SIRT1 Antibody

Code CSB-PA004095
Size US$167
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  • Western Blot analysis of K562 cells using SIRT1 Polyclonal Antibody
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Product Details

Uniprot No. Q96EB6
Target Names SIRT1
Alternative Names 75SirT1 antibody; hSIR2 antibody; hSIRT1 antibody; HST2 antibody; HST2, S. cerevisiae, homolog of antibody; NAD dependent deacetylase sirtuin 1 antibody; NAD dependent protein deacetylase sirtuin 1 antibody; NAD-dependent deacetylase sirtuin-1 antibody; OTTHUMP00000198111 antibody; OTTHUMP00000198112 antibody; Regulatory protein SIR2 homolog 1 antibody; SIR1_HUMAN antibody; SIR2 antibody; SIR2 like 1 antibody; SIR2 like protein 1 antibody; SIR2, S.cerevisiae, homolog-like 1 antibody; SIR2-like protein 1 antibody; SIR2ALPHA antibody; SIR2L1 antibody; Sirt1 antibody; SirtT1 75 kDa fragment antibody; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody; Sirtuin 1 antibody; Sirtuin type 1 antibody
Raised in Rabbit
Species Reactivity Human,Mouse
Immunogen Synthesized peptide derived from Human SIRT1 around the non-phosphorylation site of S47.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

The Protective Effects of Imperatorin on Acetaminophen Overdose-Induced Acute Liver Injury. Z Gao,Oxid Med Cell Longev,2020

Applications: Western Blot
Review: Pretreatment with IMP alleviates APAP overdose-induced oxidative damage with activation of SIRT1 and promotion of the phosphorylation of AMPK.
PMID: 32454943

The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy. Gao P, et al,Kidney & blood pressure research,2018

Sample type: Renal biopsy tissue
Sample species: Human
Application: IHC
Review: IHC staining with MIOX antibody (a and b), Sirt1 antibody (c and d) and DHE staining (e and f). A notable increase in MIOX expression and ROS production was seen in the kidneys of DN patients compared to the control (b vs a and f vs e).
PMID: 330388862

Exogenous angiotensin (1-7) directly inhibits epithelial-mesenchymal transformation induced by transforming growth factor-β1 in alveolar epithelial cells. Min Shao, et al,Biomedicine & Pharmacotherapy,2019

Applications: WB
Antibody dilution factor: 1: 1000
Review: the protein expressions of ACE2 and SIRT1 were detected by western blotting.
PMID: 31387171

