Phospho-NR3C1 (S226) Antibody

Code CSB-PA008994
Size US$167
  • Western Blot analysis of HELA 293T cells using Phospho-GR (S226) Polyclonal Antibody
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Product Details

Uniprot No. P04150
Target Names NR3C1
Alternative Names GCCR antibody; GCR antibody; GCR_HUMAN antibody; GCRST antibody; glucocorticoid nuclear receptor variant 1 antibody; Glucocorticoid receptor antibody; GR antibody; GRL antibody; Grl1 antibody; nr3c1 antibody; Nuclear receptor subfamily 3 group C member 1 antibody; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human GR around the phosphorylation site of S226.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Receptor for glucocorticoids (GC)
Gene References into Functions
  1. relaxin-GR signaling has role in hepatocellular protection against ischemia-reperfusion stress in liver transplantation PMID: 29350771
  2. Bcl1 G/G polymorphism of glucocorticoid receptor gene is associated with bronchial asthma complicated by obesity. PMID: 30480407
  3. topical mevastatin accelerates wound closure by promoting epithelialization via multiple mechanisms: modulation of GR ligands and induction of the long noncoding RNA Gas5, leading to c-Myc inhibition. PMID: 29158265
  4. alpha-Viniferin (KCV) inhibits the activation of glucocorticoid receptor (GR) signaling pathway in non-androgen-dependent Prostate cancer (PCa) cells. KCV induces cancer cell apoptosis through AMP-Activated Protein Kinases-mediated activation of autophagy, and inhibits GR expression in castration-resistant prostate cancer(CRPC). PMID: 29904891
  5. The genotypes for the NR3C1 polymorphisms in patients and controls were distributed as follows: rs6191 TT 37 : 56, GT 178 : 36, GG 332 : 609; rs6196 AA 483 : 905, AG 66 : 118, GG 2 : 4; rs10482614 GG 493 : 916, AG 61 : 108, AA 1 : 4; and rs72557310 AG 27 : 65, GG 3 : 0, AA 525 : 964. There were no significant differences in genotype frequency or in allele distributions between cases and controls. PMID: 29381656
  6. Glucocorticoid receptor positively regulates transcription of FNDC5 in the liver. PMID: 28240298
  7. Polymorphisms in NR3C1 gene is associated with sensitivity to glucocorticoids and it may contribute to the glucose abnormality for Acute Lymphoblastic Leukemia. PMID: 29802709
  8. NR3C1 methylation moderates the effect of maternal support during stress on anxious attachment development 18 months later. More stressed children who experienced less maternal support reported increased anxious attachment when their NR3C1 gene was highly methylated. This effect could not be explained by children's level of psychopathology. PMID: 29058930
  9. Meta-analysis showed that homozygous mutation of NR3C1 rs41423247 was associated with Depression. PMID: 30278546
  10. This review focuses on the earlier findings on the pathophysiology of GR signaling and presents criteria facilitating identification of novel NR3C1 mutations in selected patients. [review] PMID: 29685454
  11. study indicates that GR genetic polymorphisms may play a major role in the pathogenesis and development of systemic lupus erythematosus PMID: 28984075
  12. The BclI NR3C1 polymorphisms were significantly associated with asthma in adults. (Meta-analysis) PMID: 29729712
  13. Here we show genome-wide that blocked GBR generally require CHD9 and BRM for GR occupancy in contrast to GBR that are not blocked by Hic-5. Hic-5 blocked GBR are enriched near Hic-5 blocked GR target genes but not near GR target genes that are not blocked by Hic-5. PMID: 29738565
  14. There was no significant association between different genotypes and alleles of Glucocorticoid Receptor of rs6195, rs6189/rs6190 variants, and response to fluoxetine (p=0.213 and 0.99, respectively). PMID: 28641498
  15. NR3C1 gene polymorphisms are significantly associated with the response to glucocorticoids. PMID: 29207898
  16. There is no clear evidence that the analysed NR3C1 allelic variants confer a risk for developing systemic autoimmune diseases although the minor G allele of rs41423247 may be protective among Caucasians (review and meta-analysis). PMID: 29526633
  17. Analyses demonstrated a trend in the association between maternal trait anxiety and depression symptoms with placental gene expression of NR3C1. We found a significant interaction with maternal ethnicity. In Caucasians only, prenatal trait anxiety and depressive symptoms were associated with an increase in placental NR3C1 expression, and prenatal life events were associated with a down regulation of HSD11B2 PMID: 29100173
