Recombinant Arabidopsis thaliana 3-phosphoinositide-dependent protein kinase 1(PDPK1)

Code CSB-YP894967DOA
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Source Yeast
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Code CSB-EP894967DOA
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Source E.coli
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Code CSB-EP894967DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894967DOA
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Source Baculovirus
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Code CSB-MP894967DOA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names PDPK1
Uniprot No. Q9XF67
Alternative Names PDPK1; PDK1; At5g04510; T32M21.110; 3-phosphoinositide-dependent protein kinase 1; AtPDK1; EC 2.7.11.1
Species Arabidopsis thaliana (Mouse-ear cress)
Expression Region 1-491
Target Protein Sequence MLAMEKEFDS KLVLQGNSSN GANVSRSKSF SFKAPQENFT SHDFEFGKIY GVGSYSKVVR AKKKETGTVY ALKIMDKKFI TKENKTAYVK LERIVLDQLE HPGIIKLYFT FQDTSSLYMA LESCEGGELF DQITRKGRLS EDEARFYTAE VVDALEYIHS MGLIHRDIKP ENLLLTSDGH IKIADFGSVK PMQDSQITVL PNAASDDKAC TFVGTAAYVP PEVLNSSPAT FGNDLWALGC TLYQMLSGTS PFKDASEWLI FQRIIARDIK FPNHFSEAAR DLIDRLLDTE PSRRPGAGSE GYVALKRHPF FNGVDWKNLR SQTPPKLAPD PASQTASPER DDTHGSPWNL THIGDSLATQ NEGHSAPPTS SESSGSITRL ASIDSFDSRW QQFLEPGESV LMISAVKKLQ KITSKKVQLI LTNKPKLIYV DPSKLVVKGN IIWSDNSNDL NVVVTSPSHF KICTPKKVLS FEDAKQRASV WKKAIETLQN R
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
May couple lipid signals to the activation-loop phosphorylation of several protein kinases of the so-called AGC kinase family. Interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P and PtdIns(3,4)P2 and to a lesser extent with PtdIns(4,5)P2 and PtdIns4P. May play a general role in signaling processes controlling the pathogen/stress response, polar auxin transport and development. Transphosphorylates the AGC protein kinases OXI1/AGC2-1, PK1/S6K1, PK19/S6K2 and PID resulting in their activation.
Gene References into Functions
  1. PDK1 undergoes autophosphorylation at several sites; mutation of Ser-276 to Ala resulted in enzyme with no detectable autophosphorylation; other sites important for autophosphorylation &/or activity were Asp-167, Thr-176, & Thr-211 PMID: 16125835
Subcellular Location Cytoplasm. Membrane; Peripheral membrane protein.
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily
Tissue Specificity Ubiquitous.
Database Links

KEGG: ath:AT5G04510

STRING: 3702.AT5G04510.1

UniGene: At.4924

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