Target Data

Function NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell
Gene References into Functions
  1. These results suggest that resveratrol induces chondrosarcoma cell apoptosis via a SIRT1-activated NF-kappaB (p65 subunit of NF-kappaB complex)deacetylation and exhibits anti-chondrosarcoma activity in vivo. PMID: 28600541
  2. I157172 induced upregulation of SIRT1, and downregulation of acetyl-STAT3. PMID: 30365149
  3. SIRT1-mediated H3K9 deacetylation helps to maintain gene repression but is not required for the direct ZEB2 repressive function.SIRT1 activity maintains the stability of ZEB2-induced RAB25 repression. PMID: 30445998
  4. Data show that long non-coding RNA MALAT1 (MALAT1) repressed sirtuin 1 (SIRT1) expression through targeting forkhead box protein O1 (Foxo1). PMID: 29928873
  5. SIRT1 had a pivotally protective role in the regulation of ADSCs aging and apoptosis induced by H2O2 PMID: 29803744
  6. plasma levels correlated inversely with all studied adiposity and atherogenicity indices in metabolic syndrome patients with and without prediabetes PMID: 29779969
  7. Increased SIRT1 activity protects against diabetes-induced podocyte injury and effectively mitigates the progression of diabetic kidney disease. PMID: 29477240
  8. the Sirt1 carboxyl-domain is an ATP-repressible domain that is transferrable to other proteins PMID: 28504272
  9. Adipose tissue sirtuin 1 was related to insulin sensitivity. The relationship was still present after controlling for BMI, however, it disappeared after controlling for adipose tissue SLC2A4. Muscle sirtuin 1 was not related to insulin sensitivity. PMID: 29417372
  10. data suggest that SIRT1 is an oncogenic factor in breast cancer cells and can be involved in the progression of breast cancer by inhibiting p53 and activating POLD1 PMID: 29807012
  11. SIRT1 expression is significantly upregulated in paclitaxel-resistant cervical cancer tissues and cell lines compared to normal tissues or PTX-sensitive CC tissues and cell lines. Knockdown of SIRT1 inhibited the cell proliferation, promoted cell cycle arrest and apoptosis of PTX-sensitive CC cells, and decreased the expression of MDR proteins. PMID: 29808798
  12. In our retrospective study, high SIRT1 expression significantly correlated with vascular invasion and a worse prognosis in colorectal cancer PMID: 30082156
  13. SIRT1 polymorphisms and their expression were associated with the presence of alcoholic fatty liver disease (AFLD), and there was a close relationship among four SNPs and body mass index in AFLD patients, but no SNP was related to its expression. PMID: 29189974
  14. The variable role of SIRT1 in the maintenance and differentiation of mesenchymal stem cells. PMID: 29715067
  15. Results indicate that SIRT1 may promote the metastasis of chondrosarcoma by inducing epithelial-mesenchymal transition and can be a potential molecular target for chondrosarcoma therapy. PMID: 28112277
  16. Regulation of transmembrane-4-L-six-family-1 (TM4SF1) on bladder cancer cell could be induced by peroxisome proliferator-activated receptor gamma (PPARgamma)-sirtuin 1 (SIRT1) feedback loop. PMID: 29175458
  17. Results present evidence that SIRT1 plays an essential role in regulating the transcription of CLDN5 likely by modifying and modulating the activity of KLF4 in ovarian cancer cells. PMID: 28888043
  18. results suggest that seminal SIRT1 expression has a role in male infertility PMID: 29359516
  19. SIRT1 may promote the transformation of tumor cells by inducing the epithelial-mesenchymal transition. PMID: 29656187
  20. relevance of the discovered Sirt1-Smad2 interaction for the regulation of TGFbeta-dependent gene transcription PMID: 29187201
  21. Bioinformatics and the dual luciferase reporter assay analysis results demonstrated that miR-29a specifically targeted the 3'-UTR of SIRT1 mRNA and regulated its protein expression. Increased SIRT1 expression rescued the inhibited effects induced by miR-29a overexpression in HCC cells. PMID: 29630527
  22. High Sirt1 Expression is associated with Gastric cancer. PMID: 29693338
  23. Sirt1 protects against oxidative stress-induced apoptosis in fibroblasts from psoriatic patients. PMID: 29799444
  24. Investigated the effect of statins on the expression of sirtuin 1 (SIRT1) and endothelial nitric oxide synthase 3 (eNOS) proteins in young premature myocardial infarction (PMI) patients. Found patients with PMI who were taking statins had a markedly higher level of SIRT1 compared with the controls. The level of eNOS protein was considerably lower in PMI patients compared with the control group. PMID: 29664427
  25. Clinical data are the first to identify SIRT1 as an important regulator of hepatocellular function in human liver transplants under ischemia/reperfusion stress. PMID: 28719070
  26. suggests SIRT1 may serve as a predictor of poor prognosis in esophageal squamous cell carcinoma, and its mediated tumor-promoting role might be associated with the overexpression of EGFR protein in esophageal squamous cell carcinoma PMID: 29625788
