP-1 is a novel promising diagnostic marker for hepatocellular carcinoma in the Southern Chinese Zhuang population PMID: 28714367
  27. we found that gamma-H2AX foci at chromosome boundaries after carbon-ion irradiation contain DNA double strand breaks undergoing DNA-end resection, which promotes repair utilizing microhomology mediated end-joining during translocation. PMID: 27113385
  28. this study demonstrates an early DDR defect of attenuated gammaH2AX signals in G0/G1 phase HGPS cells and provides a plausible connection between H3K9me3 loss and DDR deficiency. PMID: 27907109
  29. Data indicate an important role for histone H2A.X (H2AX) Tyr39 phosphorylation in gamma-H2A.X formation and cancer progression. PMID: 27813335
  30. we suggest that the XAB2 complex mediates DNA damage response events important for the end resection step of homologous recombination , and speculate that its adjacent-localization relative to double-strand break marked by gH2AX is important for this function PMID: 27084940
  31. the epithelial-mesenchymal transition-related transcription factor Twist1 cooperates with Slug to regulate EMT upon H2A.X Loss. PMID: 27315462
  32. Upon DNA damage, an increase in the levels of chromatin bound motor protein nuclear myosin 1 (NM1) ensues, which appears to be functionally linked to Upsilon-H2AX signaling. PMID: 27365048
  33. TRAF6 and H2AX overexpression and gammaH2AX-mediated HIF1alpha enrichment in the nucleus of cancer cells lead to overactivation of HIF1alpha-driven tumorigenesis, glycolysis and metastasis. PMID: 27918549
  34. gammaH2AX, claimed to be a marker of DNA double-strand breaks, was found in cell extracts of HeLa cells at elevated temperature vs. 37.0 degrees C, and these gammaH2AX signals were intensified in the presence of 3-aminobenzamide, a PARP inhibitor. PMID: 27262441
  35. Data provide evidence that the acetylation of H2AX at Lys5 by TIP60 is required for the (ADPribosyl) ation activity and the dynamic binding of PARP-1 to chromatin after the induction of DNA damage. PMID: 26976643
  36. data cannot finally exclude H2AX methylation of SUV39H2 in cells, additional experimental evidence is required to validate this claim. PMID: 27177470
  37. This review outlines the role of gamma-H2AX in cell cycle, and its formation as a result of DNA damage. We investigate the role of gamma-H2AX formation in several cancer types and its correlation with other prognostic factors, and we try to find out whether it fulfills the requirements for its establishment as a classical cancer prognostic factor PMID: 28351323
  38. this study identified histone H2AX as an antigen of systemic lupus erythematosus by comparing highly ranked genes from all the built network-derived gene lists, which was confirmed the with real-world clinical samples PMID: 27226232
  39. dyserythropoiesis was increased in MDS patients with the deletion of chromosome 11q23, where H2AX is located. Although loss of H2AX did not affect the early stage of terminal erythropoiesis, enucleation was decreased. PMID: 26791933
  40. the formation of 53BP1, gammaH2AX foci and their co-localization induced by gamma-rays (2, 5, 10, 50, 200 cGy) in human lymphocytes, was analyzed. PMID: 26243567
  41. 5-Hydroxymethylcytosine (5hmC) accumulates at DNA damage foci and colocalizes with major DNA damage response proteins 53BP1 and gH2AX, revealing 5hmC as an epigenetic marker of DNA damage. PMID: 26854228
  42. Anacardic acid sensitizes prostate cancer cells to radiation therapy by repressing H2AX expression. PMID: 26884865
  43. Results reveal a pathway controlled by ATM, SIRT6, and SNF2H to block HUWE1, which stabilizes H2AX and induces its incorporation into chromatin only when cells are damaged. PMID: 26711340
  44. Gene expression analysis identified deregulation of histone H2A and H2B genes in all four cell lines ;histone pathways are associated with epirubicin resistance PMID: 26852132
  45. kinetics of the accumulation of selected DNA repair-related proteins is protein specific at locally induced DNA lesions, and that the formation of gH2AX- and NBS1-positive foci, but not 53BP1-positive NBs, is cell cycle dependent in HeLa cells PMID: 26482424
  46. The interaction of MDC1 with RNF8, but not with ATM requires WRAP53beta, suggesting that WRAP53beta facilitates the former interaction without altering phosphorylation of MDC1 by ATM. PMID: 26734725