  18. We genotyped 10 single nucleotide polymorphisms (SNPs) on the NR3C1 gene (rs10482682, rs33389, rs10482633, rs10515522, rs2963156, rs4128428, rs9324918, rs41423247, rs6189, rs10052957).Haplotype analyses revealed significant effects of NR3C1 (p = 0.011) on cortisol stress response. Neither NR3C1 haplotype nor NR3C2 haplotype was associated with reasoning abilities. PMID: 29100174
  19. In this study, we described the cellular localization of the glucocorticoid receptor in the human adult and fetal testis and provided evidence of an association between semen quality and a genetic polymorphism BclI (rs41423247) in the NR3C1 gene. PMID: 28992366
  20. Results indicate that maltreated children evidence higher baseline levels of NR3C1 methylation, significant decreases in methylation over time, and then at follow-up, lower levels of methylation, relative to nonmaltreated preschoolers. PMID: 29162170
  21. children with early onset maltreatment evidence significant hypermethylation compared to nonmaltreated children. Also, hypermethylation of NR3C1 is linked with a number of negative child outcomes including greater emotional lability-negativity, higher levels of ego undercontrol, more externalizing behavior, and greater depressive symptoms. PMID: 29162187
  22. Study evaluated whether associations between early adversity and brain responses to dynamic facial expressions in early adulthood varied as a function of regional differences in the expression of NR3C1. Strongest associations between adversities and BOLD response to fearful faces were in brain regions with higher NR3C1 mRNA expression levels. Highest expression of NR3C1 is found in occipital and lowest in temporal regions PMID: 28612935
  23. Study define a distinct GRgamma driven signaling network including identification of GRgamma specific subcellular trafficking, target gene selection, and engagement of interacting proteins. Both transcriptome, and protein interactome data suggested a role in for GRgamma in directing mitochondrial function, and indeed GRgamma expression increased mitochondrial mass, basal respiration, and ATP generation. PMID: 27226058
  24. Studied single nucleotide polymorphisms in human glucocorticoid receptor (NR3C1) gene with regard to susceptibility in high-altitude pulmonary edema (HAPE) in Han Chinese population. PMID: 29587872
  25. In patients with adrenal incidentalomas, a 5% prevalence of heterozygous NR3C1 mutations was discovered. PMID: 29444898
  26. This study demonstrated that NR3C1 expression levels are related to major depressive disorder and conjunctly mediate the effect of childhood maltreatment history on the risk of developing major depressive disorder. PMID: 28384542
  27. This study demonstrated that increased methylation of glucocorticoid receptor gene promoter 1F in peripheral blood of patients with generalized anxiety disorder. PMID: 28292649
  28. This study suggests that SNPs in the NR3C1 gene may influence BDNF levels in crack cocaine addiction. PMID: 28237884
  29. We identified a molecular signature of secreted proteins associated with AA ultraresponsiveness and sustained AR/GR signaling upon AA resistance in intermediate or minimal responders. These data will inform development of noninvasive biomarkers predicting AA response and suggest that further inhibition along the AR/GR signaling axis may be effective only in AA-resistant patients who are intermediate or minimal responders PMID: 27993966
  30. A Tri-Nucleotide Pattern in a 3' UTR Segment Affects The Activity of a Human Glucocorticoid Receptor Isoform PMID: 27660999
  31. Association Between N363S and BclI Polymorphisms of the Glucocorticoid Receptor Gene (NR3C1) and Glucocorticoid Side Effects During Childhood Acute Lymphoblastic Leukemia Treatment. PMID: 28179212
  32. genetic association studies in population in Brazil: Data suggest that an SNP in NR3C1 (A3669G) is associated with appetite regulation and food preferences; here, adolescents carrying A3669G variant exhibited decreased comfort food intake. PMID: 28400302
  33. Results provide evidence for an association between the NR3C1-rs41423247 SNP and depression: C minor allele of rs41423247 increased depressive symptoms during early abstinence of women with crack cocaine addiction, but it did not have effects over detoxification treatment. A slight effect of CC genotype was shown at late abstinence phase. C allele of this SNP was associated to an increased number of rehospitalizations. PMID: 27397864
  34. There was no significant interaction between NR3C1 and stressful life events with respect to alcohol use/misuse. PMID: 26751645
  35. Dehydroepiandrosterone (DHEA) and cortisol modulate SRSF9 and SRSF3 in a different way and data suggest that the anti-glucocorticoid effect of DHEA, among other mechanisms, is also exerted by modulating the expression of proteins involved in the splicing of the GR pre-mRNA. PMID: 28373129