  27. findings demonstrate a new mechanism for the activation of SIRT1 under stress conditions and suggest a novel potential therapeutic target for preventing age-related diseases and extending healthspan PMID: 29133780
  28. This study represents the important role of Sirt1 and senescence in the regulation of beige adipocyte differentiation during aging. PMID: 29678576
  29. Baicalin activated the SIRT1/AMPK and mechanistic target of rapamycin (mTOR), and SIRT1/AMPK and matrix metalloproteinase (MMP) signaling in A549 and H1299 cells in a dose-dependent manner. siRNA silencing of SIRT1 and AMPK reduced the effects of baicalin on cell proliferation and migration. PMID: 29632297
  30. We provide a comprehensive overview of recent developments on the molecular signaling pathways controlled by SIRT1 and SIRT6, two post-translational modifiers proven to be valuable tools to dampen inflammation and oxidative stress at the cardiovascular levell PMID: 28661724
  31. SIRT1 was identified as a direct target of miR-212 and its expression was inversely correlated with miR-212 expression in thyroid cancer tissues. Overexpression of SIRT1 could effectively rescue miR-212 mimic-induced suppression of cell proliferation, migration and invasion in TPC-1 thyroid cancer cell line. PMID: 29207181
  32. The present study is the first to report a significant association between SIRT1 polymorphisms and antisocial personality in adolescents. PMID: 28439078
  33. Studied effects of dexamethasone on gene expression regulation of sirtuin 1 (SIRT1), interleukin 6 (IL6), and endothelin 1 (EDN1) in gingival derived aging stem cells. Dexamethasone downregulated expression of SIRT1 and IL6 but upregulated EDN1 in stem cells. PMID: 29302812
  34. miR-200a and its target gene, SIRT1, may exert a possible role in induction of apoptosis in dopaminergic neurons. PMID: 29936262
  35. The rhuschalcone I analogue (9) showed the best activity against sirt1, with an IC50 value of 40.8 microM. Based on the docking experiments, suggestions for improving the biological activities of the newly identified hit compounds have been provided. PMID: 29443909
  36. Results indicated that SIRT1 contributes to the neuroprotection of salidroside against MPP(+) -induced apoptosis and oxidative stress, in part through suppressing of mitogen-activated protein kinase (MAPK) pathways. PMID: 28851138
  37. decreased SIRT1 expression and its SUMOylation by SUMO1 and SUMO2/3 may be associated with the development of bronchopulmonary dysplasia. PMID: 29115559
  38. The nicotinamide adenine dinucleotide (NAD)-dependent deacetylase SIRT1 acts as an energy sensor and negatively regulates poly(A)RNA transport via deacetylating a poly(A)-binding protein, PABP1. PMID: 28756945
  39. L-Carnitine alleviated epithelial mesenchymal transformation-associated renal fibrosis caused by perfluorooctanesulfonate through a Sirt1- and PPARgamma-dependent mechanism. PMID: 28973641
  40. High SIRT1 expression is associated with hepatocellular carcinoma. PMID: 28677784
  41. No significant association has been discovered between SIRT1 polymorphisms and diabetic foot severity or characteristics PMID: 29995800
  42. A significant correlation between the physical activity level and peripheral blood mononuclear cell SIRT1 and FOXO1 mRNA expression was found in COPD patients. PMID: 29138552
  43. Defective sirtuin-1 was found to increase IL-4 expression through acetylation of GATA-3 in patients with severe asthma compared with healthy controls. PMID: 26627546
  44. SIRT1 gene polymorphisms can have direct and indirect effects on the pathogenesis of coronary artery diseases. PMID: 29885463
  45. miR-146 exerted protective functions might be via up-regulation of Sirt1 thereby blocking NF-kappaB and Notch pathways. PMID: 29229881
  46. results suggested that SIRT1 deficiency in Bladder cancer cells could suppress cell viability by activating antioxidant response and inducing cell cycle arrest possibly via FOXO3a-related pathways. PMID: 29147649
  47. These observations provide further evidence for a critical tumor-suppressive role of the miR-200a in renal cell carcinoma (RCC) in addition to identifying a novel regulatory mechanism, which may contribute to SIRT1 upregulation in RCC. PMID: 28717923
  48. Results showed that RSV or overexpression of SIRT1 elicited inhibitory effects on NMDA-induced excitotoxicity including a decrease in cell viability, an increase in lactate dehydrogenase (LDH) release, and a decrease in the number of living cells as measured by CCK-8 assay, LDH test, and Calcein-AM and PI double staining. PMID: 29081884
  49. results indicate that FOXO1 inhibits gastric cancer (GC) growth and angiogenesis under hypoxic conditions via inactivation of the HIF-1alpha-VEGF pathway, possibly in association with SIRT1; thus, development of treatment modalities aiming at this pathway might be useful for treating GC PMID: 25761483
  50. Data suggest the activity restoration role of resveratrol toward some "loose-binding" substrates of sirtuin 1 (SIRT1), and has implications for the rational design of new substrate-specific SIRT1 modulators. PMID: 27901083