  47. the interaction of 53BP1 with gammaH2AX is required for sustaining the 53BP1-dependent focal concentration of activated ATM that facilitates repair of DNA double-strand breaks in heterochromatin in G1. PMID: 26628370
  48. X-rays induce prolonged and ATM-independent persistence of gammaH2AX foci in human gingival mesenchymal stem cells PMID: 26314960
  49. Cell levels of gammaH2Ax define the G2 phase of the cell cycle. PMID: 26317799
  50. The study shows higher expression of gamma-H2AX and 53BP1 foci in rectal cancer patients compared with healthy individuals. Yet the data in vitro were not predictive in regard to the radiotherapy outcome. PMID: 26541290
  51. overexpression of TIPRL promotes phosphorylation of H2AX, and increases gamma-H2AX positive foci in response to DNA damage, whereas knockdown of TIPRL inhibits gamma-H2AX phosphorylation PMID: 26717153
  52. For both, gamma-H2AX and 53BP1, the cellular focus number as well as the percentage of positive cells did not differ between patients with clinically isolated syndrome/early relapsing-remitting multiple sclerosis and healthy controls. PMID: 26820970
  53. silencing or removing H2A.X induces mesenchymal-like characteristics including activation of EMT transcription factors, Slug and ZEB1, in HCT116 human colon cancer cells. PMID: 26876487
  54. All the 17 common single-nucleotide polymorphisms located within the 3'UTR of H2AFX gene were analyzed for putative miRNA-binding sites. PMID: 25738604
  55. link gamma-H2AX to transcription, assigning a new function for this DNA damage marker PMID: 26045162
  56. These findings elucidate deeply and extensively the mechanism of RNF8/RNF168 and USP11 to maintain the proper status of ubiquitylation gammaH2AX to repair double strand breaks. PMID: 26507658
  57. H2AX signal kinetics and phosphorylation after irradiation and its link to double-stranded DNA breaks pattern. PMID: 26067661
  58. gammaH2AX levels were higher in the sperm of male infertility patients than in healthy men. PMID: 26158906
  59. pH2AX may be used to detect epithelial ovarian cancer at an early stage and identify women at higher risk for relapse. PMID: 26191270
  60. Quinazolinone analogue HMJ-38-provoked DNA damage stress in HUVECs via activation of gammaH2A.X/DNA-PK/GSK-3beta signaling. PMID: 24064905
  61. Apoptosis induction was found to be mitochondria mediated, involving increased free intracellular Ca(2+), ROS, and upregulation of soluble histone H2AX. PMID: 26026585
  62. H2AX phosphorylation at Ser139 in human K562 cells is regulated by p38 and JNK, and is essential for resveratrol-induced apoptosis. PMID: 25619392
  63. Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168. PMID: 25695757
  64. These findings suggest age, race, ethnicity and alcohol use influence DNA double strand breaks gamma-H2AX repair kinetics. PMID: 25794041
  65. Increased gammaH2AX expression is associated with rectal cancer. PMID: 25889915
  66. The phosphorylation of H2AX and the formation of gammaH2AX foci induced by ionizing radiation (IR), are prevented by VRK1 depletion and are rescued by kinaseactive, but not kinase-dead, VRK1. PMID: 25923214
  67. endogenous gamma-H2AX is associated with short telomeres, which might offer a specific target for therapy for triple-negative breast cancer patients PMID: 25808442
  68. Treatment with the DNA-dependent protein kinase (DNA-PK) inhibitor significantly reduced gamma-H2AX foci formation (P<0.05) following acute radiation exposure in the radioresistant sub-clones, compared with the parental control cells PMID: 25815686
  69. DNA double-strand breaks by Cr(VI) are targeted to euchromatin and cause ATR-dependent phosphorylation of histone H2AX and its ubiquitination. PMID: 25288669
  70. Data suggest that the mechanism of gamma-H2AX on the angiogenic activity of hepatocellular carcinoma (HCC) might go through EGFR/HIF-1alpha/VEGF pathways under hypoxic conditions. PMID: 25537504
  71. Following alpha particle irradiation pan-nuclear phosphorylation of H2AX and ATM, but not DNA-PK and 53BP1, was observed throughout the nucleus. PMID: 26116906
  72. 46 kinases involved either directly or indirectly in H2AX phosphorylation. PMID: 25894690
  73. The review illustrates the critical role of Arf/p53 mutations-dependent H2AX downregulation in the establishment of a growth-arrested cellular state. PMID: 22754379
  74. 3 SNPs (rs10790283 G > A, rs604714 C > A and rs7759 A > G) were significantly associated with DNA damage levels. Significant interactions were observed between certain genetic polymorphisms and PM2.5-modulated DNA damage levels. PMID: 26073471