  36. Association between suicide and altered NR3C1 gene expression in the prefrontal cortex. PMID: 27030168
  37. Results identified three novel heterozygous missense NR3C1 mutations causing glucocorticoid resistance in patients with adrenal incidentalomas without Cushing's syndrome. p.R477S and p.Y478C are located in the DNA binding domain (DBD) of the glucocorticoid receptor (GR) while p.L67P is located in the ligand binding domain of GR. PMID: 27120390
  38. Data show that the 3' UTR of glucocorticoid receptor beta (GRbeta) is regulated by miR144. PMID: 27036026
  39. Except for a slightly higher risk of bronchopulmonary dysplasia (BPD) in carriers of the GRBclI variant, the glucocorticoid receptor gene polymorphisms BclI, N363S, and R23K did not affect neonatal outcome parameters in this large multicenter cohort of Very-Low-Birth-Weight preterm infants. PMID: 27509264
  40. Possible influence of BclI C/G polymorphism (rs41423247) on hippocampal shape and integrity of the parahippocampal subdivision of the cingulum in depression. PMID: 27428087
  41. A woman with glucocorticoid resistance and her mother had a novel p.Arg477Cys (c.1429C>T) mutation in exon 4 of NR3C1, in the 2dzinc finger of the DNA-binding domain. Its 'in silico' functional effect was assessed using pathogenicity prediction software, being characterized as pathogenic. An unrelated patient had a novel p.His588Leufs*5 (c.1762_1763insTTAC) mutation, in exon 6, in the ligand binding domain. PMID: 27211791
  42. NR3C1 as an important gene of the hypothalamic-pituitary-adrenal axis seems to be particularly relevant for the pathophysiology of ADHD combined with comorbid CD. PMID: 27741480
  43. a significant protein-protein interaction between GR and CHOP, (GR-CHOP heterocomplex formation) under endoplasmic reticulum stress conditions, is reported. PMID: 27496643
  44. Childhood Maltreatment and MDD are both associated wit haltered DNA methylation levels in the NR3C1 promoter region, however the location and direction of effects differ between the two exposure.s PMID: 27475889
  45. This study presents evidence of reduced methylation of NR3C1 in association with childhood maltreatment and depressive, anxiety and substance-use disorders in adults. PMID: 27378548
  46. genetic association studies in a racially diverse population in North Carolina: Data suggest that an SNP in NR3C1 (rs6191, G3134T, "glucocorticoid receptor beta") is associated with altered gene expression profile in primary macrophages; minor allele frequency is 74% with a higher prevalence in Caucasian non-Hispanic participants. PMID: 28759007
  47. Decreased DNA methylation of CpG1 of NR3C1 in high-risk infants may allow for increased binding of transcription factors involved in the stress response, repair and regulation of NR3C1. This may ensure healthy growth in high-risk preterm infants over increasing cortisol levels. PMID: 27653086
  48. G-allele was associated with childhood overweight, depressive disorder comorbidity, and diagnostic instability. G-allele carriers reporting childhood overweight showed greater frequency of subjective binge eating and emotional eating. PMID: 27400218
  49. haplotype TAAT of GR might be a protective factor against aggressive behavior, while gene-gene interactions between GR rs1800445 and MR (NR3C2) rs2070951 might be a risk factor for aggressive behavior in the Central South Chinese Han population PMID: 28686058
  50. Glucocorticoid receptor (GR) is recruited to activator protein-1 (AP-1) target genes in a DNA-binding-dependent manner. PMID: 28591827
  51. NR3C1 mean methylation was higher among women who reported childhood abuse PMID: 27620456
  52. observed in almost all cell types of chorionic villous tissue PMID: 27697223
  53. NR3C1 polymorphism is associated with metabolic syndrome. PMID: 27507764
  54. The data of this study show a significant increase in DNA methylation of NR3C1 in peripheral blood mononuclear cells of major depressive disorder patients. PMID: 28246044
  55. Polymorphisms of the glucocorticoid receptor gene influenced both the basal state of the hypothalamus-pituitary-adrenal axis as well as self-perceived stress. The mineralocorticoid receptor gene only associated with self-perceived stress and 5-HTT only with the cortisol awakening response. PMID: 27427534
  56. results reveal that liganded GR spatiotemporally controls ANGPTL4 transcription in a chromosomal context. PMID: 28056052
  57. NR3C1 is a bona fide target of miR-124 in acute lymphoblastic leukemia. PMID: 28578002