  51. Here we have shown that Resveratrol promoted human embryonic stem cells (hESCs)proliferation through cell cycle modulation and up-regulation of anti-apoptotic markers, without affecting pluripotency. Furthermore, inhibition of SIRT1 by EX-527 resulted in suppression of Resveratrol-induced enhancement of hESCs self-renewal. PMID: 28865806
  52. examined the effects of the SIRT1 activator CAY10591 in gingival epithelial carcinoma Ca9-22 cells. CAY10591 treatment dose- and time-dependently increased SIRT1 level and activity PMID: 28904318
  53. JNK inhibitor prevents SIRT1 phosphorylation, leading to elevated SIRT1 protein levels even in the presence of H2O2. Taken together, our results indicate that CHFR plays a crucial role in the cellular stress response pathway by controlling the stability and function of SIRT1. PMID: 27883020
  54. Wnt/beta Catenin signaling is suppressed by Sirt1 in liver cancer cells. PMID: 28583374
  55. SIRT1, a member of the sirtuin family, is expressed in human ovarian tissue and granulosa cells (GCs). SIRT1 protects against GC apoptosis via activation of ERK1/2, decreasing the level of the anti-apoptotic protein Bcl-2, and elevating the level of apoptosis-related protein caspase-3. SIRT1 also inhibits GC apoptosis by inhibiting nuclear factor (NF)-kappaB/p65 protein accumulation in the nucleus. PMID: 28755171
  56. mitochondria elongation under hypoxic condition is regulated through SIRT1-mediated MFN1 deacetylation and accumulation. PMID: 28669827
  57. Regulation of stem cell aging by SIRT1 occurs via linking metabolic signaling to epigenetic modifications. (Review) PMID: 28392411
  58. Nrf2 and SIRT-1 are activated by steroid like phytochemical Antcin M, an anti-aging reagent that eliminates hyperglycemia-accelerated premature senescence in dermal fibroblasts PMID: 27542238
  59. Transcriptional coactivator p300 gene polymorphism correlates with the development and advancement of diabetic kidney disease. Additionally, the SIRT1 gene collaborates with the p300 gene and participates in promoting albuminuria in type 2 diabetes mellitus patients. PMID: 28444663
  60. SIRT1 inhibits the transcriptional activity of Nkx2.5. PMID: 27819261
  61. The interplay between Sirt1 and Per2 modulates aging gene expression and circadian-clock maintenance. PMID: 27346580
  62. SIRT1 expression levels were repressed in the monocytes of patients with coronary artery disease. PMID: 28601780
  63. our study showed that SIRT1 is indispensable for hESC survival and that the modulation of the levels of multiple DNA repair proteins is at least partially responsible for the SIRT1-mediated prevention of cell death PMID: 28689995
  64. The findings of the present study suggest that mTOR signaling promotes foam cell formation and inhibits foam cell egress via suppression of SIRT1 signaling. PMID: 28765952
  65. present study revealed that hyperglycemia promotes oxidative stress, mitochondrial dysfunction and apoptosis in HUVECC cells, and manipulation of SIRT1 activity regulated hyperglycemiainduced mitochondrial dysfunction and apoptosis in HUVECC cells. The data revealed the protective effect of SIRT1 against hyperglycemiainduced apoptosis via the alleviation of mitochondrial dysfunction and oxidative stress. PMID: 28765962
  66. The present findings implied that the low-concentration preconditioning of BBR could have a mitohormetic effect against cellular senescence triggered by oxidative stress in some age-related diseases through the regulation of SIRT1. PMID: 28751929
  67. Study showed that Sirt1 expression was induced in the inflammatory phase of systemic sclerosis. PMID: 28532580
  68. FOXQ1 remarkably inhibited the replicative senescence through depressing the expression of the inflammatory cytokines interleukin-6 (IL-6) and IL-8 via modulation of SIRT1-NF-kappaB pathway PMID: 28726780
  69. Results of the present study suggest a positive role for SIRT1 in the development of OvCa and its potential as a novel therapeutic target PMID: 26862948
  70. SIRT1 which is vital in the evolution of renal Tubulointerstitial fibrosis in diabetic nephropathy can be directly suppressed by miR-34a-5p. PMID: 29371016
  71. Sirt1 is a protective mediator in intervertebral disc degeneration and the expression of Sirt1 decreases in degenerative disc. PMID: 27792110
  72. strongly suggest that CRIF1 deficiency promotes endothelial cell inflammation by increasing VCAM-1 expression, elevating inflammatory cytokines levels, and activating the transcription factor NF-kappaB, all of which were inhibited by SIRT1 overexpression PMID: 29474366
  73. upregulation of Sirt1 expression may inhibit OA chondrocyte apoptosis and extracellular matrix degradation by increasing Bcl2 expression and decreasing Bax, MMP1 and MMP13 expression, via downregulation of p38, JNK and ERK phosphorylation. PMID: 28586000
  74. LAT1-NAD+-SIRT1 signaling is activated in tumor tissues of patients with non-small cell lung cancer; NAD+ synthesis regulates the SIRT1-FOXO1 apoptotic pathway in response to NQO1 PMID: 27566573