  75. A high p-H2AX expression in CRC tissues is associated with a more malignant cancer behavior, as well as poor patient survival. p-H2AX may, therefore, be an independent prognostic predictor for CRC, as well as a potential therapeutic target. PMID: 25862913
  76. PhosphoChk1 and H2AX are useful biomarkers for ATR inhibition using a variety of immuno-detection methods, but timing may be critical PMID: 25459351
  77. Intensive oxidative stress induces sustained high levels of gamma-H2AX and p53 PMID: 25293814
  78. while PARP1 over-expression was detected in blasts not responsive to olaparib, phosphorylation of the histone H2AFX (gammaH2AX) was associated with drug sensitivity. PMID: 25483710
  79. H2AX methylation plays a role in the regulation of gamma-H2AX abundance in cancer PMID: 25487737
  80. we were able to show that miR-138 potently inhibits H2AX expression PMID: 25699650
  81. Characterization of functional impact of rs643788, rs8551, rs7759 and rs2509049 upstream variants in H2AFX distal promoter. PMID: 25847270
  82. PP2A holoenzyme containing B56 is responsible for the dephosphorylation of gamma-H2AX and regulation of DNA repair of double strand breaks induced by camptothecin. PMID: 25772433
  83. Studies indicate the importance of the histone variant H2AX in a multitude of biological processes . PMID: 25712102
  84. The expression of nuclear gamma-H2AX might be closely related to the prognosis of laryngeal squamous cell carcinoma . PMID: 25043086
  85. Single-stranded DNA arisen from stalled replication forks can cause the ATR-mediated induction of phosphorylated H2AX. PMID: 24682951
  86. gammaH2AX expression was increased in papillary thyroid carcinoma patients, correlating negatively with TNM stage and lymph node metastasis. PMID: 25651738
  87. hyperosmotic stress-activated Plk3 elicited gammaH2AX. PMID: 25202016
  88. H2AX phosphorylation regulated by p38 is involved in Bim expression and apoptosis in chronic myelogenous leukemia cells induced by imatinib. PMID: 24830786
  89. The study reports the discovery of a proteolytic product of histone H2A in chronic lymphocytic leukemia and the myeloid THP-1 cell line. PMID: 24871368
  90. The acidic patch functions within the nucleosome as nucleosomes containing a mutated acidic patch exhibit defective H2A/H2AXub by RNF168 and RING1B/BMI1 in vitro PMID: 24603765
  91. More residual foci are observed in RC10.1 than in RKO, indicating that decay of gamma-H2AX foci correlates with p53 functionality and PLDR in RKO cells. PMID: 24363165
  92. Taken together, the results suggested that USP3 is a negative regulator of ubiquitination signaling, counteracting RNF168- and RNF8-mediated ubiquitination. PMID: 24196443
  93. this study demonstrated that the AKLIDES system can be used as an efficient automatic screening tool for gammaH2AX foci analysis by providing new evaluation features and facilitating the identification of drugs which induce or modulate DNA damage PMID: 24009179
  94. Activation of H2AX and ATM in varicella-zoster virus infected cells is associated with expression of VZV ORF61 and ORF63. PMID: 24606682
  95. EBNA3C downregulates H2AX expression at the protein and transcript levels in epithelial cells, B cells, and Epstein-Barr virus-transformed lymphoblastoid cell lines. PMID: 24429368
  96. Kaposi's sarcoma-associated herpesvirus induces the ATM and H2AX DNA damage response early during de novo infection of primary endothelial cells, which play roles in latency establishment. PMID: 24352470
  97. The findings suggest that active DNA replication is accompanied with the specific localization of H2AX for efficient damage repair or replication-fork stabilization in actively transcribed regions. PMID: 24163101
  98. Knockdown of H2AX strongly suppressed apoptosis of A549 cells. PMID: 23793869
  99. ZNF668 knockdown reduces Tip60-H2AX interaction and impairs ionizing radiation-induced histone H2AX hyperacetylation. PMID: 23777805
  100. An increased level of gammaH2AX occurred in mitotic cells, and this increase was attenuated by DNA-PKcs inactivation or Chk2 depletion, but not by ATM inhibition. PMID: 24021642

Show More

Hide All

Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

HGNC: 4739

OMIM: 601772

KEGG: hsa:3014

STRING: 9606.ENSP00000364310

UniGene: Hs.477879

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1