  58. Numerous direct transcriptional targets of GR exist in airway smooth muscle. Genes with inducible GR occupancy included IRS2, APPL2, RAMP1, and MFGE8. GR occupancy occurred in the absence of supplemental ligand, including robust GR binding peaks within the IL11 and LIF loci. PMID: 28375666
  59. These results demonstrate that BCLI, N363S and ER22/23EK polymorphisms of NR3C1 do not play a pathogenetic role for Adrenal Incidentalomas. PMID: 27649075
  60. NR3C1 is a haploinsufficient tumor suppressor in a subset of blastic plasmacytoid dendritic cell neoplasms (BPDCN). PMID: 27060168
  61. Glucocorticoid receptor polymorphisms are associated with altered glucocorticoid sensitivity and changes in glucose homeostasis, and other metabolic parameters (Review) PMID: 28397446
  62. TR4 binds GR to play an important role in glucocorticoid-directed corticotroph tumor POMC regulation in addition to modulating glucocorticoid actions on other GR targets. PMID: 27253665
  63. individuals carrying the ADHD risk haplotype 9beta of NR3C1 showed significantly more positive relation between stress exposure and ADHD severity than non-carriers. PMID: 27391809
  64. our observed difference in gene regulation between normal N363S SNP carriers and noncarrier controls may underlie the emergence of metabolic syndrome, type 2 diabetes, and cardiovascular disease PMID: 27600822
  65. The structure and conformational dynamics of glucocorticoid receptor in complex with small heterodimer partner is described. PMID: 28396564
  66. The 9beta and BclI polymorphisms of the GR adversely affect the cardiometabolic profile in patients who are in remission after the treatment of Cushing syndrome. PMID: 26873309
  67. our studies suggest that the joint influence of positive and negative sequence signals partition the genome into regions where glucocorticoid receptor can bind and those where it cannot. PMID: 27016732
  68. Hic-5 regulates GR binding site selection by a novel mechanism, exploiting gene-specific requirements for chromatin remodeling enzymes to selectively influence DNA occupancy and gene regulation by a transcription factor. PMID: 28381557
  69. SERPINE1, ANGPLT4, CCL20, and SAA1 as well as the NF-kappaB (p65) binding sites on GR-transrepressed promoters such as IL-1beta, IL-6, and IL-8 Taken together, our data establish ACTN4 as a transcriptional co-regulator that modulates both dexamethasone-transactivated and -transrepressed genes in podocytes. PMID: 27998979
  70. GR directly represses the MDFIC gene, revealing a negative feedback loop by which glucocorticoids limit MDFIC activity. These findings identify a new binding partner for cytoplasmic GR that modulates the receptor transcriptome and contributes to the tissue-specific actions of glucocorticoids. PMID: 28223352
  71. this study shows that children with moderate-to-severe asthma exacerbations who are homozygous for the G allele at the rs41423247 locus in the NR3C1 gene may have a better response to inhaled corticosteroid treatment PMID: 27003716
  72. The authors examined maternal distress and salivary cortisol in relation to fetal movement and heart rate ("coupling") and DNA methylation of three glucocorticoid pathway genes-HSD11B2, NR3C1, and FKBP5-in term placentas. PMID: 27013342
  73. this paper shows that glutathione peroxidase 3 expression can be regulated independently via epigenetic or glucocorticoid receptor-mediated mechanisms in lung cancer cells PMID: 27484907
  74. glomerular glucocorticoid receptor has a role in childhood nephrotic syndrome PMID: 27690709
  75. NR3C1 variations are associated with major depressive disorder in women but not in men. PMID: 27549215
  76. SNPs in NR3C1 were not significantly associated with asthma in patients from the Henan Province. Patients showed higher frequencies of the AA and GG genotypes of Tth111I and the AA genotype of the N363S SNP compared to healthy volunteers, although these differences were not significant. PMID: 27323143
  77. A glucocorticoid receptor haplotype confers increased sensitivity to glucocorticoids and increases the risk of metabolic syndrome. PMID: 27634941
  78. GR is a putative target gene of Kaiso. PMID: 26424557
  79. Subjects carrying the GG genotype of Bcl-1 polymorphism were at increased risk of early suicide when compared to those carrying the CC genotype . individuals carrying the GG genotype (recessive mode) had an increased risk of early suicide relative to the CC or CG genotype. However, there were no differences in the genotype distributions of the NR3C1 Bcl-1 polymorphism between late suicide cases and controls PMID: 27401254
  80. NR3C1 polymorphisms are associated with metabolic syndrome. PMID: 26596278
  81. The NR3C1 rs852977 polymorphism is a potential marker for genetic susceptibility to non-obstructive azoospermia in Japanese men PMID: 26556219