  75. Our data indicate that there exist frameshift mutations of CUX1 and SIRT1 genes as well as intratumoral heterogeneity of CUX1 frameshift mutation in high microsatellite instability cancers, which together might play a role in tumorigenesis of gastric cancer and colorectal cancer with high microsatellite instability PMID: 29363918
  76. Studied melatonin's role in cell senescence, autophagy, sirtuin 1 expression and acetylation of RelA in hydrogen peroxide treated SH-SY5Y cells. PMID: 28295567
  77. We showed that SIRT1 inhibition blunted autophagy induction in response to nicotinamide supplementation and similarly abrogated the anti-lipotoxic effect conferred by nicotinamide supplementation. PMID: 28473059
  78. Data suggest that aberrant expression of hypermethylated in cancer-1 (HIC1)/sirtuin-1 (SIRT1) and hypermethylation of the HIC1 promoter may be critical for the development and progression of papillary thyroid cancer. PMID: 27793057
  79. Ultraviolet B rays suppressed SIRT1 expression by activating AhR, and subsequently inhibited DNMT1 activity in CD4+ T cells from systemic lupus erythematosus patients. PMID: 28336124
  80. It may inhibit the proliferation, invasion and migration of lung cancer through downregulation of Sirt1 expression and activation of cell autophagy. The downregulation of miR-138 is closely related to the development of lung cancer PMID: 28498463
  81. Results show that CRIF1 deficiency leads to downregulated SIRT1 levels accompanied with increased eNOS acetylation and decreased nitric oxide production that promote impaired vascular function. eNOS is identified as an SIRT1 substrate under oxidative stress. PMID: 28117598
  82. SIRT1-mediated downregulation of p27Kip1 is essential for overcoming contact inhibition of Kaposi's sarcoma-associated herpesvirus transformed cells. PMID: 27708228
  83. These findings will shed light on the role and mechanism of miR-133b in regulating osteosarcoma cell growth via the miR-133b/Sirt1 axis. PMID: 28059051
  84. SIRT1 expression per se was not prognostic but low expression of Cell Cycle and Apoptosis Regulator 2 (CCAR2), a reported SIRT1 inhibitor, corresponded to poor patient survival. PMID: 27494892
  85. High tumor levels of SIRT1 may be a biomarker for a high pulmonary metastatic rate in osteosarcoma patients. PMID: 27793039
  86. High SIRT1 expression is associated ovarian cancer. PMID: 26992208
  87. results show that a loss of SIRT1 may participate in the pathogenesis of systemic sclerosis-related pulmonary fibrosis, and that SIRT1 activation is an effective treatment for both the early (inflammatory) and late (fibrotic) stages of pulmonary fibrosis. PMID: 28800254
  88. celecoxib and sulindac can inhibit TGF-beta1-induced epithelial-mesenchymal transition and suppress lung cancer cell migration and invasion via downregulation of SIRT1 PMID: 27528025
  89. SIRT1 attenuate cardiac dysfunction and improve DCP. PMID: 28380414
  90. Up-regulating AMPK and SIRT1 signal pathways. PMID: 28364603
  91. Taken together, our results indicated that miR-449 overexpression inhibited osteogenic differentiation of HG-FFA-treated hBMSCs through the Sirt1/Fra-1 signal pathway. PMID: 29305863
  92. The influence of diets on metabolic processes associated with sirtuin1. PMID: 29381014
  93. these results demonstrate that the reversible acetylation of FOXM1 by p300/CBP and SIRT1 modulates its transactivation function PMID: 27542221
  94. miR-212 facilitates macrophage foam cell formation and suppresses ABCA1-dependent cholesterol efflux through downregulation of SIRT1. PMID: 29174964
  95. By downregulating acetylated LKB1 protein via HERC2, SIRT1 fine-tunes the crosstalk between endothelial and vascular smooth muscle cells to prevent adverse arterial remodeling and maintain vascular homeostasis PMID: 27259994
  96. Findings indicated that the miR-29b/SIRT1 axis has a protective effect against H2O2-induced damage of cell viability and oxidative stress and may provide novel options for miR-29b-based therapeutic approaches for EOC treatment. PMID: 29050034
  97. High SIRT1 expression is associated with liver cancer. PMID: 26967560
  98. Our findings clearly evidenced a novel and negative role of miR-199b in the regulation of SIRT1 in colorectal cancer cells PMID: 27145368
  99. We found a negative correlation between mRNA levels of SIRT1 in VAT of obese individuals and SIRT7 in VAT of the normal-weight subjects and expression of the relevant miRNAs. PMID: 27480132
  100. SIRT1 activation induces STAT3 deacetylation, reducing its ability to translocate into the nucleus, bind to Rorc promoter, and induce its transcription. PMID: 28445726

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Subcellular Location Nucleus, PML body, Cytoplasm, Nucleus
Protein Families Sirtuin family, Class I subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 14929

OMIM: 604479

KEGG: hsa:23411

STRING: 9606.ENSP00000212015

UniGene: Hs.369779

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