Phospho-STAT5B (Ser731) Antibody

Datasheet
Code CSB-PA220945
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P51692
Image
  • Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using STAT5B (phospho-Ser731) antibody. The lane on the right is treated with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5B (phospho-Ser731) antibody. The picture on the right is treated with the synthesized peptide.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around phosphorylation site of serine 731 (A-P-S(p)-P-A) derived from Human STAT5B.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous levels of STAT5B only when phosphorylated at serine 731.
Tested Applications ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100
Relevance Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias Signal transducer and activator of transcription 5B
Immunogen Species Homo sapiens (Human)
Target Names STAT5B
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Carries out a dual function
Involvement in disease Growth hormone insensitivity with immunodeficiency (GHII)
Subcellular Location Cytoplasm, Nucleus
Protein Families Transcription factor STAT family
Database Links

HGNC: 11367

OMIM: 245590

KEGG: hsa:6777

STRING: 9606.ENSP00000293328

UniGene: Hs.595276

Pathway Chemokine signaling pathway
ErbB signaling pathway
Jak-STAT signaling pathway
Necroptosis
Th1 and Th2 cell differentiation
Th17 cell differentiation
AGE-RAGE signaling pathway in diabetic complications

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