Recombinant Bovine Neutrophil cytosol factor 1 (NCF1)

Code CSB-YP015525BO
MSDS
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Source Yeast
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Code CSB-EP015525BO
MSDS
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Source E.coli
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Code CSB-EP015525BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015525BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP015525BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NCF1
Uniprot No.
Alternative Names
NCF1; Neutrophil cytosol factor 1; NCF-1; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; p47-phox
Species
Bos taurus (Bovine)
Expression Region
1-392
Target Protein Sequence
MGDHFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKILKEMFPI EAGDINPENR IIPHLPAPRW YDGQRVAESR QGTLTEYCST LMSLPVKISR CPHLLNFFKV RPDDLKLPTD SQVKKPETYL MPKDGKNNAA DITGPIILQT YRAIADYEKG SSSQMALATG DVVDVVEKNE SGWWFCQMKT KRGWVPASYL EPLDSPDEAE DPEPNYAGEP YITIKAYTAV LEDEISLEEG EAIEVIHKLL DGWWVIRKED VTGYFPSMYL QKAGQDVAQA KSQIKSRGAP PRRSSIRNAH SIHQRSRKRL SQDTYRRNSV RFMQQRRHQR PEPQRSRSAL REQQQPKTER PKPQPAVPPR PSADLILHRC SESTKRKLAS AV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Gene References into Functions
  1. Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress. PMID: 26826128
  2. two novel splice variants designed as NCF1-TV1 (retained intron 6) and NCF1-TV2 (retained part of intron 8)encoding two putative truncated proteins (239AA and 283AA). Two splice variants were up-regulated in the mastitis-infected cows' mammary tissues, blood and neutrophils compared with these of healthy cows. PMID: 25946326
  3. A p47(phox) and Src kinase activation of peroxide production by Nox2 appears to be an important contributor to vascular contractile mechanisms mediated through activation of protein kinase C. PMID: 19168729
Subcellular Location
Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side.
Database Links

KEGG: bta:281345

STRING: 9913.ENSBTAP00000004279

UniGene: Bt.142

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