Phospho-H2AFX (S139) Antibody

Datasheet
Code CSB-PA010097OA139phHU
Size US$319
Uniprot No. P16104
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
    All lanes: H2AFX antibody at 1.8μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA010097OA139phHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFX (CSB-PA010097OA139phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Immunogen Peptide sequence around site of Phospho-Ser (139) derived from Human Histone H2AX
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF, ChIP; Recommended dilution: WB:1:50-1:500, IF:1:1-1:10
Relevance Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H2AX (H2a/x) (Histone H2A.X), H2AFX, H2AX
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names H2AFX
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

HGNC: 4739

OMIM: 601772

KEGG: hsa:3014

STRING: 9606.ENSP00000364310

UniGene: Hs.477879

Pathway Necroptosis

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