  82. Data, including data from in silico/molecular docking studies, suggest that high molecular weight phthalates, environmental pollutants, cause endocrine disruption by high-affinity interacts with specific ligand-binding sites on ketosteroid receptors such as AR (androgen receptor), PR (progesterone receptor), and GR (glucocorticoid receptor). PMID: 26304264
  83. Logistic regression revealed that four variants in NR3C1 and SATB1 were significantly associated with lung cancer risk after false discovery rate (FDR) correction [For NR3C1, rs9324921: odds ratio (OR)=1.23, P for FDR=0.029; rs12521436: OR=0.85, P for FDR=0.040; rs4912913: OR=1.17, P for FDR=0.040; For SATB1, rs6808523: OR=1.33, P for FDR=0.040]. PMID: 27179949
  84. properties of the glucocorticoid receptor contribute to the processing of emotional stimuli and influence the intensity of their processing even in the absence of acute stressors. PMID: 26689331
  85. In a Caucasian Greek multiple sclerosis population, polymorphisms in the NR3C1 gene were detected but did not reveal the presence of mutations in any patients. PMID: 27000245
  86. these results demonstrate that patterns of genomic GR occupancy observed with chromatin immunoprecipitation sequencing (ChIP-seq) reflect locally coordinated and functionally synergistic GR binding events, rather than independent and additive events. PMID: 27565349
  87. hyperactive hypothalamo-pituitary-adrenocortical axis in overweight diabetic subjects may be associated with downregulation of 11beta-HSD1, MR, and GR in the brain. PMID: 26212138
  88. Data suggest that cooperative anti-inflammatory gene regulation by glucocorticoid receptor (GR) and the p65 subunit of NF-kappaB contributes to glucocorticoid (GC) efficacy. PMID: 27076634
  89. In conclusion, no correlation was found between rs41423247 polymorphism and GC resistance in our study. We also failed to detect polymorphism of rs56149945 and rs6189/rs6190 among Chinese patients with bullous diseases. PMID: 25894104
  90. Methylation of NR3C1 at exons 1D and 1F is associated with internalizing but not externalizing, behavior problems; and it mediates effects of early adversity in preschoolers. PMID: 26822445
  91. NR3C1 methylation and maternal smoking during pregnancy are independently associated with lethargic behavior in newborns. PMID: 26822442
  92. Significant effects of maternal depressive symptoms on NR3C1 CpG 2 DNA methylation. PMID: 26822444
  93. This report of lower methylation levels in NR3C1 in externalizing disorders may indicate a mechanism through which the differential development of externalizing disorders as opposed to depressive disorders might occur. PMID: 25894927
  94. preschoolers with recurrent wheezing and positive Asthma Predictive Index (API) have higher levels of urinary leukotriene E4 than those with negative API; however, there were no differences in the prevalence of Bcl I polymorphism in the GCR between groups PMID: 25982579
  95. The results reveal molecular defects of pathologic mutant receptors and provide important insights to the actions of wild-type GR. PMID: 26745667
  96. The hGRalphaT556I causes Chrousos syndrome by impairing multiple steps of the glucocorticoid signal transduction pathway. PMID: 26541474
  97. Higher levels of methylation at the NR3C1 promoter may be associated with major depressive disorder in a gender-specific manner. PMID: 26782558
  98. The glucocorticoid receptor A3669G SNP is not associated with polycythemia vera, essential thrombocythemia or primary myelofibrosis PMID: 25926062
  99. Activation of the H1R by its full agonists resulted in a composite potentiating effect. Intriguingly, inactivation of the Gaq-PLC pathway by H1R inverse agonists resulted also in a potentiation of GR activity. PMID: 26635083
  100. Infants with the high-risk neurobehavioral profile showed more methylation than infants with the low-risk neurobehavioral profile at CpG3 for NR3C1 and less methylation of CpG3 for HSD11B2. PMID: 26585459

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Involvement in disease Glucocorticoid resistance, generalized (GCCR)
Subcellular Location Isoform Alpha: Cytoplasm, Nucleus, Mitochondrion, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Note=After ligand activation, translocates from the cytoplasm to the nucleus, SUBCELLULAR LOCATION: Isoform Beta: Nucleus, Cytoplasm, Note=Expressed predominantly in the nucleus with some expression also detected in the cytoplasm, SUBCELLULAR LOCATION: Isoform Alpha-B: Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node
Database Links

HGNC: 7978

OMIM: 138040

KEGG: hsa:2908

STRING: 9606.ENSP00000231509

UniGene: Hs.122926


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